Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,975 G→C 1.3% intergenic (+96/‑275) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9750G→C 1.3% 2.3 ‑1.7 ‑0.5379intergenic (+96/‑275)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (200/174):  total (201/178)
Fisher's exact test strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.14e-01

Only 100 of 400 total aligned reads displayed.

TTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.AT.AA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGC  >  NC_014034/580876‑581075
                                                                                                        |                                                                                                    
 tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGt                                                                                                     >  1:1233927/1‑101 (MQ=255)
  gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGGAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTg                                                                                                    >  2:6980977/1‑101 (MQ=255)
  gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTgggg                                                                                                    >  2:3803798/1‑99 (MQ=255)
  gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTg                                                                                                    >  2:5186145/1‑101 (MQ=255)
     gcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCaa                                                                                                 <  1:6656496/101‑1 (MQ=255)
       ggcCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTTA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGGGCCTAT.GACCTTTGTGCaaaa                                                                                               >  2:5606578/1‑100 (MQ=21)
       ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCCGCGCCGGAATTCA.CCCCCCGGGTATTTTAGGCAAG.ATAAA.CGGGCGCCGATCCACCTTGGGCCaaa                                                                                                >  2:2390877/1‑101 (MQ=12)
        gcCCGGGCAGGGGGCGCCTCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAata                                                                                              <  1:6923490/101‑1 (MQ=25)
          ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATc                                                                                            <  2:3031470/101‑1 (MQ=255)
           cgtgcggggggCGCTTCCCCCTCTTTCGGCTTCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATcc                                                                                           <  2:5401593/95‑1 (MQ=21)
            gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCg                                                                                          <  1:852751/101‑1 (MQ=255)
             ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCgg                                                                                         <  2:6859292/101‑1 (MQ=255)
              gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCggg                                                                                        >  2:3220216/1‑101 (MQ=255)
               cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCgggg                                                                                       >  2:5778704/1‑101 (MQ=255)
                aGGGGGCGCTGCGCCCTCTTTCGGCTGCGCCGAAATTCA.CCGCCCCGATACTTGAGGCAAGTAA.AA.CGGGCGCCTAT.CGTCTTGATGCGAATATCCggggg                                                                                      >  1:7074644/1‑101 (MQ=255)
                  ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCggggggg                                                                                    >  2:6021891/1‑100 (MQ=255)
                   gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                                                   <  1:5738909/101‑1 (MQ=255)
                     gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAg                                                                                 <  1:1535487/101‑1 (MQ=255)
                      cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTGT.CACCTTGGTGCAAATATCCGGGGGGTGAgg                                                                                <  1:4664209/101‑1 (MQ=255)
                      cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAgg                                                                                <  2:6687152/101‑1 (MQ=255)
                       gcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                                               >  1:2706571/1‑101 (MQ=255)
                          gCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                                            >  1:4790972/1‑101 (MQ=255)
                            ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                          >  2:350093/1‑101 (MQ=255)
                            ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                          <  1:1696471/101‑1 (MQ=255)
                               ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                                                       <  2:4279595/101‑1 (MQ=255)
                                tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                      <  2:6617835/101‑1 (MQ=255)
                                tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                      <  2:1911457/101‑1 (MQ=255)
                                tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                      <  2:3722473/101‑1 (MQ=255)
                                  tttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                                    <  2:4605267/101‑1 (MQ=255)
                                    tCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggg                                                                  >  1:5502358/1‑101 (MQ=255)
                                    tCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggg                                                                  >  1:764882/1‑101 (MQ=255)
                                       gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGCGGTGAGGCCGAAAGCCGAGGGGGc                                                               <  2:3404812/101‑1 (MQ=255)
                                        cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                              >  2:2332169/1‑101 (MQ=255)
                                         tGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                                             <  1:342727/101‑1 (MQ=255)
                                         tGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                                             <  1:3904287/101‑1 (MQ=255)
                                         cttcggccaaTTCACCCCCCGGGATATTTGCGGCAAG.GTGAA.AGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgc                                                            <  1:2145794/93‑1 (MQ=37)
                                          ttcggccaaTTCACCCCCCGGGATATTTGCGGCAAG.GTGAA.AGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg                                                           <  1:2202565/94‑1 (MQ=37)
                                          gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                                            <  1:3734904/101‑1 (MQ=255)
                                           tcggccaaTTCACCCCCCGGGATATTTGCGGCAAG.GTGAA.AGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                                          <  2:6141742/95‑1 (MQ=37)
                                           cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                                           >  2:2697472/1‑101 (MQ=255)
                                              cGAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                                        <  2:2884903/101‑1 (MQ=255)
                                               gAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                       >  1:4880069/1‑101 (MQ=255)
                                               gAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                       <  2:3833563/101‑1 (MQ=255)
                                               gAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                       >  2:2171349/1‑101 (MQ=255)
                                               gAAATTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                       >  2:181340/1‑101 (MQ=255)
                                                aaaTTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                      <  2:1973558/101‑1 (MQ=255)
                                                 aaTTCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                     <  2:776984/101‑1 (MQ=255)
                                                    tCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                                  <  2:2744305/101‑1 (MQ=255)
                                                    tCA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                                  >  1:5688527/1‑101 (MQ=255)
                                                     cA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                                 <  1:6231219/101‑1 (MQ=255)
                                                     cA.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATACGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                                 >  2:5256997/1‑101 (MQ=255)
                                                      a.CCCCCGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                >  2:650343/1‑101 (MQ=255)
                                                       ccccccGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                <  2:6412295/100‑1 (MQ=255)
                                                        cccccGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTccc                                               <  2:2569389/101‑1 (MQ=255)
                                                          cccGGGATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgt                                             <  1:533444/101‑1 (MQ=255)
                                                             gggATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                          >  1:96897/1‑101 (MQ=255)
                                                              ggATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                         >  1:2402861/1‑101 (MQ=255)
                                                               gagatTTGAGGCAAG.AA.AATCGGGCGCCTAT.CCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                        <  2:7074270/98‑1 (MQ=37)
                                                               gATATTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgg                                        >  2:1040747/1‑100 (MQ=255)
                                                                atatTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                                       >  1:3823803/1‑101 (MQ=255)
                                                                 tatTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                      <  1:3220332/101‑1 (MQ=255)
                                                                  atTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                     >  2:3360638/1‑101 (MQ=255)
                                                                  atTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                     >  2:149602/1‑101 (MQ=255)
                                                                  atTTGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                     >  1:5212464/1‑101 (MQ=255)
                                                                   tttGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                    >  1:583624/1‑101 (MQ=255)
                                                                    ttGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                   <  1:3528492/101‑1 (MQ=255)
                                                                    ttGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                   <  2:4175217/101‑1 (MQ=255)
                                                                     tGAGGCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                  >  1:1137151/1‑101 (MQ=255)
                                                                         gCAAG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa                              >  2:1255616/1‑101 (MQ=255)
                                                                           aaG.ATAAA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGGGGATCGCGCAAc                            <  1:5052555/101‑1 (MQ=255)
                                                                              gataaa.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt                          >  1:2845930/5‑101 (MQ=255)
                                                                                taaa.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc                        <  2:3905731/99‑1 (MQ=255)
                                                                                 aaa.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa                       >  2:2115732/2‑101 (MQ=255)
                                                                                  aa.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                      >  1:5391062/1‑101 (MQ=255)
                                                                                   a.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAGGTTCAgg                     >  2:5091217/1‑101 (MQ=255)
                                                                                   a.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                     <  2:725349/101‑1 (MQ=255)
                                                                                   a.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                     <  1:4112905/101‑1 (MQ=255)
                                                                                     cGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGc                    <  1:4486703/101‑1 (MQ=255)
                                                                                      gggCGCCTAT.CAACTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg                   >  2:1367999/1‑101 (MQ=255)
                                                                                          gcCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtt               <  1:4524779/101‑1 (MQ=255)
                                                                                           ccTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCGACGTTCAGGCGGAttt              >  1:6033138/1‑101 (MQ=255)
                                                                                           ccTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt              <  1:1780864/101‑1 (MQ=255)
                                                                                           ccTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt              <  2:228530/101‑1 (MQ=255)
                                                                                           ccTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt              >  1:673324/1‑101 (MQ=255)
                                                                                            cTAT.CACCTTGGTGCCAATAGCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt             <  1:5606868/101‑1 (MQ=255)
                                                                                            cTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt             <  1:1911999/101‑1 (MQ=255)
                                                                                            cTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt             >  1:6488046/1‑101 (MQ=255)
                                                                                            cTAT.CACCTTGGTGCAAATACCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtgtt             >  2:6803233/1‑101 (MQ=255)
                                                                                             tAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc            >  2:5932973/1‑101 (MQ=255)
                                                                                              aT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct           >  1:6204232/1‑101 (MQ=255)
                                                                                              aT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct           >  2:6320144/1‑101 (MQ=255)
                                                                                                 ttccTTGGTGCAAAAGGACTGGGTGTGAGTCCGAAAGCCGAGGGGGCAGCGCGCCCTTTTCCCTTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc         <  2:880737/99‑1 (MQ=255)
                                                                                                 cACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc         >  1:1432981/1‑101 (MQ=255)
                                                                                                  aCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTccc        >  1:3324939/1‑101 (MQ=255)
                                                                                                  aCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTccc        <  2:4879825/101‑1 (MQ=255)
                                                                                                   ccTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt       <  1:4217103/101‑1 (MQ=255)
                                                                                                   ccTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt       >  2:4491260/1‑101 (MQ=255)
                                                                                                    cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg      <  1:5887627/101‑1 (MQ=255)
                                                                                                    cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg      >  2:2507830/1‑101 (MQ=255)
                                                                                                        gTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGc  >  1:6424638/1‑101 (MQ=255)
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TTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAG.AT.AA.CGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGC  >  NC_014034/580876‑581075

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG