Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 625,791 G→C 100% R533R (CGG→CGC anfA → nitrogen fixation regulatory protein AnfA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034625,7910G→C100.0% 1229.3 332R533R (CGG→CGCanfAnitrogen fixation regulatory protein AnfA
Reads supporting (aligned to +/- strand):  new base (151/181):  ref base (0/0):  total (151/181)

Only 100 of 337 total aligned reads displayed.

ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888
                                                                                                  |                                                                                                 
acgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTa                                                                                                 <  1:1950364/101‑1 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                >  1:3051113/1‑101 (MQ=255)
  gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               >  1:4395267/1‑101 (MQ=255)
  gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               >  1:419406/1‑101 (MQ=255)
  gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               >  1:405177/1‑101 (MQ=255)
  gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               <  2:4159788/101‑1 (MQ=255)
      cAGGACAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCgcgc                                                                                           >  1:3668141/1‑101 (MQ=255)
       aGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCt                                                                                          <  2:3203086/101‑1 (MQ=255)
        gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                         <  1:2944750/101‑1 (MQ=255)
         ggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTc                                                                                        <  2:3631512/101‑1 (MQ=255)
            aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                     >  2:5232049/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                    >  1:4495458/1‑101 (MQ=255)
              tatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCCCCGCTATCGCGCTTCGGCCt                                                                                   >  2:5728573/1‑101 (MQ=255)
               atCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTg                                                                                  <  2:6343036/101‑1 (MQ=255)
               atCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTg                                                                                  >  2:2452948/1‑101 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                >  1:6798369/1‑101 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                <  1:2269411/101‑1 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                <  2:1580174/101‑1 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                               >  1:4910627/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                               <  1:736909/101‑1 (MQ=255)
                   gCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCg                                                                              <  1:4365359/101‑1 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                            <  2:297054/101‑1 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                            >  2:1386277/1‑101 (MQ=255)
                     cAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGAc                                                                            <  1:1786592/101‑1 (MQ=255)
                      aGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                           <  1:5122409/101‑1 (MQ=255)
                         cggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGcc                                                                        <  2:4344926/101‑1 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                       <  1:5274308/101‑1 (MQ=255)
                           gcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCaa                                                                      <  1:6255783/101‑1 (MQ=255)
                            ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACGCGCACCGCTATCGCGCTTCGGCCTGATCGACCGCCAAt                                                                     >  2:4752934/1‑101 (MQ=255)
                            ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     >  1:4554705/1‑101 (MQ=255)
                            ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     >  2:1642296/1‑101 (MQ=255)
                            ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     >  1:6971751/1‑101 (MQ=255)
                            ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     >  1:1729330/1‑101 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGGCTGATCGACGGGCAATg                                                                    >  2:5916984/1‑101 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTGCGGCCTGATCGACGGCCCATg                                                                    >  2:3309238/1‑101 (MQ=255)
                               aGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAc                                                                  <  2:2096693/101‑1 (MQ=255)
                                 cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                <  1:5713598/101‑1 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                               >  1:3496839/1‑101 (MQ=255)
                                   tttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGaa                                                              >  1:4302195/1‑101 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                            >  1:2651958/1‑101 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          <  1:5826950/101‑1 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          <  2:3667992/101‑1 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          <  2:7081567/101‑1 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          <  2:6702775/101‑1 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          >  1:521045/1‑101 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          <  2:6063535/101‑1 (MQ=255)
                                        aGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCg                                                         >  2:1909274/1‑101 (MQ=255)
                                             tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                    <  1:4672313/101‑1 (MQ=255)
                                             tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                    <  2:4452864/101‑1 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGccccc                                                   >  2:3884593/1‑100 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                   >  1:3786960/1‑101 (MQ=255)
                                              cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                   >  1:889294/1‑101 (MQ=255)
                                                cgcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAGCGGCCCCGCt                                                 <  1:964549/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               <  2:4634767/101‑1 (MQ=255)
                                                  ccGGGTGCCGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               <  1:1160814/101‑1 (MQ=255)
                                                   cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                              <  2:3772708/101‑1 (MQ=255)
                                                    gggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcgg                                             >  2:492463/1‑101 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                       >  2:6810231/1‑101 (MQ=255)
                                                           gggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCt                                      >  2:5282011/1‑101 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                  >  2:1075724/1‑101 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                  <  2:3496703/101‑1 (MQ=255)
                                                                tGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTc                                 <  2:20122/101‑1 (MQ=255)
                                                                tGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTc                                 <  2:4222654/101‑1 (MQ=255)
                                                                tGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTc                                 <  1:6427329/101‑1 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCt                                <  1:1900985/101‑1 (MQ=255)
                                                                  gggATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  1:3201537/100‑1 (MQ=255)
                                                                  cGGATGGGGCGTCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  2:2804694/101‑1 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               >  2:5381457/1‑101 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  2:6228392/101‑1 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  2:868349/101‑1 (MQ=255)
                                                                    gATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTg                             <  1:6684615/101‑1 (MQ=255)
                                                                    gATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTg                             >  1:5582330/1‑101 (MQ=255)
                                                                    gATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCCTTTTCTTTg                             >  2:5590256/1‑101 (MQ=255)
                                                                     gtGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGc                            <  1:1611803/100‑1 (MQ=255)
                                                                       ggggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTTCgg                          >  1:5923804/1‑101 (MQ=255)
                                                                       ggggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCgg                          >  2:452793/1‑101 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGc                         <  1:5460625/101‑1 (MQ=255)
                                                                          gcggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCCTTTTCTTTGCGGCAg                       >  1:6085909/1‑101 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAgg                      >  1:4948408/1‑100 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGc                      >  1:2868781/1‑101 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGc                      >  1:5358604/1‑101 (MQ=255)
                                                                           cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGc                      <  2:2651862/101‑1 (MQ=255)
                                                                            ggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAggc                     >  2:1797540/1‑99 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  1:1075759/101‑1 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  1:1595002/101‑1 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  2:470212/101‑1 (MQ=255)
                                                                                 ggCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccccgtc                >  1:2607203/1‑97 (MQ=255)
                                                                                  gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCg               <  2:5123392/101‑1 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCgg              <  1:5150021/101‑1 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGCTCgg              >  2:4267168/1‑101 (MQ=255)
                                                                                    aTCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCCGGCCCTTCggg             >  1:6291676/1‑100 (MQ=255)
                                                                                    aTCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGa             <  1:6719606/101‑1 (MQ=255)
                                                                                      cGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAAt           <  1:141746/101‑1 (MQ=255)
                                                                                      cGACCCGCACCACTAACGCGCTTCGGACTGATCGACGGCAAATCACCGAATTCCGGCCACGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAAt           <  2:6799589/101‑1 (MQ=255)
                                                                                       gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAAtt          >  1:2325715/1‑101 (MQ=255)
                                                                                        aCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTc         <  2:5785922/101‑1 (MQ=255)
                                                                                            gCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGtt     <  2:4002680/101‑1 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTTGGAATTTCGttt    >  1:6509420/1‑101 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt    <  2:2247790/101‑1 (MQ=255)
                                                                                               ccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAc  <  2:6598912/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG