Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,203 Δ1 bp 1.0% intergenic (‑179/+97) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2030A→. 1.0% 2.6 ‑2.1 ‑0.9418intergenic (‑179/+97)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (194/220):  total (194/224)
Fisher's exact test strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.28e-01

Only 100 of 495 total aligned reads displayed.

GCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAG  >  NC_014034/2640104‑2640303
                                                                                                     |                                                                                                        
gcgttgcAACCGTGGCGGTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGGCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                          <  2:856520/95‑1 (MQ=25)
gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                          <  1:3442538/101‑1 (MQ=25)
 cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCCTGGCC.CTCGGCCCATTCCCCCCCCCAGGGATTTTGAGCCAGAAGaaaa                                                                                                         >  1:5175079/1‑101 (MQ=255)
 cGTGTCAACCGTGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                                         <  1:9103171/101‑2 (MQ=25)
  gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                                        <  1:801475/101‑3 (MQ=25)
  gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                                        <  2:1912966/101‑3 (MQ=25)
  gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                                        <  1:6841081/101‑3 (MQ=25)
     tCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                                                    <  1:712022/101‑1 (MQ=25)
       caCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                  <  2:4783237/100‑1 (MQ=21)
         ccGTGGCGCTTTTGCGCAGGCAAAACGGGGGCGCTGCCCCCCTCTTGGCC.CTCCGCCAATTCACCCCCCCAGGCATTTGGAGCAAGATG.AAAGAGCATg..gg                                                                                              >  2:3852988/1‑101 (MQ=21)
           gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..tt                                                                                          <  2:6832851/101‑1 (MQ=25)
            tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTc                                                                                         <  2:5471634/101‑1 (MQ=25)
             ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCa                                                                                        <  1:8619762/101‑1 (MQ=25)
              gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCAt                                                                                       <  2:789557/101‑1 (MQ=25)
                gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCt                                                                                     <  2:7145618/101‑1 (MQ=21)
                 cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCtt                                                                                    <  1:4678157/101‑1 (MQ=25)
                   tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGc                                                                                  <  2:7390367/101‑1 (MQ=25)
                    ttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGcc                                                                                 <  2:6732890/101‑1 (MQ=25)
                     tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGGATTTGGAGCCAGATG.AAAGACCAAG..GG..TTCATCTTGCCt                                                                                >  2:2577157/1‑101 (MQ=21)
                       cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCCCCCCCCGAGGTCTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCa                                                                              <  1:1595539/101‑1 (MQ=25)
                       cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCa                                                                              <  2:6146053/101‑1 (MQ=21)
                         cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCaaa                                                                            <  2:3178298/101‑1 (MQ=25)
                         cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCaaa                                                                            >  2:1252188/1‑101 (MQ=21)
                          aGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAat                                                                           <  1:6354434/101‑1 (MQ=25)
                           ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAata                                                                          <  1:556535/101‑1 (MQ=25)
                           cccgaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAatat                                                                         >  2:2694194/5‑101 (MQ=17)
                            gCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAatat                                                                         <  1:4395948/101‑1 (MQ=25)
                             cgaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATcc                                                                       >  1:4307606/3‑101 (MQ=17)
                              gaaaAGGGGGTGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATccc                                                                      >  1:5416497/2‑101 (MQ=18)
                              gaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGAC..GG..GG..TTTATCTTGCCCCAAATATccc                                                                      >  1:3313207/2‑101 (MQ=17)
                               aaaAGGGGGCGCTGCCCCCCTCTTGG.CTTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCg                                                                     <  2:524788/101‑1 (MQ=18)
                                aaagggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgg                                                                    >  1:1414557/4‑101 (MQ=17)
                                 aagggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCggg                                                                   >  1:133504/3‑101 (MQ=18)
                                 aGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCgg                                                                    <  2:5403377/101‑1 (MQ=25)
                                  ggggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCggg                                                                   >  1:8418753/1‑101 (MQ=21)
                                  agggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggg                                                                  >  2:7574840/2‑101 (MQ=18)
                                   gggggCGCTG.CCCCCTCTTGGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCC..TTCATCTTGCCGCAAATATCCCgggggg                                                                <  1:7814630/101‑1 (MQ=2)
                                    ggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggg                                                                >  1:2741492/1‑101 (MQ=18)
                                    ggggCGCTG.CCCCCTCTTGGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCC..TTCATCTTGCCGCAAATATCCCGGGGGGa                                                               <  2:3525400/101‑1 (MQ=2)
                                    ggggCGCTG.CCCCCTCTTGGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATG..CCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                               <  2:343243/101‑1 (MQ=2)
                                     gggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtg                                                               <  1:969375/101‑3 (MQ=18)
                                     gggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtg                                                               <  1:5077480/101‑3 (MQ=18)
                                     gggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtg                                                               <  2:8851178/101‑3 (MQ=18)
                                      ggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGa                                                               <  2:4645515/101‑1 (MQ=25)
                                       gcgcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtgag                                                             <  1:3905923/101‑5 (MQ=21)
                                       gcgcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtgag                                                             >  1:215241/1‑97 (MQ=21)
                                         gcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GG..TTTATCTTGCCCCAAATATCCCgggggtgaggc                                                           >  1:8864799/1‑95 (MQ=21)
                                         gcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                            <  2:453995/101‑1 (MQ=25)
                                           tGCGCCCCTC.TGG.C.TTC.GCC.ATTCCCCCCCCGGGATATTTCGGGCAAGATGAAAGGCATCCG..GT..TTCATCTTGCTGCAAATATCCCGGGGGGAGTccgctt                                                       <  1:2520630/101‑5 (MQ=18)
                                           tGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                          <  2:6406878/101‑1 (MQ=25)
                                           tGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                          >  2:1781353/1‑101 (MQ=25)
                                            gCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                         <  1:3640360/101‑1 (MQ=25)
                                              atctctctTGGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                       <  2:651767/97‑1 (MQ=25)
                                                cccTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                     >  1:281226/1‑101 (MQ=37)
                                                 ccTCTTGGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                    <  2:1490003/101‑1 (MQ=37)
                                                 ccTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                    <  2:3066294/101‑1 (MQ=37)
                                                     ttggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                >  1:762615/6‑101 (MQ=37)
                                                       ggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                              >  2:4981206/4‑101 (MQ=37)
                                                          c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                           <  2:5663938/101‑1 (MQ=255)
                                                               ggCCAATTCACCCCCCGNGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                       <  2:2783888/101‑1 (MQ=37)
                                                                 ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                     >  1:487965/1‑101 (MQ=255)
                                                                 ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                     <  1:6536494/101‑1 (MQ=255)
                                                                  cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                    >  1:7936033/1‑101 (MQ=37)
                                                                   aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                   <  2:1111937/101‑1 (MQ=255)
                                                                   aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                   >  1:5758641/1‑101 (MQ=37)
                                                                   aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGAACATG..GG..TTTATCTTGCCTCAAATATCTCGGGGGGAAACAGGAAAGGAGGGGGGGCACGGccca                                   >  2:2931737/1‑100 (MQ=16)
                                                                    aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                  >  2:3266863/1‑101 (MQ=37)
                                                                    aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                  >  1:1093358/1‑101 (MQ=37)
                                                                     ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                 >  2:9118210/1‑101 (MQ=25)
                                                                      tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                >  2:2398873/4‑101 (MQ=25)
                                                                       caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                               >  1:3129534/3‑101 (MQ=255)
                                                                       caccccccGAGGAATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                               >  1:6647924/3‑101 (MQ=25)
                                                                        accccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                              >  1:3041456/2‑101 (MQ=255)
                                                                         ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                             <  2:8331401/101‑1 (MQ=255)
                                                                             ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                         <  2:7947076/101‑1 (MQ=255)
                                                                             ccGACGTAGTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTTCCTCAAATACCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                         <  1:2929819/101‑1 (MQ=37)
                                                                              cGAGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                        >  1:6171331/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                      >  2:5114242/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                      >  1:5816572/1‑101 (MQ=255)
                                                                                 ggTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                     >  2:8158060/1‑101 (MQ=255)
                                                                                 ggTATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                     <  1:8258630/101‑1 (MQ=255)
                                                                                   tATTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCGTtcg                   >  1:7341864/1‑100 (MQ=25)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCNCCTCCCGGGTCTTtctc                  >  2:3675953/1‑101 (MQ=255)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                  <  2:2338201/101‑1 (MQ=255)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                  >  2:4338923/1‑101 (MQ=255)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                  <  2:8754053/101‑1 (MQ=255)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCGTTtctc                  >  1:1948421/1‑101 (MQ=25)
                                                                                     tttGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTTCTCa                 >  2:4029055/1‑101 (MQ=255)
                                                                                      ttGGAGCAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                >  2:7012064/1‑101 (MQ=255)
                                                                                         gagcAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg             >  1:3727543/4‑101 (MQ=255)
                                                                                           gcAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAGATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt           <  2:5899131/100‑1 (MQ=255)
                                                                                           gcAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt           <  2:2080606/100‑1 (MQ=255)
                                                                                            cAAGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc          <  1:1192941/101‑1 (MQ=255)
                                                                                             aaGATG.AAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc         <  1:4584204/101‑1 (MQ=255)
                                                                                               agagtaaaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg        <  2:4242161/96‑1 (MQ=255)
                                                                                                    aaaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa   >  1:523221/1‑101 (MQ=255)
                                                                                                    aaaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa   <  1:4132570/101‑1 (MQ=255)
                                                                                                    aaaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa   <  1:7039886/101‑1 (MQ=255)
                                                                                                     aaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg  >  2:7749470/1‑101 (MQ=255)
                                                                                                     aaGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCACGGGTCTTTCTCAAGCGTTCCGGCTCAg  >  2:6249329/1‑101 (MQ=255)
                                                                                                     |                                                                                                        
GCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAG  >  NC_014034/2640104‑2640303

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG