Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,982,999 A→G 100% V81A (GTG→GCG)  hslR ← heat shock protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,982,9990A→G100.0% 1297.7 350V81A (GTG→GCG) hslRheat shock protein
Reads supporting (aligned to +/- strand):  new base (207/143):  ref base (0/0):  total (207/143)

Only 100 of 357 total aligned reads displayed.

GGCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCACGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGCC  >  NC_014034/2982901‑2983096
                                                                                                  |                                                                                                 
 gCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCgg                                                                                                >  2:1446566/1‑101 (MQ=255)
    cgggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCtt                                                                                             >  1:6105287/1‑101 (MQ=255)
      ggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTcc                                                                                           >  1:4534785/1‑101 (MQ=255)
      ggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTcc                                                                                           <  1:1195022/101‑1 (MQ=255)
       gcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAATCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCCATCAGCGCGTAGAGCTTCGCGGCTTCCg                                                                                          <  1:5068474/101‑1 (MQ=38)
        cgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTcccg                                                                                         >  2:4325805/1‑99 (MQ=255)
         ggggAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGgc                                                                                        >  1:673553/1‑101 (MQ=255)
           ggAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGgcgc                                                                                      >  2:6908501/1‑101 (MQ=255)
           ggAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGgcgc                                                                                      >  2:1165770/1‑101 (MQ=255)
            gAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGcgcg                                                                                     >  2:6626753/1‑101 (MQ=255)
              aaGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCggg                                                                                   >  1:3128332/1‑101 (MQ=255)
              aaGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCggg                                                                                   <  2:5157084/101‑1 (MQ=255)
               gatctaTGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATTAGCGCGTAGAGCGTCGCGGCTTCCGGCGCgggg                                                                                  <  1:3709354/96‑1 (MQ=255)
                 ccGATGCCTTGCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGcc                                                                                <  2:3328935/100‑1 (MQ=255)
                  cGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCcg                                                                               >  2:4687748/1‑101 (MQ=255)
                    aTGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCcgcg                                                                             >  2:1856567/1‑101 (MQ=255)
                     tGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCgcgg                                                                            >  1:2613729/1‑101 (MQ=255)
                      gCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCgcggc                                                                           <  1:1092945/101‑1 (MQ=255)
                       ccTTCCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCgcggcg                                                                          <  1:6016853/101‑1 (MQ=255)
                        cTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGgcgc                                                                         >  2:3660497/1‑101 (MQ=255)
                         tttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGcgcg                                                                        >  2:2238256/1‑101 (MQ=255)
                          ttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGt                                                                       <  2:5833298/101‑1 (MQ=255)
                          ttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGGCGCGGcgcgg                                                                       >  2:1353370/1‑100 (MQ=255)
                           tCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTg                                                                      >  2:4919416/1‑101 (MQ=255)
                           tCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTg                                                                      >  1:1850476/1‑101 (MQ=255)
                            cccGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGc                                                                     >  2:4217257/1‑101 (MQ=255)
                             ccGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGcc                                                                    <  1:6643281/101‑1 (MQ=255)
                               gaaccGCCGGAAACCGGTTATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGc                                                                  <  2:6960368/98‑1 (MQ=255)
                               gATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGc                                                                  >  1:1621612/1‑101 (MQ=255)
                               gATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGc                                                                  >  1:4047860/1‑101 (MQ=255)
                                 tCCGCCCGAAACCGGGGATGGCCACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAg                                                                <  1:6543175/101‑1 (MQ=255)
                                     ccctgaaCCGGTGATGGCAACGGATCGCCCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCa                                                            <  2:4409781/96‑1 (MQ=255)
                                     cccGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTTCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCa                                                            <  1:5430233/101‑1 (MQ=255)
                                      ccGAAACCGGGGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAg                                                           <  2:2642955/101‑1 (MQ=255)
                                        gAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGa                                                         >  1:3750469/1‑101 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGAt                                                        <  1:4348604/101‑1 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCCCCCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGAt                                                        <  1:6430469/101‑1 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGag                                                        >  2:6325689/1‑100 (MQ=255)
                                           aCCGGTGATGGCAACGGATCCCCCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgc                                                      <  1:4765184/101‑1 (MQ=255)
                                             cGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgcgc                                                    >  1:1391088/1‑101 (MQ=255)
                                              ggTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGACGCAGGCCAGGGTGCgca                                                   >  2:6437038/1‑101 (MQ=255)
                                                 gATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgcagca                                                >  1:2497860/1‑101 (MQ=255)
                                                  aTGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCAc                                               >  1:41326/1‑101 (MQ=255)
                                                  aTGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCAc                                               <  1:1353421/101‑1 (MQ=255)
                                                   tGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCAcg                                              >  1:1014625/1‑101 (MQ=255)
                                                        aCGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCAc                                         <  2:79378/101‑1 (MQ=255)
                                                         cGGATCCCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCAcc                                        <  1:6328153/101‑1 (MQ=255)
                                                          ggATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGGCAGGATGCGCGGCGCGCGCACCt                                       >  1:794803/1‑101 (MQ=255)
                                                          ggATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCAccc                                       >  1:2688151/1‑100 (MQ=255)
                                                          ggATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCt                                       >  2:5986157/1‑101 (MQ=255)
                                                          ggATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGACGCAGGCCAGGATGCGCAGCACGCGCAccc                                       >  2:1645666/1‑100 (MQ=255)
                                                           gATCACTCACCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGGGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTc                                      >  1:4209327/1‑101 (MQ=255)
                                                            aTCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCg                                     <  1:5784702/101‑1 (MQ=255)
                                                              cACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgc                                   >  1:6883293/1‑101 (MQ=255)
                                                              cACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgc                                   >  2:3875382/1‑101 (MQ=255)
                                                               aCTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgcg                                  >  2:5356435/1‑101 (MQ=255)
                                                                 tCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGGAGGCCAGGATGGGGAGCACGCGCACCGCGGCGcc                                >  1:2276624/1‑101 (MQ=255)
                                                                 tCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCGCGGCGcc                                >  2:4852863/1‑101 (MQ=255)
                                                                  cccccGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGccc                               >  1:219849/1‑101 (MQ=255)
                                                                   ccccGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCa                              >  1:3509257/1‑101 (MQ=255)
                                                                    cccGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAg                             <  1:7114292/101‑1 (MQ=255)
                                                                    cccGCCGCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAg                             <  2:175482/101‑1 (MQ=255)
                                                                     ccgccgCGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGa                            <  2:39138/101‑1 (MQ=255)
                                                                       gccgcGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAAc                          >  1:1412839/1‑101 (MQ=255)
                                                                        ccgcGTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAAcc                         <  2:3156998/101‑1 (MQ=255)
                                                                         cgcgTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCa                        <  2:5726364/101‑1 (MQ=255)
                                                                         cgcgTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCa                        <  2:3598789/101‑1 (MQ=255)
                                                                          gcgTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAg                       >  2:3350451/1‑101 (MQ=255)
                                                                          gcgTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAg                       >  1:517436/1‑101 (MQ=255)
                                                                           cgTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGc                      >  2:611793/1‑101 (MQ=255)
                                                                            gTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCCGAACCAGCg                     >  2:725663/1‑101 (MQ=255)
                                                                            gTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCg                     >  2:2744051/1‑101 (MQ=255)
                                                                            gTCGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCCCAGGCCAGGATGCGCACCACGCGCACCTCGGCGCCCAGAACCAGCg                     >  2:496315/1‑101 (MQ=255)
                                                                              cGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTc                   <  2:41325/101‑1 (MQ=255)
                                                                              cGATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTc                   <  2:3540616/101‑1 (MQ=255)
                                                                               gATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCa                  >  1:965296/1‑101 (MQ=255)
                                                                               gATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCa                  <  1:2426139/101‑1 (MQ=255)
                                                                               gATCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCa                  >  1:5579469/1‑101 (MQ=255)
                                                                                 tCAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGc                <  1:5557897/101‑1 (MQ=255)
                                                                                  cAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCa               >  2:7054539/1‑101 (MQ=255)
                                                                                  cAGCGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCa               <  1:589534/101‑1 (MQ=255)
                                                                                    gcgcGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACg             <  1:6841978/101‑1 (MQ=255)
                                                                                    gcgcGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACg             <  1:3350574/101‑1 (MQ=255)
                                                                                    gcgcGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACg             <  1:3193692/101‑1 (MQ=255)
                                                                                    gcgcGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCCCGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAAACAGGGTCAGCagc             >  2:5617989/1‑99 (MQ=255)
                                                                                     cgcgTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGt            <  2:6788267/101‑1 (MQ=255)
                                                                                      gcgTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTc           <  1:3036458/101‑1 (MQ=255)
                                                                                      gcgTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTc           >  1:1710468/1‑101 (MQ=255)
                                                                                      gcgTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTc           <  2:2968726/101‑1 (MQ=255)
                                                                                       cgTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCg          >  1:2054266/1‑101 (MQ=255)
                                                                                        gTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGc         <  2:4583826/101‑1 (MQ=255)
                                                                                        gTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGc         >  1:3451573/1‑101 (MQ=255)
                                                                                         tAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGcc        >  1:4629550/1‑101 (MQ=255)
                                                                                          agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       <  2:3088763/101‑1 (MQ=255)
                                                                                          agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       >  1:4984848/1‑101 (MQ=255)
                                                                                          agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       <  1:2020405/101‑1 (MQ=255)
                                                                                          agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       <  2:2347798/101‑1 (MQ=255)
                                                                                          agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       >  1:3957211/1‑101 (MQ=255)
                                                                                              cGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGc   >  2:1107314/1‑101 (MQ=255)
                                                                                               gTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGcc  >  2:6249468/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAGCGCGTAGAGCGTCACGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGCC  >  NC_014034/2982901‑2983096

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG