Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	222.4	59	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (1/58):  ref base (0/0):  total (1/58)

CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591                                                                                                   |                                                                                                  cggtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCgcgggggg                                                                                                 <  2:2582082/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCgcgggggggc                                                                                               >  1:3408567/1‑99 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:6794909/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:6726050/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  2:3414182/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:3008729/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:318185/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:3793991/101‑1 (MQ=255)   gtgccggtgcGCGCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCGGGTCGACCCGCGGGGGGCt                                                                                               <  2:2657553/101‑1 (MQ=255)    tgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTc                                                                                              <  2:1356533/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  2:1724332/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             >  1:5737071/1‑101 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  2:4222785/101‑1 (MQ=255)      ccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCg                                                                                            <  1:1314553/101‑1 (MQ=255)       ctgtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGc                                                                                           <  1:5867702/99‑1 (MQ=255)       cggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGTCCCGCGGGGGGCTCCGc                                                                                           <  2:5736769/101‑1 (MQ=255)       cggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGc                                                                                           <  1:12395/101‑1 (MQ=255)        ggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcc                                                                                          <  1:3042742/101‑1 (MQ=255)        ggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcc                                                                                          <  2:614205/101‑1 (MQ=255)        ggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcc                                                                                          <  1:1336430/101‑1 (MQ=255)         gtgcTGTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  2:3300424/101‑1 (MQ=255)         gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  2:4841102/101‑1 (MQ=255)         gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  1:2030153/101‑1 (MQ=255)         gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcag                                                                                         >  2:5867400/1‑99 (MQ=255)          tgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  1:5498551/101‑1 (MQ=255)          tgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  1:4500608/101‑1 (MQ=255)          tgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  1:2648789/101‑1 (MQ=255)          tgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  2:68754/101‑1 (MQ=255)           gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGCCGACCCGCGGGGGGCTCCGccccc                                                                                       <  2:3408443/101‑1 (MQ=255)            ctctGGCCCGAGGACGAGGCGCGGCTGGAAGACCTGCTGAAGCACTAGGGATTTCGCCCGGGGTTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccct                                                                                      <  1:454181/101‑2 (MQ=37)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  2:3417549/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  2:704068/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  1:752952/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  1:493780/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  1:5004890/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  1:454180/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCGGAAGGACTAGGGATTTCCCCCGGGGCTCGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  2:101541/101‑1 (MQ=39)              ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  2:1178345/101‑1 (MQ=255)               tcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCa                                                                                   <  1:1866548/101‑1 (MQ=255)                cgtgcGAGGAAGAGGCGCGGCTGCAAGACCTGCTGAAGGCCTAGGGCTCTCGCCCGGGGCTTGCGCCCCGGGAGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  2:70187/97‑1 (MQ=37)                cGGCCGAGGAGGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  2:3686768/101‑1 (MQ=255)                cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  2:5724428/101‑1 (MQ=255)                   ccGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  1:3885608/101‑1 (MQ=255)                    cGAGGAATCGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  2:4627464/101‑1 (MQ=255)                    cGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGTCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  2:3081896/101‑1 (MQ=255)                    cGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  1:6860216/101‑1 (MQ=255)                         aaGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  2:6283777/101‑1 (MQ=255)                                gcgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAtttt                                                                  <  2:1407189/101‑1 (MQ=255)                                gcgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAtttt                                                                  <  1:2025371/101‑1 (MQ=255)                                  gtttGAAAGACCTGCTGAAGTTCTAGGGATTTCTCCTGGTGCTGGCGCCCCGGGCTACCCGCGGGGGGCTCCGCCCCCCTCACCCCCCGGGCGTATTTTGc                                                                <  1:3556683/98‑1 (MQ=21)                                    cTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCa                                                              <  2:98659/101‑1 (MQ=255)                                        aaGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGa                                                          <  1:6248684/101‑1 (MQ=255)                                          gACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGaaa                                                        <  2:697977/101‑1 (MQ=255)                                            ccTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAg                                                      <  1:5231291/101‑1 (MQ=255)                                                      gACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTg                                            <  1:7082400/101‑1 (MQ=255)                                                      gACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTg                                            <  1:2859966/101‑1 (MQ=255)                                                         tAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTc                                         <  1:5040790/101‑1 (MQ=255)                                                           gggATTCCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGt                                       <  1:5040222/101‑1 (MQ=255)                                                                tgcGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGt                                  <  2:481393/99‑1 (MQ=255)                                                                  cGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGttt                                <  1:4438128/101‑1 (MQ=255)                                                                       ggggCCTGCGCCCCGGGCAACCCGCGGGGCGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGttt                           <  2:3176847/101‑1 (MQ=25)                                                                          gCTGGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGc                        <  1:5725840/101‑1 (MQ=255)                                                                             tGCGCCCCGGGCGGCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCttt                     <  1:5492203/101‑1 (MQ=255)                                                                                    cGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTTGTCGTGACGTTTGGTTTCGCTTTGCCTTTc              <  2:2336423/101‑1 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  2:2776928/1‑101 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  2:6695572/1‑101 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  2:4691944/1‑101 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgac  >  2:1176690/1‑99 (MQ=255)                                                                                                   |                                                                                                  CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG