Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,957 C→A 1.5% intergenic (+78/‑293) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9570C→A 1.5% 3.9 ‑1.6 ‑0.2341intergenic (+78/‑293)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (176/160):  total (177/164)
Fisher's exact test strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.84e-01

Only 100 of 368 total aligned reads displayed.

GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGC  >  NC_014034/580862‑581057
                                                                                                |                                                                                                     
   ttCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGcc                                                                                                >  2:2705941/1‑101 (MQ=255)
    tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                               <  2:1907466/101‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                              >  1:6187075/1‑101 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                              <  2:6818528/101‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                              >  2:4784664/1‑101 (MQ=255)
      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                             <  2:5952217/101‑1 (MQ=255)
      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                             <  2:2554565/101‑1 (MQ=255)
       gCCTCGATTGAGGCGGCCCGGGCAGGGGGGGCTGCCCCCTCTTTCGGCTGCGCCGAAAATCACCCCCGGGGGATTTGAGGCAAGATAAAAGGGCGCCTTT.c                                                                                           >  1:21046/1‑101 (MQ=255)
       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.c                                                                                           <  1:3672500/101‑1 (MQ=255)
         cTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CAc                                                                                         <  1:424205/101‑1 (MQ=255)
          tCGATTGGGGCGGCCCGGGCAGGGGGCGGTGCCCCCCCTTTCGGCTGCGCCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTAT.CAcc                                                                                        >  1:3905906/1‑101 (MQ=37)
           cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                                       <  1:5835862/101‑1 (MQ=255)
           cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                                       <  2:513213/101‑1 (MQ=255)
            gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCtt                                                                                      >  1:2771500/1‑101 (MQ=255)
             aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTg                                                                                     >  1:7067734/1‑101 (MQ=255)
               tGAGGCGGCCCGGGCAGGGGGCGACGCCCCCTCTTTCGGCTGAGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGt                                                                                   >  2:1642934/1‑101 (MQ=25)
                  ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCa                                                                                <  2:6442237/101‑1 (MQ=255)
                   gcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCaa                                                                               <  2:3822887/101‑1 (MQ=255)
                    cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGGAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCaaa                                                                              >  2:2850191/1‑101 (MQ=255)
                    cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCaaa                                                                              <  1:6000963/101‑1 (MQ=255)
                    cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCaaa                                                                              <  1:5293613/101‑1 (MQ=255)
                     ggcCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTTACCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTAT.GACCTTTGTGCaaaa                                                                             >  2:5606578/1‑100 (MQ=21)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCCGCGCCGGAATTCACCCCCCGGGTATTTTAGGCAAGATAAACGGGCGCCGATCCACCTTGGGCCaaa                                                                              >  2:2390877/1‑101 (MQ=12)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAat                                                                             <  1:6883253/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAatat                                                                           <  2:3778492/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAatat                                                                           <  2:446895/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.AACCTTGGTGCAAatat                                                                           >  1:6571676/1‑101 (MQ=255)
                        ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATc                                                                          <  1:239339/101‑1 (MQ=255)
                         cgtgcggggggCGCTTCCCCCTCTTTCGGCTTCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATcc                                                                         <  2:5401593/95‑1 (MQ=21)
                          gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCg                                                                        <  1:852751/101‑1 (MQ=255)
                             cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggg                                                                     >  2:6537501/1‑101 (MQ=255)
                             cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggg                                                                     >  2:5778704/1‑101 (MQ=255)
                              aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggg                                                                    <  2:7001257/101‑1 (MQ=255)
                               gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggggg                                                                   >  2:4257454/1‑101 (MQ=255)
                                 gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                                 <  1:5738909/101‑1 (MQ=255)
                                 gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                                 <  2:7088240/101‑1 (MQ=255)
                                  ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGa                                                                <  1:152622/101‑1 (MQ=255)
                                   gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAg                                                               >  2:5879302/1‑101 (MQ=255)
                                    cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTGT.CACCTTGGTGCAAATATCCGGGGGGTGAgg                                                              <  1:4664209/101‑1 (MQ=255)
                                     gcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                             >  1:2706571/1‑101 (MQ=255)
                                       tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                           >  2:6698932/1‑101 (MQ=255)
                                           cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                       >  2:533432/1‑101 (MQ=255)
                                           cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                       >  1:737834/1‑101 (MQ=255)
                                           cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                       <  1:4845328/101‑1 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                    <  1:5417353/101‑1 (MQ=255)
                                               ctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGCAAGCCGa                                                   >  2:2355034/1‑101 (MQ=255)
                                                tttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                  >  2:1947988/1‑101 (MQ=255)
                                                 ttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.TACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                 <  2:3461487/101‑1 (MQ=255)
                                                 ttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                 >  2:6659112/1‑101 (MQ=255)
                                                  tCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggg                                                >  1:5502358/1‑101 (MQ=255)
                                                     gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                             >  1:7018627/1‑101 (MQ=255)
                                                        gcgcCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CCCCTTGGTGCAAATACCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                          <  2:6060511/101‑1 (MQ=38)
                                                        gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                          <  1:3734904/101‑1 (MQ=255)
                                                         cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                         <  1:622198/101‑1 (MQ=255)
                                                          gcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcgc                                        >  2:4112711/1‑101 (MQ=255)
                                                            tgAAATTCACCTCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                      <  2:3332408/100‑1 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                     >  2:181340/1‑101 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                     >  2:2171349/1‑101 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                     >  1:4880069/1‑101 (MQ=255)
                                                              aaaTTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                    <  1:350837/101‑1 (MQ=255)
                                                              aaaTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                    >  2:2608505/1‑101 (MQ=255)
                                                                aTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGGCGAGGGGGCAGCGCCCCCtt                                  >  1:5593648/1‑101 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                >  1:5688527/1‑101 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                <  1:1656683/101‑1 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                <  1:3154273/101‑1 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                               >  1:6455480/1‑101 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                               >  2:1280410/1‑101 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGGGGGGCCCGCGCCCCCTTTTc                               >  2:2291003/1‑101 (MQ=37)
                                                                    aCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                              >  2:650343/1‑101 (MQ=255)
                                                                      cccgGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                            <  1:2153540/101‑1 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgt                           <  2:5246574/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACTTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                          <  2:6950961/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                        >  2:3569573/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                        >  1:4540545/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                        >  1:96897/1‑101 (MQ=255)
                                                                           ggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                       >  1:1301125/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgg                      >  2:1040747/1‑100 (MQ=255)
                                                                             atatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                     >  1:3823803/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                     <  2:760988/101‑1 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                    <  2:6186756/101‑1 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                    <  2:5073985/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTggt                   >  1:3869592/1‑100 (MQ=255)
                                                                               atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                   >  1:5212464/1‑101 (MQ=255)
                                                                               atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                   >  2:149602/1‑101 (MQ=255)
                                                                                tttGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                  <  1:1841456/101‑1 (MQ=255)
                                                                                tttGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                  <  1:171893/101‑1 (MQ=255)
                                                                                 ttGAGGCAATATAAACGGGCGCCTAT.CGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                 <  2:866112/101‑1 (MQ=37)
                                                                                  tGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                >  1:1137151/1‑101 (MQ=255)
                                                                                   gAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc               >  2:2968722/1‑101 (MQ=255)
                                                                                        aaGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGGGGATCGCGCAAc          <  1:5052555/101‑1 (MQ=255)
                                                                                         agataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg         <  1:6700663/96‑1 (MQ=255)
                                                                                          gataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt        <  1:3419831/97‑1 (MQ=255)
                                                                                           ataaaCGGGCGCCTAT.CACCTTGGTGCAAATATTCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt       >  2:3848086/4‑101 (MQ=255)
                                                                                           ataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt       >  2:4524540/4‑101 (MQ=255)
                                                                                           ataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt       <  2:2715483/98‑1 (MQ=255)
                                                                                            taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      <  2:31874/99‑1 (MQ=255)
                                                                                            taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      <  1:331282/99‑1 (MQ=255)
                                                                                             aaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa     >  2:2115732/2‑101 (MQ=255)
                                                                                             aaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa     <  1:4467587/100‑1 (MQ=255)
                                                                                               aCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg   <  1:4112905/101‑1 (MQ=255)
                                                                                                |                                                                                                     
GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGC  >  NC_014034/580862‑581057

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG