Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,803,644 Δ1 bp 1.2% coding (913/1263 nt) ttuD ← hydroxypyruvate reductase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,803,6440C→. 1.2% 3.1 ‑3.4 ‑0.0342coding (913/1263 nt)ttuDhydroxypyruvate reductase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (195/143):  total (195/147)
Fisher's exact test strand distribution p-value = 3.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.78e-01

Only 100 of 384 total aligned reads displayed.

CGCAGCGCCGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCACCCGC  >  NC_014034/2803544‑2803744
                                                                                                      |                                                                                                    
cgcAGCGCCGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGgcg.c                                                                                                      <  2:7600575/101‑1 (MQ=255)
     cgcCGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.cccccc                                                                                                 <  1:1837818/101‑1 (MQ=255)
       ccGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.cccccccc                                                                                               <  2:3226744/101‑1 (MQ=255)
        cGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCg                                                                                              >  2:4767135/1‑101 (MQ=255)
        cGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCg                                                                                              >  2:2506337/1‑101 (MQ=255)
        cGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCg                                                                                              <  2:5904762/101‑1 (MQ=255)
         gATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCgg                                                                                             >  2:4072491/1‑101 (MQ=255)
         gATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCgg                                                                                             >  1:4661817/1‑101 (MQ=255)
          aTAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCggg                                                                                            >  2:1791426/1‑101 (MQ=255)
           tAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGc                                                                                           >  1:8745215/1‑101 (MQ=255)
           tAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGc                                                                                           <  2:3963782/101‑1 (MQ=255)
             gCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGa                                                                                         <  1:1578810/101‑1 (MQ=255)
              cAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGAc                                                                                        >  1:7190047/1‑101 (MQ=255)
              cAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGAc                                                                                        <  2:1483322/101‑1 (MQ=255)
              cAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGAc                                                                                        <  2:8325825/101‑1 (MQ=255)
                 aaaTTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAACACCAGCGGCG.CCCGCCCCGGGGGACggg                                                                                     >  2:4295084/1‑101 (MQ=255)
                 aaaTTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCAGCG.CCCCCCCCGGGCGACggg                                                                                     >  1:9158452/1‑101 (MQ=255)
                  aaTTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGCGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGgc                                                                                    >  1:8229819/1‑101 (MQ=255)
                      cAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTg                                                                                >  1:5521550/1‑101 (MQ=255)
                         cATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCa                                                                             >  1:8971288/1‑101 (MQ=255)
                         attccGGCCCGCCCAGA.CGGGGGTTTCCCGCAGGGAAACTTGTCCATCCCCCCCCCGAAAG.AAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCAt                                                                            <  2:3650540/97‑1 (MQ=255)
                          aTTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCAt                                                                            <  2:6132791/101‑1 (MQ=255)
                          aTTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCAt                                                                            <  2:3335082/101‑1 (MQ=255)
                           ttCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATa                                                                           >  1:7524502/1‑101 (MQ=255)
                            tCGGCCCGCCCAGGCCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCCCCCGGGAGCAAAACCAGCGGCGCCCCCCCCCGGGCGACGGGCGCTGGCATa                                                                           <  1:1721933/101‑1 (MQ=255)
                            tCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAg                                                                          <  2:7462035/101‑1 (MQ=255)
                             cGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAgg                                                                         >  1:6457504/1‑101 (MQ=255)
                             cGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAgg                                                                         >  2:8658574/1‑101 (MQ=255)
                             cGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAgg                                                                         >  1:1325121/1‑101 (MQ=255)
                             cGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCCGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAgg                                                                         <  1:4767286/101‑1 (MQ=255)
                                cccgcccAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtcc                                                                      <  1:281767/101‑1 (MQ=255)
                                cccgcccAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtcc                                                                      <  1:350767/101‑1 (MQ=255)
                                 ccgcccAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccg                                                                     <  2:6402242/101‑1 (MQ=255)
                                  cgaccAGACCGAGGTTTACCCGCAGGGAAGC.TGTACATCCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgc                                                                    <  2:6481488/98‑1 (MQ=255)
                                   gcccAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGGCGGGCGCTGGCATAGGgccgcg                                                                   >  1:853668/1‑101 (MQ=255)
                                       aGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccat                                                               >  2:554543/1‑101 (MQ=255)
                                       aGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccat                                                               >  2:5223284/1‑101 (MQ=255)
                                       aGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccat                                                               <  1:7672382/101‑1 (MQ=255)
                                       aGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccat                                                               >  2:4424954/1‑101 (MQ=255)
                                        gACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatc                                                              >  1:2049756/1‑101 (MQ=255)
                                        gACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatc                                                              <  1:6129975/101‑1 (MQ=255)
                                        gACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG..CCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatcc                                                             <  2:8481026/101‑1 (MQ=255)
                                        gACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAACAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatc                                                              <  2:3769725/101‑1 (MQ=255)
                                          ccGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatccg                                                            >  1:7384149/1‑101 (MQ=255)
                                           cGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatccgc                                                           >  2:1889501/1‑101 (MQ=255)
                                           cGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatccgc                                                           >  1:1065681/1‑101 (MQ=255)
                                             gggTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatccgcgc                                                         >  2:5761500/1‑101 (MQ=255)
                                              ggTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGtccgcgccatccgcgcc                                                        <  2:2039872/101‑1 (MQ=255)
                                              ggTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGaccgcgccatccgcgcc                                                        >  2:229479/1‑101 (MQ=255)
                                                 tttCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGt                                                     >  2:1568041/1‑101 (MQ=255)
                                                  ttCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGtt                                                    <  1:4295197/101‑1 (MQ=255)
                                                   tCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGttt                                                   <  2:6828796/101‑1 (MQ=255)
                                                   tCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGttt                                                   <  1:7605564/101‑1 (MQ=255)
                                                    cccGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTc                                                  <  2:1361519/101‑1 (MQ=255)
                                                     ccGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCg                                                 <  2:3560622/101‑1 (MQ=255)
                                                       gCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGcc                                               >  1:7469677/1‑101 (MQ=255)
                                                       gCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGcc                                               >  1:6288948/1‑101 (MQ=255)
                                                       gCAGGGAAAC.TGAACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATGCGCGCCAGTTTCGcc                                               >  2:6691112/1‑101 (MQ=255)
                                                        cAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGccc                                              >  2:5116639/1‑101 (MQ=255)
                                                        cAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGccc                                              >  1:1912457/1‑101 (MQ=255)
                                                         aGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGcccc                                             >  1:228964/1‑101 (MQ=255)
                                                          gggAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGccccc                                            <  1:307294/101‑1 (MQ=255)
                                                           ggAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCa                                           <  1:5818852/101‑1 (MQ=255)
                                                           ggAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCa                                           >  2:5069266/1‑101 (MQ=255)
                                                            gAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAg                                          <  2:484194/101‑1 (MQ=255)
                                                              aaC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGAt                                        >  1:5777190/1‑101 (MQ=255)
                                                              aaC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGAt                                        >  1:7787972/1‑101 (MQ=255)
                                                              aaC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGAt                                        <  1:2506410/101‑1 (MQ=255)
                                                               aC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCAGCGCCAGTTTCCCCCCCAGATc                                       >  1:5181793/1‑101 (MQ=255)
                                                                  tGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCcg                                     >  2:4577187/1‑101 (MQ=255)
                                                                  tGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCcg                                     <  2:8986585/101‑1 (MQ=255)
                                                                   gTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCcgc                                    >  1:5243754/1‑101 (MQ=255)
                                                                    tACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCcgcg                                   >  2:8830230/1‑101 (MQ=255)
                                                                     aCATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCgg                                  >  2:8175137/1‑101 (MQ=255)
                                                                     aCATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCgg                                  <  2:7815762/101‑1 (MQ=255)
                                                                      cATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGc                                 >  1:3938429/1‑101 (MQ=255)
                                                                       aTTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCCCGGCt                                <  1:6032150/101‑1 (MQ=255)
                                                                         tCCGCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTc                              >  1:3754778/1‑101 (MQ=255)
                                                                         tCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTc                              <  2:90478/101‑1 (MQ=255)
                                                                         tCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTc                              >  1:7968175/1‑101 (MQ=255)
                                                                         tCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTc                              >  1:3179109/1‑101 (MQ=255)
                                                                                ccGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTc                       <  1:3317975/101‑1 (MQ=255)
                                                                                 cGAGAGCAAAACTAGCGGCG.CCCCCCCCCGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCa                      >  1:6529348/1‑101 (MQ=255)
                                                                                 cGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCa                      <  2:9120332/101‑1 (MQ=255)
                                                                                       cAAACCCAGCGGCG..CCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATc               <  1:4445617/101‑1 (MQ=255)
                                                                                        aaaaCCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATc               >  2:7216090/1‑101 (MQ=255)
                                                                                        aaaaCCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATc               >  2:53936/1‑101 (MQ=255)
                                                                                         aaaCCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCg              >  1:6895082/1‑101 (MQ=255)
                                                                                           aCCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGcc            <  1:5072378/101‑1 (MQ=255)
                                                                                           aCCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGcc            >  2:286058/1‑101 (MQ=255)
                                                                                             cAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCtt          <  2:8026695/101‑1 (MQ=255)
                                                                                             cAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCtt          <  2:2693404/101‑1 (MQ=255)
                                                                                              aGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTg         <  2:5517611/101‑1 (MQ=255)
                                                                                              aGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTg         <  2:1168253/101‑1 (MQ=255)
                                                                                                cggcg.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCa       <  2:7043713/101‑1 (MQ=255)
                                                                                                 ggcg.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCAc      >  1:5440884/1‑101 (MQ=255)
                                                                                                 ggcg.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCAc      >  1:8401563/1‑101 (MQ=255)
                                                                                                  gcg.cCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCAcc     >  1:559311/1‑101 (MQ=255)
                                                                                                    g.cCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCACCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCACCCg   >  2:6252855/1‑101 (MQ=255)
                                                                                                      ccccccccGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCACCCGc  >  2:7558734/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CGCAGCGCCGATAGCAGAAATTCAACATTCGGCCCGCCCAGACCGGGGTTTTCCCGCAGGGAAAC.TGTACATTCCCCACCCGAGAGCAAAACCAGCGGCG.CCCCCCCCGGGCGACGGGCGCTGGCATAGGTCCGCGCCATCCGCGCCAGTTTCGCCCCCAGATCCGCGGCTTCGCCGGTCAGCGCATCGCCTTGCACCCGC  >  NC_014034/2803544‑2803744

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG