Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	69.2	18	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (0/18):  ref base (0/0):  total (0/18)

GCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGG  >  NC_014034/3223838‑3223952                                                                                                  |                  gcgcCGTCCCAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggg                <  2:5395145/101‑1 (MQ=255) gcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggg                <  1:2140190/101‑1 (MQ=255)  cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg               >  1:5474756/1‑101 (MQ=255)    ccggtccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc             <  1:2317055/96‑1 (MQ=255)       tCCCAGCCCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc          <  1:1749472/101‑1 (MQ=255)       tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc          <  1:3183998/101‑1 (MQ=255)       tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc          <  2:3915674/101‑1 (MQ=255)       ccccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc          <  2:5598699/100‑1 (MQ=255)        cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca         <  1:6153431/101‑2 (MQ=255)          cAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag       <  1:4929619/101‑4 (MQ=255)          cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTTCACGGCCCGCAAGGCCGCAAGCGCACCATCAGCCAggaggccggggcacccaaa       >  2:3336387/1‑93 (MQ=255)          cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag       <  1:7041792/101‑4 (MQ=255)            gacccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcg     <  1:3750433/98‑6 (MQ=255)            gAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcg     <  2:1632285/101‑6 (MQ=255)            gAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcg     >  1:2667172/1‑96 (MQ=255)             aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCCCCATCGGCCAggggggggggggggccaaccgc    >  1:633386/1‑91 (MQ=255)             aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgc    <  1:6058333/101‑7 (MQ=255)              aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc   <  2:2019510/101‑8 (MQ=255)              aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc   <  2:2227977/101‑8 (MQ=255)              aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc   <  2:4712833/101‑8 (MQ=255)               ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca  <  2:1812742/101‑9 (MQ=255)               ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca  <  2:5070831/101‑9 (MQ=255)               ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca  <  2:6506233/101‑9 (MQ=255)               ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca  <  2:90185/101‑9 (MQ=255)                                                                                                  |                  GCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGG  >  NC_014034/3223838‑3223952 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG