BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,974,330	+G	98.7%	intergenic (‑23/+32)	rpe1 ← / ← cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,974,330	1	.→G	98.7%	5.6 ‑1.0 ‑0.4	392	intergenic (‑23/+32)	rpe1/cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand): new base (200/186): ref base (4/1): total (204/188)
Fisher's exact test strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.71e-01

Only 100 of 418 total aligned reads displayed.


TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GGGC..GG.GGT.G.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAA  >  NC_014034/1974235‑1974421


 ttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGC..gg.ggg                                                                                           >  1:2729682/1‑100 (MQ=255)


    aCGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGC..gg.gggtgt                                                                                        <  1:1970542/101‑5 (MQ=255)


      ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGC..gg.gggggtcc                                                                                      >  1:6349354/1‑95 (MQ=255)


       gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.ccc                                                                                   <  1:2108033/101‑1 (MQ=255)


       gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGC..gg.gggtggcgc                                                                                     >  1:2912542/1‑94 (MQ=255)


        ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.cccc                                                                                  <  2:5217556/101‑1 (MQ=255)


          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.cccccc                                                                                <  1:2548425/101‑2 (MQ=255)


           aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.ccccccg                                                                               <  1:3170549/101‑3 (MQ=255)


           aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.ccccccg                                                                               >  2:843760/1‑99 (MQ=255)


            aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.ccccccgc                                                                              >  2:694902/1‑98 (MQ=255)


              tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.T.ccccccgccc                                                                            <  1:5009754/101‑6 (MQ=255)


               cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCcccgcccg                                                                            >  2:807352/1‑101 (MQ=255)


               cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCcccgcccg                                                                            <  2:8350604/101‑1 (MQ=255)


                  cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAATGTGATGTCGGTCCCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGAt                                                                         <  1:7507307/101‑1 (MQ=255)


                  cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGAt                                                                         >  1:3945363/1‑101 (MQ=255)


                   gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATa                                                                        <  1:3459793/101‑1 (MQ=255)


                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAg                                                                       <  1:818035/101‑1 (MQ=255)


                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAg                                                                       >  2:7621494/1‑101 (MQ=255)


                     aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGc                                                                      <  1:7324257/101‑1 (MQ=255)


                      gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCa                                                                     <  2:7134194/101‑1 (MQ=255)


                      gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCa                                                                     <  2:3998706/101‑1 (MQ=255)


                         ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCAcgg                                                                  >  1:701016/1‑99 (MQ=255)


                         ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCAcgg                                                                  >  2:2627251/1‑99 (MQ=255)


                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCAcggc                                                                 >  2:2088685/1‑98 (MQ=255)


                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCAcggc                                                                 >  1:5881120/1‑98 (MQ=255)


                           cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGC.G.TCCCCCCGCCCGGATAGcgcggcg                                                                >  2:4213763/1‑95 (MQ=255)


                            aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                              >  2:3043215/1‑101 (MQ=255)


                            aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                              >  1:7984349/1‑101 (MQ=255)


                            aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                              >  2:938371/1‑101 (MQ=255)


                             gAAAGGATCGAGGGGGCGATCTTGATGGAACGGGCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGtt                                                             >  1:4776013/1‑101 (MQ=255)


                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                           <  1:1736993/101‑1 (MQ=255)


                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                           >  1:5186438/1‑101 (MQ=255)


                                aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAg                                                          <  1:4568302/101‑1 (MQ=255)


                                 ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGt                                                         >  1:1862712/1‑101 (MQ=255)


                                 ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCCCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGACAGCA.CGGCGTTCCGt                                                         >  2:8948512/1‑101 (MQ=255)


                                 ggATCGAGGGGGCGATCGTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGt                                                         <  2:7100984/101‑1 (MQ=255)


                                   aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                       >  1:8573226/1‑100 (MQ=255)


                                    tCGAGGGGGCGATCTTGATGGAACTGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                      <  2:7326141/101‑3 (MQ=255)


                                    tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                      <  1:5094495/101‑3 (MQ=255)


                                    ctgAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                      <  1:944027/99‑3 (MQ=255)


                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGGGGGGTTGGGGGG..GG.GGG.G.TCCCCCCGCCCGGATAGC.CGGGGGTTCAGttcagc                                                    >  2:8693252/1‑97 (MQ=255)


                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                    >  2:7684243/1‑101 (MQ=255)


                                       aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATCGCA.CGGCGTGCAGTCCACGc                                                   >  2:1496893/1‑101 (MQ=255)


                                       aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                   >  2:3137852/1‑101 (MQ=255)


                                          gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                <  1:4161707/101‑1 (MQ=255)


                                          gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                <  1:1391578/101‑1 (MQ=255)


                                           ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                               >  2:3419576/1‑101 (MQ=255)


                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                              >  2:913538/1‑101 (MQ=255)


                                             cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                             >  2:4870554/1‑101 (MQ=255)


                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                            >  1:7618925/1‑101 (MQ=255)


                                                 cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                         <  2:7930730/101‑1 (MQ=255)


                                                 cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                         >  1:3472012/1‑101 (MQ=255)


                                                 cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                         <  1:6738129/101‑1 (MQ=255)


                                                 cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                         <  2:5612566/101‑1 (MQ=255)


                                                  ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGGGGGGTTG.GGGCG.GG.GGG.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                        >  1:5226584/1‑101 (MQ=255)


                                                  ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                        >  2:5084334/1‑101 (MQ=255)


                                                   tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                       >  1:1528665/1‑101 (MQ=255)


                                                    gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                      <  2:9093456/101‑1 (MQ=255)


                                                    gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcaga                                      >  2:6631499/1‑98 (MQ=255)


                                                      gggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                    <  2:3807306/100‑1 (MQ=255)


                                                       ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                   <  2:6402645/101‑1 (MQ=255)


                                                       ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                   >  2:3486810/1‑101 (MQ=255)


                                                          aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGA.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGACGCGCCAGcc                                >  2:648391/1‑101 (MQ=255)


                                                           cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                               >  1:6116947/1‑101 (MQ=255)


                                                           cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GGGCCGGG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCCGTTCACGCCCAGAGCGACGCGCCAGccc                               <  1:6523021/101‑1 (MQ=255)


                                                            ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                              >  1:8271375/1‑101 (MQ=255)


                                                            ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                              <  2:4625414/101‑1 (MQ=255)


                                                             gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                             >  2:1321017/1‑101 (MQ=255)


                                                             gTCGAAGGTCATGTCGGGCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTTACGCCCAGAGCGACGCGCCAGCCCgg                             <  1:7820577/101‑1 (MQ=255)


                                                              tCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGa                            >  2:1674245/1‑101 (MQ=255)


                                                               ggAAGGTCATGTCGGTTTCCTGAGGGGTTGGGGGG..GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                           <  1:1028674/100‑1 (MQ=255)


                                                                tccgatcttGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                          <  1:4469107/93‑1 (MQ=255)


                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                          >  2:7436359/1‑101 (MQ=255)


                                                                 aaGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                         <  2:1976010/101‑1 (MQ=255)


                                                                   ggTCATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                       <  1:1317581/101‑1 (MQ=255)


                                                                      taTGGCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                    <  1:1130839/100‑1 (MQ=255)


                                                                      cATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGTGG.C.CCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                    <  1:2220424/101‑1 (MQ=255)


                                                                      cATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                    >  1:4930499/1‑101 (MQ=255)


                                                                      cATGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                    >  1:8904490/1‑101 (MQ=255)


                                                                       aTGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                   >  2:2207621/1‑101 (MQ=255)


                                                                       aTGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                   >  1:9142140/1‑101 (MQ=255)


                                                                        tGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaagatcgga                  >  2:4468972/1‑93 (MQ=255)


                                                                        tGTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                  >  1:9117341/1‑101 (MQ=255)


                                                                        tGTAGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                  >  1:7234118/1‑101 (MQ=255)


                                                                         gTCGGTGTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                 <  1:6905783/101‑1 (MQ=255)


                                                                         gTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                 <  2:5797769/101‑1 (MQ=255)


                                                                         gTCGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                 <  2:1750465/101‑1 (MQ=255)


                                                                           cGGTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg               >  1:3004844/1‑101 (MQ=255)


                                                                             gTCTCCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa             >  1:1256800/1‑101 (MQ=255)


                                                                              tctcCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt            <  1:2399668/101‑1 (MQ=255)


                                                                               ctcCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc           >  1:4325843/1‑101 (MQ=255)


                                                                               ctcCTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc           >  1:3693936/1‑101 (MQ=255)


                                                                                 ccTGAGGGGTTG.GGGC..GGAGGA.GCC.CCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGGTAGAGCGTGTCgggcacgt         >  2:2895398/1‑95 (MQ=255)


                                                                                  cTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt        >  1:4257888/1‑101 (MQ=255)


                                                                                  cTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt        <  2:5381964/101‑1 (MQ=255)


                                                                                  cTGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAATTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt        >  1:5411474/1‑101 (MQ=255)


                                                                                   tGAGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGGGCGACGCGCCAGCCCCGATAGAGCTGGCCGGCATCGTTc       >  2:609579/1‑101 (MQ=255)


                                                                                     aGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg     >  2:5881876/1‑101 (MQ=255)


                                                                                     aGGGGTTG.GGGCG.GG.GGT.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg     >  2:1805977/1‑101 (MQ=255)


                                                                                      ggggTTG.GGGCG.GG.GGT.G.TCCCCCCGCACGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg    <  1:3823643/101‑1 (MQ=255)


TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GGGC..GG.GGT.G.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAA  >  NC_014034/1974235‑1974421

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG