Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,120 G→C 41.2% V829V (GTC→GTG RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,1200G→C41.2% 159.5 ‑1.0 ‑0.2131V829V (GTC→GTGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (25/29):  ref base (42/35):  total (67/64)
Fisher's exact test strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.34e-01

Only 100 of 149 total aligned reads displayed.

GCCGGGTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGG  >  NC_014034/681020‑681217
                                                                                                    |                                                                                                 
gCCGGGTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGcgcg                                                                                                   <  1:1753359/101‑1 (MQ=255)
  cGGGTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGcgac                                                                                                 >  1:4974830/1‑101 (MQ=255)
   gggTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGcgacg                                                                                                <  2:6261412/101‑1 (MQ=255)
    ggTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGcgacga                                                                                               >  1:7070549/1‑101 (MQ=255)
     gTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGGCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGAt                                                                                              <  1:2620970/101‑1 (MQ=255)
     gTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGAt                                                                                              <  1:4342424/101‑1 (MQ=255)
     gTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGAt                                                                                              <  2:652394/101‑1 (MQ=255)
     gTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGAt                                                                                              <  2:3219441/101‑1 (MQ=255)
       cAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGt                                                                                            <  1:2325246/101‑1 (MQ=255)
       cAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGt                                                                                            >  2:5618854/1‑101 (MQ=255)
       cAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGt                                                                                            >  2:5527384/1‑101 (MQ=255)
       cAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGt                                                                                            <  1:2901946/101‑1 (MQ=255)
        aGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTc                                                                                           <  1:6125964/101‑1 (MQ=255)
        aGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTc                                                                                           <  2:22072/101‑1 (MQ=255)
        aGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTc                                                                                           <  1:366459/101‑1 (MQ=255)
        aGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTc                                                                                           <  2:4089894/101‑1 (MQ=255)
         gATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCGGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcg                                                                                          <  2:2760004/101‑2 (MQ=255)
         gATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcg                                                                                          >  1:1775780/1‑100 (MQ=255)
         gATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcg                                                                                          >  2:6520286/1‑100 (MQ=255)
          aTCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcgc                                                                                         >  1:5561588/1‑99 (MQ=255)
          aTCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGACATCGCAGCCATCTGATCGCCGGTAAGCGCGACGATGTcgc                                                                                         >  2:6667676/1‑99 (MQ=255)
           tCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcgcc                                                                                        <  1:3946057/101‑4 (MQ=255)
           tCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcgcc                                                                                        <  2:1307318/101‑4 (MQ=255)
            cctcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcgccg                                                                                       >  1:6219499/1‑97 (MQ=255)
             ctcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTcgccgg                                                                                      <  2:3475109/101‑6 (MQ=255)
              tcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGt                                                                                     >  2:3943452/1‑101 (MQ=255)
              tcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGt                                                                                     >  2:5846454/1‑101 (MQ=255)
              tcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGACCGCCGGTCAGCGCGACGATGTCGCCGGt                                                                                     >  1:4970143/1‑101 (MQ=255)
               cgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtc                                                                                    <  1:4396184/101‑2 (MQ=255)
                ggtctcgtTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGATGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATCTCGCCGGtcg                                                                                   <  2:6849546/98‑3 (MQ=38)
                gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                   >  1:6583747/1‑99 (MQ=255)
                gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                   >  1:4339879/1‑99 (MQ=255)
                gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                   <  1:961079/101‑3 (MQ=255)
                 acctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcga                                                                                  >  2:1846690/1‑98 (MQ=255)
                 acctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcga                                                                                  >  1:139276/1‑98 (MQ=255)
                 acctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcga                                                                                  >  1:1974033/1‑98 (MQ=255)
                 acctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcga                                                                                  >  1:5547483/1‑98 (MQ=255)
                  cctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgac                                                                                 <  1:288788/101‑5 (MQ=255)
                   ctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgaca                                                                                >  1:5308683/1‑96 (MQ=255)
                   ctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgaca                                                                                <  2:4160531/101‑6 (MQ=255)
                    tcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacag                                                                               <  1:3164621/101‑7 (MQ=38)
                    tcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacag                                                                               >  2:755956/1‑95 (MQ=38)
                     cgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCGCCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagc                                                                              >  1:6409673/1‑94 (MQ=38)
                     cgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagc                                                                              <  2:1741374/101‑8 (MQ=38)
                      gaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagcc                                                                             >  2:6112211/1‑93 (MQ=37)
                      gaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagcc                                                                             >  2:4135888/1‑93 (MQ=37)
                      gaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagcc                                                                             >  2:5150765/1‑93 (MQ=37)
                       aTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagccc                                                                            >  2:2971945/1‑92 (MQ=37)
                       aTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagccc                                                                            >  1:1464663/1‑92 (MQ=37)
                                aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                   >  1:2703760/1‑101 (MQ=25)
                                aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                   >  2:754200/1‑101 (MQ=25)
                                 gCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcg                                                                  >  2:1145770/1‑100 (MQ=25)
                                   tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                >  2:7039269/1‑98 (MQ=25)
                                    gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                               >  2:6417559/1‑97 (MQ=25)
                                    gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                               <  1:696610/101‑5 (MQ=25)
                                       ccGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactg                                                            >  1:2911268/1‑94 (MQ=25)
                                        cGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactgg                                                           >  1:4008520/1‑93 (MQ=21)
                                        cGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactgc                                                           >  1:886275/1‑93 (MQ=21)
                                         gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                          >  2:3967130/1‑92 (MQ=21)
                                          tGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggcc                                                         >  1:6656107/1‑91 (MQ=255)
                                                                                  ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                 <  1:5428872/91‑1 (MQ=37)
                                                                                  ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                 >  1:2045659/11‑101 (MQ=37)
                                                                                  ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                 >  2:5410194/11‑101 (MQ=37)
                                                                                   tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                >  2:4803376/10‑101 (MQ=38)
                                                                                   tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                >  2:3218552/10‑101 (MQ=37)
                                                                                   tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                >  2:2571024/10‑101 (MQ=38)
                                                                                   tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                <  1:6133046/92‑1 (MQ=38)
                                                                                    gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc               <  1:3508628/93‑1 (MQ=255)
                                                                                     cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg              <  2:6639009/94‑1 (MQ=255)
                                                                                      gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt             >  2:4347061/7‑101 (MQ=255)
                                                                                       ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg            >  2:6200341/6‑101 (MQ=255)
                                                                                       ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg            <  1:4535580/96‑1 (MQ=255)
                                                                                        cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc           <  2:5134388/97‑1 (MQ=255)
                                                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt          <  1:1089182/98‑1 (MQ=255)
                                                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt          <  2:1931950/98‑1 (MQ=255)
                                                                                         gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt          <  1:3658599/98‑1 (MQ=255)
                                                                                          tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc         <  2:4500534/99‑1 (MQ=255)
                                                                                          tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc         <  1:4394867/99‑1 (MQ=255)
                                                                                          tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc         <  2:3732612/99‑1 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        <  1:1315683/100‑1 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        >  2:6721654/2‑101 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        >  2:22073/2‑101 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        >  2:6054176/2‑101 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        <  1:2760860/100‑1 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        <  2:4042251/100‑1 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        >  1:2954571/2‑101 (MQ=255)
                                                                                           cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa        <  2:421670/100‑1 (MQ=255)
                                                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg       <  2:2348258/101‑1 (MQ=255)
                                                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg       >  1:184781/1‑101 (MQ=255)
                                                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg       >  2:4991937/1‑101 (MQ=255)
                                                                                            gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGGGCTCAg       >  2:2301818/1‑101 (MQ=255)
                                                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc      <  2:3491891/101‑1 (MQ=255)
                                                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc      <  1:3018103/101‑1 (MQ=255)
                                                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc      >  2:6380603/1‑101 (MQ=255)
                                                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc      <  1:4523214/101‑1 (MQ=255)
                                                                                             tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc      <  2:1959520/101‑1 (MQ=255)
                                                                                               aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc    >  1:762203/1‑101 (MQ=255)
                                                                                               aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc    <  2:2516533/101‑1 (MQ=255)
                                                                                                 cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg  >  2:6758671/1‑101 (MQ=255)
                                                                                                 cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg  >  1:2824680/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
GCCGGGTCAGATCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGG  >  NC_014034/681020‑681217

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG