BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,047,090	+C	100%	coding (20/879 nt)	potH1 ←	polyamine ABC transporter permease PotH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,047,090	1	.→C	100.0%	145.1	417	coding (20/879 nt)	potH1	polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand): new base (160/210): ref base (28/19): total (188/229)
Fisher's exact test strand distribution p-value = 4.26e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.93e-03

Only 100 of 436 total aligned reads displayed.


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.ACCG.CGGG.TGGAGATGCCGACATACTCTATTC.T.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACcg.c                                                                                                >  2:4247174/1‑101 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACcg.cc                                                                                               >  1:1770245/1‑100 (MQ=255)


 gacgaTGGTCACAAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCAGGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACcg.cc                                                                                               >  1:6566338/1‑100 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgg                                                                                             >  2:2725614/1‑101 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgg                                                                                             >  2:2127704/1‑101 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgg                                                                                             >  2:3524120/1‑101 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggt                                                                                           >  2:3028827/1‑99 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggt                                                                                           >  2:6431746/1‑99 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggt                                                                                           >  1:2543308/1‑99 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGGACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggtt                                                                                          >  2:242158/1‑98 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggtt                                                                                          <  1:7282923/101‑4 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggtt                                                                                          <  2:7396405/101‑4 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggtt                                                                                          >  2:9021281/1‑98 (MQ=255)


       gttCACCGGGGGGGCCGCCAGCAACAGAACTCCATAGATCCGCGTTTGGCTGACCATCACCCAGCCCCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggttg                                                                                         <  1:2929481/99‑5 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggttggag                                                                                      >  1:2448102/1‑94 (MQ=255)


           cccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCG.Ccgggttggaga                                                                                     <  2:6566078/100‑9 (MQ=255)


            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGAt                                                                                     <  1:2736755/101‑1 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATg                                                                                    >  2:591422/1‑101 (MQ=255)


               gCGGGCCCGACACCACCAGAACTGCATGGACCAGCGGTGGGCTGACCAGGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGcc                                                                                  <  2:5089913/101‑1 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGa                                                                                >  2:8635852/1‑101 (MQ=255)


                 gggcCCCCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGa                                                                                <  1:4247290/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGAc                                                                               <  2:8433047/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGAc                                                                               <  1:3209942/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGAc                                                                               <  1:7839098/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGAc                                                                               <  2:4859525/101‑1 (MQ=255)


                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACAt                                                                             <  2:3761757/101‑1 (MQ=255)


                      cgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATAc                                                                           <  2:4626061/101‑1 (MQ=255)


                      cgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATAc                                                                           <  1:609181/101‑1 (MQ=255)


                          aGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTa                                                                       <  2:4471052/101‑1 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                      <  1:4085482/101‑1 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCGACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                     <  1:4778648/101‑1 (MQ=255)


                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                    <  1:3523475/101‑1 (MQ=255)


                                aGAACTGCATAGATCGGCGTTGGGCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.cc                                                               <  2:1868624/101‑1 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.cc                                                               >  2:2845842/1‑101 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.cc                                                               <  2:5617513/101‑1 (MQ=255)


                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccc                                                              >  1:5286102/1‑100 (MQ=255)


                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccc                                                              <  1:4688721/101‑2 (MQ=255)


                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccc                                                              <  1:7113221/101‑2 (MQ=255)


                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccct                                                             <  2:4791644/101‑3 (MQ=255)


                                   acggCATAGTTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctc                                                            <  2:5248694/98‑4 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctc                                                            <  1:378198/101‑4 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctc                                                            <  1:146116/101‑4 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctc                                                            <  2:3959022/101‑4 (MQ=255)


                                   aCTGCATAGACCAGCGTTGGGCTGACCAGGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctc                                                            <  2:8294732/101‑4 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctca                                                           <  2:2543235/101‑5 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctca                                                           <  1:8377683/101‑5 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctca                                                           >  1:2226904/1‑97 (MQ=255)


                                     tGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctcag                                                          <  1:6834433/101‑6 (MQ=255)


                                     tGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATCCACTATTCTT.CCTCAg                                                           >  2:8933550/1‑101 (MQ=255)


                                     tGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctcag                                                          >  2:5479331/1‑96 (MQ=255)


                                     tGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTc.t.ccctcag                                                          <  1:8102261/101‑6 (MQ=255)


                                       cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcg                                                         >  2:5759631/1‑101 (MQ=255)


                                        aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcgc                                                        >  1:6202297/1‑101 (MQ=255)


                                         tAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcgcg                                                       >  1:6707313/1‑100 (MQ=255)


                                           gATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcgcgtc                                                     >  2:6660680/1‑98 (MQ=255)


                                           gATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcgcgtc                                                     >  1:7412858/1‑98 (MQ=255)


                                             tCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAgcgcgtctc                                                   <  2:3243442/101‑6 (MQ=255)


                                              cAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCa                                                  <  1:8503562/101‑1 (MQ=255)


                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCa                                                  >  1:583137/1‑101 (MQ=255)


                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCa                                                  <  1:8615196/101‑1 (MQ=255)


                                                 ggtgggccTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCA.ggc                                              <  2:7650296/94‑3 (MQ=255)


                                                    tGGGCTGACCATGAAGCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:8856212/1‑96 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCA.ggcgcg                                           <  2:2655918/101‑6 (MQ=255)


                                                      ggCTGGCCATGAACCAGCCGCTGGCCGCCCCCGAGCGCGCCC.GCCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGtt                                          <  2:4053306/101‑1 (MQ=255)


                                                       gCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:6323822/101‑1 (MQ=255)


                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGttt                                         >  1:1033921/1‑101 (MQ=255)


                                                        ctaaCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTg                                        <  2:8777363/98‑1 (MQ=255)


                                                        cTGACCAGGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTg                                        <  2:6266044/101‑1 (MQ=255)


                                                         tGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       <  1:3028926/101‑1 (MQ=255)


                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  1:1604253/1‑101 (MQ=255)


                                                          gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      >  1:7994563/1‑101 (MQ=255)


                                                            ccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  1:2022183/101‑1 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  1:4585204/1‑101 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  2:1143589/1‑101 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  1:1139986/1‑101 (MQ=255)


                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                >  2:7793445/1‑101 (MQ=255)


                                                                  cccAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              <  2:1770192/100‑1 (MQ=255)


                                                                      gCCGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcac                          <  2:1399282/101‑1 (MQ=255)


                                                                        cGCTGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        <  2:648322/101‑1 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     <  1:6281530/101‑1 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     <  1:2094972/101‑1 (MQ=255)


                                                                            tgccgccTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  2:1475776/2‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:7585034/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:1354638/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:3401100/1‑101 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  1:9407/101‑1 (MQ=255)


                                                                               cgccTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 <  2:6793729/101‑1 (MQ=255)


                                                                                gccTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                <  1:6957412/101‑1 (MQ=255)


                                                                                 ccTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc               <  1:6431995/101‑1 (MQ=255)


                                                                                  cTCCGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              <  1:5701082/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  2:7087625/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  2:6166661/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  2:2219104/101‑1 (MQ=255)


                                                                                      gAGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          <  2:8040993/101‑1 (MQ=255)


                                                                                       aGCGCGCCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa         >  1:6498334/1‑101 (MQ=255)


                                                                                        gcgcgcCC.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  1:5985430/101‑1 (MQ=255)


                                                                                          gcgccc.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      >  1:6323224/5‑101 (MQ=255)


                                                                                           cgccc.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     <  1:9021703/98‑1 (MQ=255)


                                                                                             ccc.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg   >  2:1172806/2‑101 (MQ=255)


                                                                                              cc.ACCGCCGGGTTGGAGATGCCGACATACTCTATTC.TCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:707187/101‑1 (MQ=255)


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.ACCG.CGGG.TGGAGATGCCGACATACTCTATTC.T.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG