Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,575,644	+C	100%	intergenic (+46/+127)	corA → / ← RCAP_rcc01455	magnesium/cobalt transport protein CorA/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,575,644	1	.→C	100.0%	212.5	63	intergenic (+46/+127)	corA/RCAP_rcc01455	magnesium/cobalt transport protein CorA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/61):  ref base (0/2):  total (0/63)

TGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGCCATTTG.CGGCG.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGC  >  NC_014034/1575566‑1575723                                                                                |                                                                                 tGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCt                                                            <  2:2439904/101‑1 (MQ=37) tGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCt                                                            <  2:5594305/101‑1 (MQ=37)  gggCCTTCTTCAAATTGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTg                                                           <  2:7420858/101‑1 (MQ=25)   ggCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGc                                                          <  1:3584647/101‑1 (MQ=37)     ccTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCgg                                                        <  2:5182078/101‑1 (MQ=37)       ttcttcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCgggg                                                      <  1:2791117/101‑1 (MQ=37)        tcttcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCggggg                                                     <  1:8263303/101‑1 (MQ=37)          ttcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGAc                                                   <  1:5099864/101‑1 (MQ=37)           tcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACg                                                  <  1:4861641/101‑1 (MQ=37)           tcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACg                                                  <  1:463946/101‑1 (MQ=37)           tcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACg                                                  <  1:274366/101‑1 (MQ=37)           tcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACg                                                  <  2:4067965/101‑1 (MQ=37)            cAAATGGAAGGGCTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGTAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGa                                                 <  1:7971089/101‑1 (MQ=25)            cAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGa                                                 <  2:6913597/101‑1 (MQ=37)             aaaTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGAt                                                <  1:882934/101‑1 (MQ=37)             aaaTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGAt                                                <  2:1772468/101‑1 (MQ=37)             aaaTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGAt                                                <  2:3936505/101‑1 (MQ=37)             aaaTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGAt                                                <  2:7825098/101‑1 (MQ=37)               aTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGt                                              <  1:3123264/101‑1 (MQ=37)               aTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGt                                              <  2:7201136/101‑1 (MQ=37)                  gAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccc                                           <  2:6614526/101‑1 (MQ=37)                  gAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccc                                           <  1:8909682/101‑1 (MQ=37)                  gAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccc                                           <  2:8775354/101‑1 (MQ=37)                  gAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCCGCTCGGGCGCTCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccc                                           <  2:2926174/101‑1 (MQ=25)                   aaGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTcccc                                          <  1:21468/101‑1 (MQ=37)                    aGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccccc                                         <  1:5857887/101‑1 (MQ=37)                    aGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccccc                                         <  1:6081715/101‑1 (MQ=37)                    aGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccccc                                         <  2:1469891/101‑1 (MQ=37)                    aGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccccc                                         <  2:1729562/101‑1 (MQ=37)                    aGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTccccc                                         <  1:6470970/101‑1 (MQ=37)                         tGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGcc                                    <  1:2909328/101‑1 (MQ=37)                            cTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGccccc                                 <  2:5531072/101‑1 (MQ=37)                             tttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCt                                <  1:8893268/101‑1 (MQ=37)                             tttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCt                                <  2:802247/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:5816302/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:4351490/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:4786378/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:1221526/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  1:8422011/101‑1 (MQ=37)                              ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:7771023/101‑1 (MQ=37)                               tAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTg                              <  2:2485030/101‑1 (MQ=37)                               tAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTg                              <  2:7360926/101‑1 (MQ=37)                               tAGGGCCTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGCCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCGTTg                              <  2:8133029/101‑1 (MQ=255)                                  ggCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGt                           <  2:545397/101‑1 (MQ=37)                                  ggCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGt                           <  1:6108045/101‑1 (MQ=37)                                   gCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTa                          <  2:7891996/101‑1 (MQ=37)                                    cTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTAt                         <  2:9195720/101‑1 (MQ=37)                                      tGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTAttt                       <  1:1800916/101‑1 (MQ=37)                                      tGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTAttt                       <  1:3220536/101‑1 (MQ=37)                                       gcgcCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTg                      <  2:8972537/101‑1 (MQ=37)                                       gcgcCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTg                      <  1:6341931/101‑1 (MQ=37)                                       gcgcCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTg                      <  2:5532525/101‑1 (MQ=37)                                           ccTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGcc                  <  2:7528206/101‑1 (MQ=37)                                            cccgcgcgGGTCGGGCGCCCGGAACGGGGGGGGCTGCCGCCC.CCCGCCCCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCa                 <  2:8276074/99‑1 (MQ=255)                                            cTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCa                 <  1:5109574/101‑1 (MQ=37)                                              cgcgcgGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaag               <  2:6658580/101‑1 (MQ=37)                                                 gcgGCTCGGGCCCCCGGAACGCGGGGGCCTGCCGCCC.CCCGCCCCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaagaag            <  1:7823199/101‑1 (MQ=255)                                                  gggCTCGGGCACCCGGAACGCGGGGGTCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAagaaga           <  2:4957505/100‑1 (MQ=255)                                                   ggCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAgaagaa          <  2:1605501/101‑1 (MQ=37)                                                     cTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAAc        <  2:7720197/101‑1 (MQ=37)                                                      gcGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCACCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAAcc       <  1:8548831/100‑1 (MQ=25)                                                         ggCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCt    <  2:4737287/101‑1 (MQ=37)                                                           cgcCCGGAACGCGGGGGGCTG.CCGCCCCCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGc  <  1:7714379/101‑1 (MQ=37)                                                           cgcCCGGAACGCGGGGGGCTG.CCGCCCCCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGc  <  1:6889600/101‑1 (MQ=37)                                                                                |                                                                                 TGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGCCATTTG.CGGCG.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGC  >  NC_014034/1575566‑1575723 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG