BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,048	C→A	11.0%	intergenic (+94/+82)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,048	0	C→A	11.0%	116.5 ‑3.5 ‑0.1	428	intergenic (+94/+82)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (18/29): ref base (210/171): total (228/200)
Fisher's exact test strand distribution p-value = 3.10e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.59e-01

Only 100 of 448 total aligned reads displayed.


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148


   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                    <  2:2832854/101‑1 (MQ=255)


   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                    <  1:1563450/101‑1 (MQ=255)


     gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGCATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGCCttcac                                                                                                  >  2:2168511/1‑98 (MQ=255)


       gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                                <  2:6852961/101‑1 (MQ=255)


        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                               <  2:5007126/101‑1 (MQ=255)


        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                               <  1:3789696/101‑1 (MQ=255)


        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                               <  1:1940840/101‑1 (MQ=255)


           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                            >  2:2330288/1‑101 (MQ=255)


            aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgc                                                                                           <  1:4781221/101‑1 (MQ=255)


               cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                        <  2:3552884/101‑1 (MQ=255)


                ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTGTGCCTTTCACCTTGGCGCaa                                                                                       >  1:4372538/1‑101 (MQ=255)


                   gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAata                                                                                    <  2:6273764/101‑1 (MQ=255)


                   gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAata                                                                                    <  1:4576460/101‑1 (MQ=255)


                    ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAActat                                                                                   >  2:2038998/1‑101 (MQ=255)


                    ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                   <  2:5458519/101‑1 (MQ=255)


                      cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                 <  1:240516/101‑1 (MQ=255)


                      cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                 >  1:5044593/1‑101 (MQ=255)


                        cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                               <  2:6689488/101‑1 (MQ=255)


                            cccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagcccg                                                                     >  2:5561885/1‑95 (MQ=37)


                             ccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                                                          <  1:817544/101‑1 (MQ=255)


                                  ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                                     >  2:6971103/1‑101 (MQ=255)


                                   ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                    >  2:1485824/1‑101 (MQ=255)


                                   ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                    <  2:7131601/101‑1 (MQ=255)


                                       ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                                >  1:4054798/1‑101 (MQ=255)


                                        cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                               >  1:3132267/1‑101 (MQ=255)


                                         tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                              <  2:5727587/101‑1 (MQ=255)


                                           aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                            >  1:2412216/1‑101 (MQ=255)


                                            cGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCTCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                           <  1:204489/101‑1 (MQ=255)


                                             ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGGCgg                                                          >  2:2963082/1‑101 (MQ=255)


                                             ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                          <  1:1080577/101‑1 (MQ=255)


                                              gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggg                                                         <  2:4577090/101‑1 (MQ=255)


                                                cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                       <  2:1004379/101‑1 (MQ=255)


                                                 aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                      <  2:1953809/101‑1 (MQ=255)


                                                  aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                     <  2:7040070/101‑1 (MQ=255)


                                                    tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                   >  1:125683/1‑101 (MQ=255)


                                                    tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                   <  2:1163569/101‑1 (MQ=255)


                                                      cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                 <  1:3629921/100‑3 (MQ=18)


                                                      cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                 <  1:5871399/100‑1 (MQ=255)


                                                      aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                 >  1:3690434/1‑101 (MQ=255)


                                                        cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cc                                              <  2:1692823/101‑1 (MQ=255)


                                                        cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cc                                              <  1:620020/101‑1 (MQ=255)


                                                         ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.ccc                                             <  2:3132154/101‑1 (MQ=255)


                                                          cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccc                                            <  2:2555744/101‑1 (MQ=255)


                                                           ccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccc                                           >  2:1103161/1‑101 (MQ=18)


                                                           ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccc                                           <  2:1027985/101‑1 (MQ=17)


                                                           ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccc                                           <  1:3231667/101‑1 (MQ=17)


                                                           ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.ccccc                                           <  2:2412122/101‑1 (MQ=255)


                                                            cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccc                                          >  1:1376174/1‑101 (MQ=255)


                                                            cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccc                                          >  2:1524438/1‑101 (MQ=255)


                                                             gggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccc                                         >  2:5957163/1‑100 (MQ=18)


                                                             gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                         <  1:2253918/101‑1 (MQ=255)


                                                             gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                         <  1:2820049/101‑1 (MQ=255)


                                                             gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                         <  1:3456237/101‑1 (MQ=255)


                                                             gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                         <  2:3910967/101‑1 (MQ=255)


                                                             gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGCGGGGCAGAg.cccccct                                         >  2:899902/1‑101 (MQ=255)


                                                               tatatGTGGCCCAAGGTGCAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGCCGGGGGGCAGCg.ccccccctc                                       <  2:2266105/100‑4 (MQ=18)


                                                               gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgc                                       <  2:3882021/101‑1 (MQ=255)


                                                               gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgc                                       >  2:5121118/1‑101 (MQ=255)


                                                                atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctct                                      <  1:6075578/101‑5 (MQ=17)


                                                                atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcc                                      >  1:4604443/1‑101 (MQ=255)


                                                                 tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctctt                                     <  1:4223528/101‑6 (MQ=17)


                                                                 tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctctt                                     <  1:927931/101‑6 (MQ=17)


                                                                 tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgccc                                     <  1:2171228/101‑1 (MQ=255)


                                                                   tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgccccc                                   >  1:6985503/1‑101 (MQ=255)


                                                                    ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccccc                                  >  1:2903665/1‑101 (MQ=255)


                                                                     tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccccct                                 <  2:2697226/101‑1 (MQ=255)


                                                                       gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                               <  1:6732480/101‑1 (MQ=255)


                                                                       gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                               <  1:2584308/101‑1 (MQ=255)


                                                                       gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                               >  2:4455576/1‑101 (MQ=255)


                                                                       gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                               >  2:4293255/1‑101 (MQ=255)


                                                                       gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                               >  2:3436881/1‑101 (MQ=255)


                                                                        aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTggg                              <  2:834675/101‑1 (MQ=255)


                                                                          cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGc                            <  1:6489102/101‑1 (MQ=255)


                                                                          cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGc                            >  2:5355387/1‑101 (MQ=255)


                                                                          cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGc                            >  1:5214322/1‑101 (MQ=255)


                                                                            aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCg                          >  1:753454/1‑101 (MQ=255)


                                                                              gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAg                        >  2:1843358/1‑101 (MQ=255)


                                                                               tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgc                       >  1:6685507/1‑101 (MQ=255)


                                                                                gacctcAG.ACCCCGTTTTGCCTTCCCCCTGGGCGAAAATACCCGGGGGGGAGCCCGGGGGGGGGGGGGGGCAGCC.CCCCCCTGCCCCCCTGGGGCCGAgcg                      <  2:2680105/96‑1 (MQ=255)


                                                                                gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcg                      >  1:2622012/1‑101 (MQ=255)


                                                                                gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcg                      >  2:4126998/1‑101 (MQ=255)


                                                                                 aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  2:1413688/1‑101 (MQ=255)


                                                                                 aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  1:1751257/1‑101 (MQ=255)


                                                                                 aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcgc                     <  2:6566969/101‑1 (MQ=255)


                                                                                    gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                  >  1:5728057/1‑101 (MQ=255)


                                                                                    agccggtCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGGTCCGCGGGGACGGGGGGCAGAGCCCCCCCTGCCCCCCTGGGGCCGAGCGCt                    <  2:5210950/95‑1 (MQ=25)


                                                                                     cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                 >  1:5644190/1‑101 (MQ=255)


                                                                                      aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:3215091/1‑101 (MQ=255)


                                                                                       g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  2:4691341/1‑101 (MQ=255)


                                                                                       g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  2:6456294/1‑101 (MQ=255)


                                                                                             cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGGGtt          >  2:1773580/1‑101 (MQ=255)


                                                                                              ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt         <  2:2525703/101‑1 (MQ=255)


                                                                                              ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt         >  1:3853313/1‑101 (MQ=255)


                                                                                               gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg        >  1:6940133/1‑101 (MQ=255)


                                                                                               gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCGCAGGCGTGTTTg        <  1:6400153/101‑1 (MQ=255)


                                                                                                tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       >  1:5973396/1‑101 (MQ=255)


                                                                                                tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       <  1:1794493/101‑1 (MQ=255)


                                                                                                tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       >  1:5477782/1‑101 (MQ=255)


                                                                                                tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       >  1:3384482/1‑101 (MQ=255)


                                                                                                  tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     <  1:1137046/101‑1 (MQ=255)


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG