BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,198,718	Δ1 bp	97.7%	coding (151/729 nt)	cobA2 ←	uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,198,718	0	C→.	97.7%	15.4 0.0 ‑0.6	341	coding (151/729 nt)	cobA2	uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand): new base (177/156): ref base (4/4): total (181/160)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.59e-01

Only 100 of 382 total aligned reads displayed.


ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816


 cccGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcc                                                                                                   <  1:4185296/101‑1 (MQ=255)


  ccGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccc                                                                                                  <  2:19417/101‑1 (MQ=255)


    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                >  2:9190831/1‑101 (MQ=255)


    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                <  1:1774083/101‑1 (MQ=255)


    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                <  1:6110136/101‑1 (MQ=255)


    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                >  1:3685130/1‑101 (MQ=255)


    cttGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGTccccc                                                                                                <  2:6236842/100‑1 (MQ=255)


     ttGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccg                                                                                               <  2:1610662/101‑2 (MQ=255)


     ttGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccg                                                                                               <  2:5712364/101‑2 (MQ=255)


     ttGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccg                                                                                               >  2:6178738/1‑100 (MQ=255)


       gCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCtgg                                                                                             >  1:4474355/1‑101 (MQ=255)


        cccGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCgggc                                                                                            <  2:1847924/101‑2 (MQ=255)


        cccGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCgggc                                                                                            >  2:5554513/1‑100 (MQ=255)


          cGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCcgggcgg                                                                                         <  2:3735696/101‑1 (MQ=255)


           ggTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCcgggcggg                                                                                        <  2:8585222/101‑1 (MQ=255)


           ggTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCcgggcggg                                                                                        >  1:8934514/1‑101 (MQ=255)


              cggcgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     <  1:5652047/99‑1 (MQ=255)


              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     >  1:5848672/1‑101 (MQ=255)


              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     <  2:2372553/101‑1 (MQ=255)


              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     >  1:3819375/1‑101 (MQ=255)


              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     <  1:142303/101‑1 (MQ=255)


              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCACCGGGCGGGCAt                                                                                     >  1:1238054/1‑101 (MQ=255)


               aGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATg                                                                                    <  1:2230083/101‑1 (MQ=255)


                gcgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATgg                                                                                   <  1:69177/101‑1 (MQ=255)


                 cgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGc                                                                                  <  2:3544576/101‑1 (MQ=255)


                 cgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGc                                                                                  >  2:3973165/1‑101 (MQ=255)


                 cgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGc                                                                                  >  1:6300373/1‑101 (MQ=255)


                  gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 <  1:4077835/101‑1 (MQ=255)


                  gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 >  2:7282084/1‑101 (MQ=255)


                   cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                <  2:7765934/101‑1 (MQ=255)


                   cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                >  1:5297235/1‑101 (MQ=255)


                    aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGCCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                               >  1:8121050/1‑100 (MQ=255)


                    aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                               >  2:1688719/1‑100 (MQ=255)


                    aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                               <  2:2611587/101‑2 (MQ=255)


                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              <  1:1951715/101‑3 (MQ=255)


                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              >  1:5990219/1‑99 (MQ=255)


                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              >  1:5223424/1‑99 (MQ=255)


                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              >  2:7133762/1‑99 (MQ=255)


                            cgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGc                                                                      >  2:2290463/1‑101 (MQ=255)


                               gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                   <  2:6073295/101‑2 (MQ=255)


                               gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                   <  1:7686212/101‑2 (MQ=255)


                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGTATGG.CCCAGGATCGGcccgg                                                                  >  1:7252393/1‑99 (MQ=255)


                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  <  1:3175086/101‑3 (MQ=255)


                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  1:3241833/1‑99 (MQ=255)


                                 aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                 >  1:751203/1‑98 (MQ=255)


                                 aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                 >  1:4782576/1‑98 (MQ=255)


                                 aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGCCCggg                                                                 >  2:2907492/1‑101 (MQ=255)


                                  aGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggcg                                                                <  2:6975641/101‑5 (MQ=255)


                                  aGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggcg                                                                <  2:24953/101‑5 (MQ=255)


                                    cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                             >  2:6816642/1‑101 (MQ=255)


                                     ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                            <  2:1016029/101‑1 (MQ=255)


                                      gCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaaa                                                           <  1:5474549/101‑1 (MQ=255)


                                         cacaTGATCCTGCTTCGGCTAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAt                                                        <  2:8151979/101‑1 (MQ=255)


                                         cacaTGATCCTGCTTCGGCGAGGGCGGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAt                                                        >  1:7496158/1‑101 (MQ=255)


                                         cacaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAt                                                        >  1:8473581/1‑101 (MQ=255)


                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                       >  1:7897858/1‑101 (MQ=255)


                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  1:2778742/1‑101 (MQ=255)


                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  1:9029678/1‑101 (MQ=255)


                                             tGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtc                                                    <  1:5046077/101‑1 (MQ=255)


                                              gATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtcg                                                   >  1:2562499/1‑101 (MQ=255)


                                              gATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtcg                                                   >  1:8850445/1‑101 (MQ=255)


                                                  cTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACa                                               <  2:6523628/101‑1 (MQ=255)


                                                   tGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAg                                              >  1:332695/1‑101 (MQ=255)


                                                     cTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGca                                            >  1:3540002/1‑101 (MQ=255)


                                                      ttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcac                                           >  2:1124947/1‑101 (MQ=255)


                                                       tCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacc                                          <  1:657522/101‑1 (MQ=255)


                                                       tCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacc                                          <  2:217943/101‑1 (MQ=255)


                                                        cGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacca                                         <  2:6334685/101‑1 (MQ=255)


                                                        cGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacca                                         >  2:7471761/1‑101 (MQ=255)


                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        <  1:4858970/101‑1 (MQ=255)


                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        >  2:5254862/1‑101 (MQ=255)


                                                           cGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCTG.CCCGGCGGAAAGATCGTCGAACAGCACCACAt                                      <  1:6450371/101‑1 (MQ=255)


                                                           cGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACAt                                      >  2:1263585/1‑101 (MQ=255)


                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     <  2:5983249/101‑1 (MQ=255)


                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  2:8415810/1‑101 (MQ=255)


                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  1:951855/1‑101 (MQ=255)


                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     <  1:5163940/101‑1 (MQ=255)


                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     <  1:7056604/101‑1 (MQ=255)


                                                             ggggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                    <  2:1558191/100‑1 (MQ=255)


                                                                 ccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.TCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCt                                <  2:5177319/101‑1 (MQ=255)


                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                              >  1:4370186/1‑101 (MQ=255)


                                                                    cccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTgg                             <  2:5492524/101‑1 (MQ=255)


                                                                     ccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGc                            >  2:450695/1‑101 (MQ=255)


                                                                        gcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAg                         <  1:8627264/101‑1 (MQ=255)


                                                                          ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                       >  2:8800550/1‑101 (MQ=255)


                                                                          ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                       <  1:2038280/101‑1 (MQ=255)


                                                                             cTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGt                    >  2:7998661/1‑101 (MQ=255)


                                                                             cTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGt                    >  1:8011442/1‑101 (MQ=255)


                                                                              ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                   >  2:7724105/1‑101 (MQ=255)


                                                                                gCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCgg                 <  2:8987565/101‑1 (MQ=255)


                                                                                 ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                <  1:1839136/101‑1 (MQ=255)


                                                                                 ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                >  1:8426970/1‑101 (MQ=255)


                                                                                   gACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAg              >  1:6618617/1‑101 (MQ=255)


                                                                                   gACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAg              >  2:1375163/1‑101 (MQ=255)


                                                                                    aCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCCCATCGGCCTTGGCGAGCCGGTCGGCAGc             <  2:5400687/101‑1 (MQ=255)


                                                                                       gcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCttt          <  1:1009791/101‑1 (MQ=255)


                                                                                        cgATCAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  2:4151468/101‑1 (MQ=255)


                                                                                            gAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCg     >  2:3067007/1‑101 (MQ=255)


                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc   <  2:4840856/101‑1 (MQ=255)


                                                                                                tgcgCCCCCGGGGCGGGCATGG.CCCAGGATC.GCCCCGGGGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  <  2:3204365/98‑1 (MQ=255)


ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 28 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG