Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,563 +C 2.4% coding (1445/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5631.→C 2.4% 27.5 ‑0.5 ‑0.2534coding (1445/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (249/272):  total (254/280)
Fisher's exact test strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.10e-01

Only 100 of 573 total aligned reads displayed.

AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663
                                                                                                          |                                                                                                     
aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCt                                                                                                        <  2:4598236/101‑1 (MQ=255)
 acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.g                                                                                                      >  1:5670027/1‑101 (MQ=255)
   acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.c                                                                                                   >  1:2656178/1‑101 (MQ=255)
      gTCATCCCCACGCAACCGCCGTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTc                                                                                                <  2:8478439/101‑1 (MQ=255)
      gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTc                                                                                                >  1:7699059/1‑101 (MQ=255)
        catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGc                                                                                              >  2:3951210/1‑101 (MQ=255)
           caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcc                                                                                           >  2:7927571/1‑101 (MQ=255)
            cccacGCAGCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTccc                                                                                          <  1:2982819/100‑1 (MQ=255)
            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTccc                                                                                          >  2:4402883/1‑101 (MQ=255)
            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTccc                                                                                          <  1:7498079/101‑1 (MQ=255)
             ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcccc                                                                                         >  1:2505034/1‑101 (MQ=255)
              cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTccccc                                                                                        <  2:1777056/101‑1 (MQ=255)
               ccGCAACCGCCCTGCACCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcccccc                                                                                       <  2:5065247/100‑1 (MQ=255)
               acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcccccc                                                                                       >  2:5530502/1‑101 (MQ=255)
                cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCcg                                                                                      <  2:3801280/101‑1 (MQ=255)
                 gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCcgc                                                                                     <  1:1612413/101‑1 (MQ=255)
                  cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCcgcg                                                                                    >  1:8381531/1‑101 (MQ=255)
                   aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgg                                                                                   >  1:6357932/1‑101 (MQ=255)
                   aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgg                                                                                   >  2:5424361/1‑101 (MQ=255)
                      cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCggggg                                                                                <  1:9134068/101‑1 (MQ=255)
                       gCCCTGCAGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                               <  2:818028/101‑1 (MQ=255)
                        cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                              >  2:1575672/1‑101 (MQ=255)
                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  2:8056793/101‑1 (MQ=255)
                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                            >  2:1344320/1‑101 (MQ=255)
                             agccgccGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGGCGTAGATCTCGGT.C..TGGGTGCGC.CCG.CGGT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  1:7771251/96‑1 (MQ=2)
                              ccgctgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCC.CCGAACCTGTCGG..CGGC.GAACACCGATGCC.G..CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:8685515/94‑1 (MQ=2)
                               tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:1005609/95‑1 (MQ=0)
                                  ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  1:4871315/101‑1 (MQ=255)
                                   tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  1:5258225/100‑1 (MQ=2)
                                   gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  2:5728400/99‑1 (MQ=2)
                                   gcgTCGCTCCCCCCGCGGGGCCGTGGATCGAAACATCGATGATCAAATC.C..GCAATCGGCCGGG.CTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCTaaa                                                                    <  1:7214851/99‑1 (MQ=2)
                                   cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:2355864/1‑101 (MQ=255)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  1:5829920/100‑1 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                                 >  2:5842104/1‑99 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  2:6447517/1‑101 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  1:3729636/1‑101 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                              <  1:2216633/101‑6 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                              <  1:3645048/101‑6 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               >  2:6927780/1‑100 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               <  1:46087/101‑2 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                >  1:876087/1‑99 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                             <  1:7045905/101‑7 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                               <  1:1195653/101‑4 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                            >  2:511441/1‑97 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                            >  2:2198131/1‑95 (MQ=0)
                                           ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                            <  1:8582630/101‑7 (MQ=0)
                                            cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                           <  2:1915050/101‑8 (MQ=0)
                                            cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                                          <  2:3399870/101‑1 (MQ=255)
                                             gcccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                         <  1:4739566/100‑8 (MQ=0)
                                             ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                         >  1:2759294/1‑94 (MQ=2)
                                              cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                        <  1:5588757/101‑9 (MQ=0)
                                              cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                        <  2:108696/101‑9 (MQ=0)
                                                cgcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                      >  1:6592994/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                    >  2:7052353/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                    >  2:7526678/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACAAC.TCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                   >  1:1335146/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCCGCGCCG.CGCT.GA.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACCTCCGGGGCTCggggg                                                   >  2:4256033/1‑98 (MQ=37)
                                                   gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                   <  2:9040339/101‑1 (MQ=255)
                                                     gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGa                                                 >  2:7403851/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                >  1:1824953/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                <  2:4701243/101‑1 (MQ=255)
                                                         gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                             <  2:6608599/101‑1 (MQ=255)
                                                         gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                             >  2:3538027/1‑101 (MQ=255)
                                                          tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                            >  1:4424096/1‑101 (MQ=255)
                                                           ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                           >  1:4088717/1‑101 (MQ=255)
                                                           ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                           >  1:5388558/1‑101 (MQ=255)
                                                           gcaTCGAAACACC.CCGATCCTGGC.C..CGCCTCAGCGCCT.CGCT.GA.CGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                           <  2:1476252/99‑1 (MQ=38)
                                                            gATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          <  1:7323784/101‑1 (MQ=255)
                                                             aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                         >  2:4893927/1‑101 (MQ=255)
                                                             aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                         <  1:5999456/101‑1 (MQ=255)
                                                               cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                                       >  1:3515207/1‑101 (MQ=255)
                                                               cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                                       >  2:6710074/1‑101 (MQ=255)
                                                               cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGCTCGGCGTGAcgccccccgc                                       >  2:8791386/1‑101 (MQ=38)
                                                                 aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                     >  1:4310151/1‑101 (MQ=255)
                                                                 aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                     >  2:6055311/1‑101 (MQ=255)
                                                                     aac.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                                 <  1:8173532/101‑1 (MQ=255)
                                                                       c.ccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt                               <  1:5071641/101‑1 (MQ=255)
                                                                       c.ccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCggc                               >  1:678023/1‑100 (MQ=255)
                                                                       c.ccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCggc                               >  2:1262011/1‑100 (MQ=255)
                                                                       c.ccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt                               <  1:7942291/101‑1 (MQ=255)
                                                                         ccGACCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                              <  2:7756938/101‑1 (MQ=255)
                                                                           gATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGTCGc                            >  2:5642952/1‑101 (MQ=255)
                                                                              cATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                         <  2:2724631/101‑1 (MQ=255)
                                                                               aTGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        >  1:938789/1‑101 (MQ=255)
                                                                               aTGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        >  1:6237215/1‑101 (MQ=255)
                                                                                 ggC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc                      >  2:7874866/1‑101 (MQ=255)
                                                                                  gC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                     >  1:3320531/1‑101 (MQ=255)
                                                                                   c.c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg                    >  2:5233450/1‑101 (MQ=255)
                                                                                     c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc                   <  2:2355797/101‑1 (MQ=255)
                                                                                     c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc                   >  2:3202341/1‑101 (MQ=255)
                                                                                        cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg                  >  2:3010474/1‑101 (MQ=255)
                                                                                         gCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGGCCCGCCCATCGGTCGCTCCCCCCGCgg                 >  2:6804490/1‑101 (MQ=255)
                                                                                           cccAGCGCCG.CGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg               <  2:7160722/99‑1 (MQ=255)
                                                                                           cTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg               >  2:2030224/1‑101 (MQ=255)
                                                                                           cTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg               >  1:3842105/1‑101 (MQ=255)
                                                                                                   cg.cgCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt       <  2:2607563/101‑1 (MQ=255)
                                                                                                   cg.cgCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt       <  1:1012655/101‑1 (MQ=255)
                                                                                                    g.cgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  1:2035360/101‑1 (MQ=255)
                                                                                                    g.cgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  1:5181601/101‑1 (MQ=255)
                                                                                                        cT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  1:1453694/101‑1 (MQ=255)
                                                                                                          |                                                                                                     
AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG