BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,493,952	+G	97.7%	coding (252/252 nt)	RCAP_rcc02319 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,493,952	1	.→G	97.7%	13.6 ‑2.2 ‑1.2	349	coding (252/252 nt)	RCAP_rcc02319	hypothetical protein
Reads supporting (aligned to +/- strand): new base (100/240): ref base (0/8): total (100/249)
Fisher's exact test strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.06e-02

Only 100 of 404 total aligned reads displayed.


TGAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.GGG.GGGG..CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCG  >  NC_014034/2493852‑2494047


tGAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTa.a                                                                                                     <  2:6668151/101‑1 (MQ=255)


tGAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTa.a                                                                                                     <  2:1947291/101‑1 (MQ=255)


tGAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCCGCA.CGACAGGCCG.AGGa.a                                                                                                     >  1:1947192/1‑101 (MQ=255)


     cccGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.ggg.gg                                                                                              >  2:7587533/1‑101 (MQ=255)


     cccGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGGA.A.ggg.gg                                                                                              >  1:572953/1‑101 (MQ=255)


       cGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.ggg.gggg                                                                                            >  1:6857459/1‑101 (MQ=255)


        gAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.ggg.ggggg                                                                                           >  2:6124487/1‑100 (MQ=255)


         aaGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.ggg.gggggc                                                                                          <  2:1740275/101‑3 (MQ=255)


            agCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.ggg.gggggcgct                                                                                       <  1:6617649/101‑6 (MQ=255)


                ccgcGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGccc                                                                                  <  1:5037295/101‑1 (MQ=255)


                   cgcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGcccccc                                                                               <  1:2395011/101‑2 (MQ=255)


                    gcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCGCCGAA.G.GGG.GGGG..CGCTGGCCCCgg                                                                              >  2:7350401/1‑100 (MQ=255)


                    gcgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGGA.AGGGG.GGGG..CGCTGccccccg                                                                              >  1:5878114/1‑99 (MQ=255)


                     cgGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGGA.AGGGG.GGGG..GGCTGccccccgg                                                                             >  2:2693050/1‑98 (MQ=255)


                      ggTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGGTGccccccgtc                                                                            >  1:2060822/1‑97 (MQ=255)


                      ggTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGccccccgtc                                                                            >  2:6270071/1‑97 (MQ=255)


                        tGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCCGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGGA.A.GGGCGGGG..CGCTGccccccggccc                                                                          >  1:1590522/1‑95 (MQ=255)


                        ggATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCg                                                                          <  2:7940441/100‑1 (MQ=39)


                         gATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCCGCCCCCCGTCCGc                                                                         >  2:6454278/1‑101 (MQ=255)


                          aTGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGccccccggccgca                                                                        >  2:8368076/1‑93 (MQ=255)


                            ggCCGAGCGGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAg                                                                      <  2:3126106/101‑1 (MQ=38)


                               cGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..GGCTGGCCCCCGTCCGCAAGCgg                                                                   >  2:1425922/1‑101 (MQ=38)


                               cGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCgg                                                                   >  2:9106749/1‑101 (MQ=38)


                                   cTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTc                                                               <  1:4096641/101‑1 (MQ=37)


                                   cTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTc                                                               <  1:4477869/101‑1 (MQ=37)


                                     gCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTccc                                                             <  2:3430256/101‑1 (MQ=37)


                                     gCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCGGCCCCCCGGCCGCCAGCGGGCCccc                                                             >  2:1848545/1‑101 (MQ=255)


                                      cccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTcccc                                                            <  1:3529719/101‑1 (MQ=37)


                                      cccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGGCCGCAAGCGGACTcccc                                                            >  1:5670693/1‑101 (MQ=37)


                                       ccACCCGCGCGCCGCGTCTGTCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTccccc                                                           <  1:7255227/101‑1 (MQ=25)


                                       ccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCCAGCGGACTccccc                                                           >  2:1154309/1‑101 (MQ=37)


                                       ccACCAGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTccccc                                                           >  1:8838617/1‑101 (MQ=25)


                                          cccCCGCGCGGCGTACGGCCCGCCCCCGCGGCGGGC.CCCGGCACCGACAGGCCT.CGAA.G.GGG.GGGT..CTCTGCCCCCCGTCCCCAAGCGGACTCCCCCCggg                                                       <  1:2211519/101‑1 (MQ=2)


                                            cgcggcccgcTTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGAAAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGa                                                      <  1:2595437/95‑1 (MQ=21)


                                             gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                     <  2:5511536/101‑1 (MQ=25)


                                               gcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGatat                                                   <  2:1010938/101‑1 (MQ=25)


                                                cgccgcGTCTGGCCCAGCGCCGNGGCGGGCNCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAtt                                                  <  1:3358896/101‑1 (MQ=25)


                                                cgccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCCAGCGGACTCCCCCCCGGATAtt                                                  >  2:8497676/1‑101 (MQ=25)


                                                cgccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAtt                                                  <  1:7690192/101‑1 (MQ=25)


                                                 gccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAttt                                                 <  1:8201029/101‑1 (MQ=25)


                                                 gccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAttt                                                 <  2:5002641/101‑1 (MQ=25)


                                                  ccgcGTCTGGCCCAGGGCCGCGGCGGGCGCGCGGCA.GGACGGGCGG.AGTAGG.GGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTg                                                <  1:2289834/101‑1 (MQ=18)


                                                  ccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTg                                                <  1:8124749/101‑1 (MQ=25)


                                                      gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                            <  2:3748850/101‑1 (MQ=25)


                                                       tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..GGCGGCCCCCCCCCCGCAAGAGGAGTCCCCCCCGGGGATTTgtggca                                           >  1:3767209/1‑96 (MQ=255)


                                                       tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                           <  2:8910033/101‑1 (MQ=25)


                                                        cTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                          <  2:7821683/101‑1 (MQ=25)


                                                        cTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                          <  2:565273/101‑1 (MQ=25)


                                                         tGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  2:5389655/101‑1 (MQ=21)


                                                         tGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  1:2648240/101‑1 (MQ=21)


                                                         cggCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                         <  2:8825386/100‑1 (MQ=21)


                                                          ggCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGAAAGa                                        >  1:5377206/1‑101 (MQ=21)


                                                           gCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                       <  2:4721048/101‑1 (MQ=21)


                                                           gCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                       <  2:2901211/101‑1 (MQ=21)


                                                            cccAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                      <  1:4739345/101‑1 (MQ=21)


                                                             ccAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGa                                     <  2:6996257/101‑1 (MQ=21)


                                                             ccAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGa                                     <  2:8083763/101‑1 (MQ=21)


                                                              cAGCGCCGCGGGGGGCGCGCGGCA.CGGCAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                    <  2:4138418/101‑1 (MQ=18)


                                                              cAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                    >  1:4257417/1‑101 (MQ=21)


                                                              cAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                    >  1:9181601/1‑101 (MQ=21)


                                                              cAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                    <  2:5863514/101‑1 (MQ=21)


                                                               cgcgcCGGGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                   <  2:4436789/100‑1 (MQ=18)


                                                               aGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                   >  1:1291021/1‑101 (MQ=21)


                                                                gcgcCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                  <  1:3479673/101‑1 (MQ=21)


                                                                gcgcCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                  >  2:7116961/1‑101 (MQ=21)


                                                                 cgccgcGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                 <  1:6363469/101‑1 (MQ=21)


                                                                 cgccgcGGCGGGCCCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                 <  2:5317057/101‑1 (MQ=18)


                                                                   ccgcGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                               <  1:4852884/101‑1 (MQ=21)


                                                                      cggcggGCGCGCGGCA.CGACAGGCCG.GGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGGAAGATGAAACCGGATg                            >  2:5961040/1‑101 (MQ=18)


                                                                      cggcggGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                            <  2:8683934/101‑1 (MQ=21)


                                                                      cggcggGCGCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                            <  1:311754/101‑1 (MQ=21)


                                                                          gggcgCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                        >  1:5995945/1‑101 (MQ=21)


                                                                           ggcgCGCGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                       <  1:1105478/101‑1 (MQ=21)


                                                                            gcgcgcGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  1:3715740/101‑1 (MQ=21)


                                                                            gcgcgcGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  1:786711/101‑1 (MQ=21)


                                                                            gcgcgcGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  2:2486329/101‑1 (MQ=21)


                                                                            gcgcgcGGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                      <  1:3228848/101‑1 (MQ=21)


                                                                                gcgGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                  >  1:2829069/1‑101 (MQ=21)


                                                                                 cgGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                 <  2:4001678/101‑1 (MQ=21)


                                                                                 cgGCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                 <  2:3788717/101‑1 (MQ=21)


                                                                                  ggCA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                <  1:6153934/101‑1 (MQ=21)


                                                                                  ggCA.CAACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                <  1:6489526/101‑1 (MQ=18)


                                                                                    cA.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc              >  1:6997411/1‑101 (MQ=21)


                                                                                     a.CGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc             <  2:2770154/101‑1 (MQ=21)


                                                                                       cGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg            <  2:2846990/101‑1 (MQ=21)


                                                                                       cGACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg            <  2:3357381/101‑1 (MQ=21)


                                                                                        gACAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa           <  2:1527735/101‑1 (MQ=21)


                                                                                         aCAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa          <  1:8498056/101‑1 (MQ=21)


                                                                                          cAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa         >  2:7452255/1‑101 (MQ=21)


                                                                                          cAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa         >  2:7990327/1‑101 (MQ=21)


                                                                                          cAGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTACCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa         <  2:5231288/101‑1 (MQ=21)


                                                                                           aGGCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAATCTTGCCCGAAat        >  2:3586766/1‑101 (MQ=18)


                                                                                            ggCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata       <  2:6865874/101‑1 (MQ=21)


                                                                                            ggCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata       <  2:4679719/101‑1 (MQ=21)


                                                                                             gCCG.GGTA.A.GGG.GGGGGCCCCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata       <  1:8946637/101‑1 (MQ=18)


                                                                                             gCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat      >  1:864384/1‑101 (MQ=18)


                                                                                             gCCG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat      <  2:2140873/101‑1 (MQ=21)


                                                                                              ccG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc     <  1:52771/101‑1 (MQ=21)


                                                                                               cG.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc    <  1:1848598/101‑1 (MQ=18)


                                                                                                g.AGTA.AGGGG.GGGG..CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc   <  1:7560390/101‑1 (MQ=18)


TGAGCCCCGAAGAGCGCCGCGCGGTGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCA.CGACAGGCCG.AGTA.A.GGG.GGGG..CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCG  >  NC_014034/2493852‑2494047

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG