Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,066 G→C 6.0% intergenic (+114/‑128) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0660A→C 6.0% 23.8 ‑0.6 ‑0.4227intergenic (+114/‑128)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (115/91):  total (126/101)
Fisher's exact test strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.77e-01

Only 100 of 236 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.AG.CAGAAGGGGGGCAAGCG.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493977‑2494152
                                                                                               |                                                                                         
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagc                                                                               <  1:3524300/101‑3 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagc                                                                               >  2:5420299/1‑99 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcg                                                                              >  2:3608282/1‑98 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcg                                                                              >  2:4364566/1‑98 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcgc                                                                             >  2:7107447/1‑97 (MQ=18)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                          >  2:30725/1‑101 (MQ=18)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                          >  1:2701939/1‑101 (MQ=18)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCaccgcccc                                                                          >  2:5968552/1‑94 (MQ=18)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.c                                                                     >  2:1641865/1‑101 (MQ=17)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cc                                                                    >  1:222273/1‑100 (MQ=17)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGG.AGGCG.cc.cc.cc                                                                    >  2:2902251/1‑100 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cca                                                                   <  2:5732257/101‑3 (MQ=17)
            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cctgc                                                                 <  2:4881664/101‑5 (MQ=17)
             ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cctgcc                                                                <  1:994628/101‑6 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGcccc                                                               >  1:6612003/1‑101 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGcccc                                                               >  2:1766453/1‑101 (MQ=17)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGCCCcg                                                              >  1:2857575/1‑101 (MQ=2)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcg                                                              <  2:5653150/101‑1 (MQ=17)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGCCCcgcc                                                            <  1:6014660/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggc                                                            <  2:2034199/101‑1 (MQ=17)
                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggcg                                                           >  1:6142998/1‑101 (MQ=17)
                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggcggc                                                         >  1:1611925/1‑100 (MQ=17)
                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGt                                                         <  1:6135596/101‑1 (MQ=17)
                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGt                                                         <  2:6449675/101‑1 (MQ=17)
                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGCCCcgccggat                                                        <  1:5945681/101‑3 (MQ=2)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       <  1:6524599/101‑1 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       <  2:2441416/101‑1 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       >  1:3578887/1‑101 (MQ=18)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGG.C..GAAGCCAGAGGGGGGACGGGC..CC.CC.CGGCCCCGGCGGTcc                                                       >  2:4252375/1‑101 (MQ=17)
                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTgg                                                   >  1:4493287/1‑101 (MQ=18)
                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTgg                                                   >  1:5794103/1‑101 (MQ=18)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTggc                                                  >  2:3891263/1‑101 (MQ=18)
                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGCCCGTggcg                                                 >  1:3383889/1‑101 (MQ=17)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTggcgg                                                >  1:3811002/1‑101 (MQ=17)
                               gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTggcggc                                               <  1:6883905/101‑1 (MQ=17)
                               gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGGCCGGggcggc                                               >  1:3877195/1‑101 (MQ=17)
                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGgcgc                                             >  2:3155880/1‑101 (MQ=18)
                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCAGTGGCGgcgc                                             >  2:605370/1‑101 (MQ=17)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                            <  2:6581054/101‑1 (MQ=17)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGGGGCGGCGcc                                            >  1:2865893/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGCCCGTCGCGGCGCCt                                           >  2:1856317/1‑101 (MQ=14)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:5769379/101‑1 (MQ=17)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:4070976/101‑1 (MQ=17)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGACC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  2:4633550/1‑101 (MQ=18)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  1:4201836/1‑101 (MQ=17)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATGCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:229743/101‑1 (MQ=17)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:4103792/101‑1 (MQ=17)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:1348192/101‑1 (MQ=17)
                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        >  2:4431943/1‑101 (MQ=18)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:3499855/101‑1 (MQ=17)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGCGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:3456692/101‑1 (MQ=17)
                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCGTGAcg                                      >  1:3440069/1‑101 (MQ=17)
                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:2494285/1‑101 (MQ=18)
                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     <  2:3541273/101‑1 (MQ=17)
                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    >  2:6118784/1‑101 (MQ=18)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  1:1574503/101‑1 (MQ=17)
                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:4196347/1‑101 (MQ=17)
                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 >  1:4061165/1‑101 (MQ=18)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  2:3644775/1‑101 (MQ=18)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAccggcggg                                >  2:412531/1‑101 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  1:6252574/1‑101 (MQ=17)
                                               tttGATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CCGCCCCGGCGGTCCGTGTCGGCGGCTTGACGGGCgggg                               >  1:5613427/1‑101 (MQ=9)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCAGCTC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              <  1:1009584/101‑1 (MQ=18)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              <  1:1828257/101‑1 (MQ=18)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  1:740644/1‑101 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCC..A.AG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             >  2:2498333/1‑101 (MQ=18)
                                                 tCATCTGGCCCGAGATGTCGCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:6428046/101‑1 (MQ=16)
                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  2:3206132/1‑101 (MQ=18)
                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.CCGCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  1:6434785/1‑101 (MQ=18)
                                                    tcgtGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:1837078/98‑1 (MQ=18)
                                                    cttggCCCGAAATTACCCGGGGGGGGGA.AGG.CG.G.AG.CCCAGGGGGGGGCAGCC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  1:3611082/97‑1 (MQ=255)
                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         <  2:1170636/101‑1 (MQ=18)
                                                      ttGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        <  2:316532/101‑1 (MQ=18)
                                                      ttGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        >  1:4032261/1‑101 (MQ=18)
                                                        gCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCAGCAC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  1:5227265/1‑101 (MQ=21)
                                                        gCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCa                      >  1:3185389/1‑101 (MQ=18)
                                                         cccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     >  2:2824829/1‑101 (MQ=18)
                                                         cccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  2:5242518/101‑1 (MQ=18)
                                                         cccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  1:4732130/101‑1 (MQ=18)
                                                          ccGAAATATCCCGGGGGGAGAA.TGG.C..GAAG.CA.AGGGGGGGGCAGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:1136679/101‑1 (MQ=21)
                                                            gAAATCGCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:2631363/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:1978730/101‑1 (MQ=18)
                                                             aaaTATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  2:2673316/1‑101 (MQ=18)
                                                               attacccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               <  2:6304074/97‑1 (MQ=21)
                                                               atatCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               <  1:2414890/101‑1 (MQ=21)
                                                                tatCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:4738294/1‑101 (MQ=21)
                                                                   cccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGGCCCGGCGGGCCGTGGCGGCGCGTTGGCGGGCGGGGGGGCGGGCGGTAAccagacg           >  2:2270117/1‑96 (MQ=9)
                                                                   cccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt           >  1:1714053/1‑101 (MQ=21)
                                                                    ccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:1322901/101‑1 (MQ=21)
                                                                    ccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:3084703/101‑1 (MQ=21)
                                                                    ccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:6147874/101‑1 (MQ=21)
                                                                     cGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGCAATTg         >  2:5065239/1‑101 (MQ=21)
                                                                      ggggggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:3245133/1‑101 (MQ=25)
                                                                      ggggggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:4044295/1‑101 (MQ=25)
                                                                       gggggTGAA.TGG.C..GAAGCCAG.AGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  2:6177567/1‑101 (MQ=25)
                                                                         gggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:2053421/101‑1 (MQ=25)
                                                                          ggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  2:3840326/101‑1 (MQ=25)
                                                                          ggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:3354257/101‑1 (MQ=25)
                                                                          ggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:4227174/101‑1 (MQ=25)
                                                                            tGAA.TGG.C..GAAG.CCAGAGGGCGGC.AGCG.CC.CCTCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  <  1:4654149/101‑1 (MQ=37)
                                                                                               |                                                                                         
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.AG.CAGAAGGGGGGCAAGCG.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493977‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG