BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	90.3%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	90.3%	121.0 ‑1.2 ‑1.2	411	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (165/205): ref base (14/26): total (179/232)
Fisher's exact test strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.62e-02

Only 100 of 436 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


tGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTc                                                                                                    >  2:5126471/1‑101 (MQ=255)


tGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGCGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTc                                                                                                    >  1:2616804/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..g                                                                                                 >  1:143444/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..g                                                                                                 >  2:1205323/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGCGGTGAACTC..g                                                                                                 >  1:5099430/1‑101 (MQ=255)


 gATCCGACGGCAGCACCAGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..g                                                                                                 >  1:7311106/1‑101 (MQ=255)


  aTCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..gg                                                                                                <  2:6697110/101‑2 (MQ=255)


     cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gccc                                                                                           >  1:8398744/1‑100 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCC.Acc                                                                                        >  1:2678868/1‑101 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gcccaac                                                                                        <  2:8590992/101‑5 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gcccaac                                                                                        <  1:334011/101‑5 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gcccaac                                                                                        >  2:3994977/1‑97 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gcccaac                                                                                        <  1:2566639/101‑5 (MQ=255)


          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..gcccaaccc                                                                                      >  2:2100381/1‑95 (MQ=255)


              gaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCgg                                                                                   <  2:1566959/100‑1 (MQ=255)


               aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCggg                                                                                  >  2:3921805/1‑101 (MQ=255)


               aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCggg                                                                                  >  1:4970573/1‑101 (MQ=255)


               aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCggg                                                                                  <  2:8359403/101‑1 (MQ=255)


                ccGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCgggg                                                                                 <  1:2241174/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCt                                                                               <  2:22412/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGc                                                                             <  2:7642659/101‑1 (MQ=255)


                     ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCg                                                                            >  1:4716399/1‑101 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGt                                                                          >  1:3426242/1‑101 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGt                                                                          >  2:361083/1‑101 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTa                                                                         <  2:6075581/101‑1 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTag                                                                        >  1:7034348/1‑100 (MQ=255)


                           tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTagcg                                                                      <  1:7639035/101‑4 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTagcgc                                                                     <  2:849634/101‑5 (MQ=255)


                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTagcgct                                                                    >  2:1031836/1‑96 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                   >  1:7098405/1‑101 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..GA.CCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                   >  1:6307553/1‑101 (MQ=255)


                                   gcgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCg                                                              <  2:7814880/101‑1 (MQ=255)


                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             >  1:425168/1‑101 (MQ=255)


                                    ccgaaggtgagggtggTCGGCGCGGCATCCGTGACCGTGACGATCACCTTGCGGGTGGTAAACTCG.G..CCCAACCCGCGGGGCTGCGGTAGCGCTCGGGCGt                                                             <  1:2919461/96‑1 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  1:3640566/101‑1 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  2:5697988/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:606315/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  2:7107424/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:8025098/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:3194684/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:900964/101‑1 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCCTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       <  1:7207630/101‑1 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       >  1:8984782/1‑101 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       >  1:1149428/1‑101 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  2:6981817/1‑101 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    <  1:2347733/101‑1 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                   <  2:6264956/101‑1 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                   >  1:6415785/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  2:543181/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  1:1811015/101‑1 (MQ=255)


                                                   aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              <  1:2100443/101‑1 (MQ=255)


                                                    tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             <  1:7790426/101‑1 (MQ=255)


                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            >  1:5285371/1‑101 (MQ=255)


                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACTCG.G..CCCAACACTCGGGGCTGCGGTAGCGCTCGGGCGTGTGCGGATCAAGTTCCt                                            >  1:3789277/1‑101 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           >  1:6678623/1‑101 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  2:8119792/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:2885670/101‑1 (MQ=255)


                                                           ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      >  2:1849147/1‑101 (MQ=255)


                                                           ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      <  1:823956/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  2:8841243/1‑101 (MQ=255)


                                                            gCATCCGTGCCCGTTGCGATCACCTTGCGGGTGGTGAACTC..GGCCCC.ACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  2:7342232/101‑1 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  1:4161358/101‑1 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  1:8589146/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   >  1:4616663/1‑101 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:4447688/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:1040020/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:6788485/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  1:1667413/1‑101 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  1:877433/1‑101 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:1149410/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  2:5806535/1‑101 (MQ=255)


                                                                ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                 >  1:7496252/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAggcc                               >  2:2944021/1‑99 (MQ=255)


                                                                   tGACCGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              >  1:2432581/1‑101 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCGTCGt                          >  2:2541645/1‑101 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          >  2:3424868/1‑101 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  2:1978243/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:3712946/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         >  2:421298/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       >  2:8716099/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:5913159/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       >  2:3062157/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:984629/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:6444371/101‑1 (MQ=255)


                                                                           aCGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      <  2:2347270/101‑1 (MQ=255)


                                                                           aCGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      <  2:4970413/101‑1 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  2:3239645/101‑1 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    <  2:5200332/101‑1 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:6591251/1‑101 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  2:2653367/1‑101 (MQ=255)


                                                                                    ttGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc             <  2:1399205/101‑1 (MQ=255)


                                                                                     tGCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTTGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcg            >  2:4542029/1‑101 (MQ=255)


                                                                                      gCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACATGTCCTCGTCATCGGCGAAggcgg           >  1:5595261/1‑101 (MQ=255)


                                                                                      gCGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  2:369227/101‑1 (MQ=255)


                                                                                       cGGGTGGTGAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc          >  1:5823398/1‑101 (MQ=255)


                                                                                          gtggtgAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGgggcggg       >  1:5370610/1‑100 (MQ=255)


                                                                                           tggtgAACTCGGG..CCA.ACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      <  1:7144020/101‑1 (MQ=255)


                                                                                            ggtgAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:4131707/1‑101 (MQ=255)


                                                                                            ggtgAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:3816980/1‑101 (MQ=255)


                                                                                               gAACTCG.G..CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  1:4013844/101‑1 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC..G..CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG