Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,080 +C 92.0% intergenic (+128/‑114) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0801.→C92.0% 55.4 ‑0.7 ‑0.6261intergenic (+128/‑114)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (156/82):  ref base (12/9):  total (170/91)
Fisher's exact test strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.57e-01

Only 100 of 302 total aligned reads displayed.

GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170
                                                                                                         |                                                                                          
gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc                                                                                          >  2:3447109/1‑101 (MQ=18)
 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                                         >  1:489283/1‑101 (MQ=18)
 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                                         >  2:8319079/1‑101 (MQ=18)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccct                                                                                     <  1:3132562/101‑3 (MQ=17)
      ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccctg                                                                                    <  1:8368439/101‑4 (MQ=17)
        ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCACCGC.CCCCCTGcc                                                                                   >  1:4531221/1‑101 (MQ=17)
         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGccc                                                                                  >  2:4434598/1‑101 (MQ=18)
          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGcccc                                                                                 >  2:2789263/1‑101 (MQ=0)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                 <  2:3818141/101‑1 (MQ=17)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                 <  2:3767100/101‑1 (MQ=17)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                 >  2:1083124/1‑101 (MQ=18)
             atatTTGCGGCAAGATGAAGGGTCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                              <  2:8953323/101‑3 (MQ=2)
              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                                             >  2:7766288/1‑101 (MQ=18)
               atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccgg                                                                            <  1:7555424/101‑1 (MQ=2)
               atTTGCGGCAAGATGAAGGGGCATG.ATTTCACCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccgg                                                                            <  1:6583533/101‑1 (MQ=2)
               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCCC.CCCCCTGCCCcggcgg                                                                            >  2:5454249/1‑101 (MQ=18)
               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                            >  1:4170280/1‑101 (MQ=18)
                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcggcc                                                                          >  1:8007750/1‑99 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.CGCGCCCCCCTGCCCcggcggccg                                                                         >  2:3906303/1‑98 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGCGGGGC.AGCGCCCCCCTGCCCCCGCGGTcc                                                                         >  2:1769121/1‑101 (MQ=17)
                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                        >  2:1361329/1‑101 (MQ=18)
                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                        >  2:664137/1‑101 (MQ=17)
                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                        <  1:700798/101‑1 (MQ=17)
                    cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                                                       <  1:3359253/101‑1 (MQ=17)
                    cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                                                       <  1:1876870/101‑1 (MQ=17)
                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                                    >  1:132532/1‑101 (MQ=18)
                        aataTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  1:2602822/4‑101 (MQ=17)
                        aaGATGAAACCGGATGCCTTTCATGTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  2:4385111/1‑101 (MQ=17)
                        aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TTGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  1:996582/1‑101 (MQ=18)
                         aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  >  2:3636282/1‑101 (MQ=18)
                         aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  >  1:6193330/1‑101 (MQ=17)
                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGACCCCCTGCCCCGGCGGTCCGCGGAGgcgc                                                               >  1:8960017/1‑101 (MQ=17)
                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            >  2:766081/1‑101 (MQ=18)
                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  2:5378781/101‑1 (MQ=17)
                                 ccGGATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          <  1:382543/101‑1 (MQ=17)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  1:5640879/1‑101 (MQ=18)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  2:6788597/101‑1 (MQ=17)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGACCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:4739024/1‑101 (MQ=18)
                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.CGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                        >  1:7676189/1‑101 (MQ=17)
                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                        >  2:5337349/1‑101 (MQ=18)
                                    gATGCCTTTCATCTGACCCGAAATATCCCGGGGGGTGAA.CGGCA.A.AG.CCAG.AGGGGGGCAACGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       <  2:4675163/101‑1 (MQ=18)
                                      tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     <  2:847206/101‑1 (MQ=17)
                                      cgCCTCTAATCTTGCCCGAAATATCCCGGGGGCTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtttgtcgag                                                     <  2:602038/100‑8 (MQ=9)
                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   >  1:6425591/1‑101 (MQ=18)
                                         gttccagctggCCCGAAATAGCCCGGGGGGGGAG.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCGGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  <  1:2805703/91‑1 (MQ=12)
                                          tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  2:4974623/101‑1 (MQ=17)
                                          tttCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGG.GAGCGCCCCCCTGCCCCGGCGGGCCGGGGGGGCGCCTGGGCGGGCgggg                                                 >  1:5824362/1‑101 (MQ=12)
                                             cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  1:7423214/1‑101 (MQ=18)
                                             cATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:8551981/1‑101 (MQ=18)
                                               tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                            >  1:5222058/1‑101 (MQ=18)
                                                cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                                           >  1:5063574/1‑101 (MQ=18)
                                                cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCCGGGGGGCgg                                           >  1:6718963/1‑101 (MQ=17)
                                                     ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  2:7916273/101‑1 (MQ=18)
                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:2693132/101‑1 (MQ=18)
                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:7117265/101‑1 (MQ=18)
                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:1073244/101‑1 (MQ=18)
                                                       gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    >  2:3274496/1‑101 (MQ=18)
                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   >  1:7796682/1‑101 (MQ=18)
                                                         aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaaac                                  >  1:8235956/1‑99 (MQ=18)
                                                         aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                  >  2:1240412/1‑101 (MQ=21)
                                                         aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                  >  2:7392681/1‑101 (MQ=21)
                                                          atatCGCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 >  2:4530900/1‑101 (MQ=21)
                                                          atatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 <  2:4162885/101‑1 (MQ=21)
                                                          atagCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 <  2:489275/101‑1 (MQ=21)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  1:4489356/1‑101 (MQ=18)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  2:9061627/1‑101 (MQ=21)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                <  1:8179405/101‑1 (MQ=21)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  2:2383626/1‑101 (MQ=21)
                                                            atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                               >  2:3126221/1‑101 (MQ=21)
                                                            atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                               >  1:866606/1‑101 (MQ=21)
                                                            atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                               >  2:2021604/1‑101 (MQ=21)
                                                             tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              >  2:1911123/1‑101 (MQ=21)
                                                               ccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  2:5608592/101‑1 (MQ=21)
                                                                    gggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  2:2647800/1‑101 (MQ=25)
                                                                    gggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       <  2:6873914/101‑1 (MQ=25)
                                                                     ggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      >  1:1176138/1‑101 (MQ=25)
                                                                     ggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:2173869/101‑1 (MQ=25)
                                                                     ggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  2:5665382/101‑1 (MQ=25)
                                                                      gTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  2:4095384/1‑101 (MQ=25)
                                                                      gTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATGAATCCAAATTGGAACAg                     >  2:2162884/1‑101 (MQ=19)
                                                                       tGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                    <  1:7463976/101‑1 (MQ=25)
                                                                                   cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  1:2139612/92‑1 (MQ=38)
                                                                                    gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCCTTAACCGAAATTGCAACAGATGCTGCGa            >  2:4484199/9‑101 (MQ=37)
                                                                                    gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:1583342/9‑101 (MQ=38)
                                                                                    gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  1:771172/9‑101 (MQ=38)
                                                                                    cacacggggcgggcgCACGCG..CCCGTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           <  2:6425342/91‑1 (MQ=255)
                                                                                     aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:8239462/8‑101 (MQ=38)
                                                                                     aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:7139071/8‑101 (MQ=38)
                                                                                      aggccagggggggGCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           <  2:3225657/94‑1 (MQ=255)
                                                                                      agccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  1:5572015/7‑101 (MQ=38)
                                                                                      agccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  2:2835201/7‑101 (MQ=38)
                                                                                       gccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  2:3068933/6‑101 (MQ=38)
                                                                                       gccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  1:912172/6‑101 (MQ=38)
                                                                                        ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  2:7535010/5‑101 (MQ=38)
                                                                                          agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTcc      >  1:2343963/3‑100 (MQ=38)
                                                                                          agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTcc      >  2:3958558/3‑100 (MQ=38)
                                                                                          agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:731623/3‑101 (MQ=39)
                                                                                          agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1456791/3‑101 (MQ=39)
                                                                                           gaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  1:1568348/2‑101 (MQ=39)
                                                                                            aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:5041685/1‑101 (MQ=255)
                                                                                                         |                                                                                          
GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG