Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,398 +G 96.2% intergenic (‑108/+22) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3981.→G96.2% 24.7 ‑3.6 ‑0.6288intergenic (‑108/+22)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (124/153):  ref base (9/2):  total (133/155)
Fisher's exact test strand distribution p-value = 2.66e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.79e-01

Only 100 of 295 total aligned reads displayed.

TTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461305‑3461492
                                                                                               |                                                                                              
     aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                                                >  1:8530363/1‑101 (MQ=255)
      cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                                               >  1:485446/1‑101 (MQ=255)
      cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                                               <  1:2566724/101‑1 (MQ=255)
       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                              >  2:5050361/1‑101 (MQ=255)
        cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                             <  1:8661174/101‑1 (MQ=255)
        cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                             >  2:6826571/1‑101 (MQ=255)
         gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                            >  1:5067516/1‑101 (MQ=255)
          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                           <  1:5190763/101‑1 (MQ=255)
          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCACAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCgc                                                                           >  2:4427419/1‑100 (MQ=255)
              ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                       <  2:2434800/101‑1 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  2:422559/1‑101 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  1:2030366/1‑101 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  1:2790291/1‑101 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  1:3190473/1‑101 (MQ=255)
                aGCGGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     >  2:8350432/1‑101 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     >  2:5212379/1‑101 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     >  1:6748939/1‑101 (MQ=255)
                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                                   >  2:5093187/1‑101 (MQ=255)
                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                                   <  2:447614/101‑1 (MQ=255)
                   aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                                  >  1:1168453/1‑101 (MQ=255)
                     gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                                >  2:323304/1‑101 (MQ=255)
                      aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCaaagatctga                                                               <  2:2640310/101‑10 (MQ=255)
                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  1:8377252/101‑1 (MQ=255)
                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  2:1847897/101‑1 (MQ=255)
                        gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                                                             <  1:422567/101‑1 (MQ=255)
                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                            >  2:8192962/1‑101 (MQ=255)
                           cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacgc                                                          >  2:6924795/1‑100 (MQ=255)
                           cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacga                                                          >  1:6602408/1‑101 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                         >  1:2430744/1‑101 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                         >  2:6250372/1‑101 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCCGTCGTTCGATCAGTTCTTGCCGACGAt                                                         <  2:724949/101‑1 (MQ=255)
                             ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                        <  1:3804170/101‑1 (MQ=255)
                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                       <  2:8992142/101‑1 (MQ=255)
                               ggcgGCCAGACGCAGTCTGGCGCCGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCGCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      <  1:4079170/100‑1 (MQ=255)
                               cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      <  1:8061050/101‑1 (MQ=255)
                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                                   >  1:7676149/1‑101 (MQ=255)
                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.TCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 <  1:4285643/101‑1 (MQ=255)
                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 >  2:222345/1‑101 (MQ=255)
                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 >  2:6613460/1‑101 (MQ=255)
                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                                <  2:8773390/101‑1 (MQ=255)
                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                                <  2:9178707/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:9116872/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:8488180/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:4488734/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:603715/101‑1 (MQ=255)
                                       gACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                              <  1:6369964/101‑1 (MQ=255)
                                          gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                           >  1:942823/1‑101 (MQ=255)
                                           cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          <  1:5148012/101‑1 (MQ=255)
                                           cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          <  1:5455739/101‑1 (MQ=255)
                                           cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          >  2:2700468/1‑101 (MQ=255)
                                            aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         <  1:1393903/101‑1 (MQ=255)
                                            aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         >  2:6050824/1‑101 (MQ=255)
                                            aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         <  2:5916736/101‑1 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAAGGGGGCGCAGCCG.CCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTGCTTGCCGACGATGACGTCAGAGGCCTtgat                                       <  1:2717836/101‑1 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       >  1:7570096/1‑101 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       >  2:562902/1‑101 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       <  1:6833656/101‑1 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       >  2:1741593/1‑101 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGCTCTTGCCGACGATGACGCCAGAGGCCTtgat                                       >  1:1030616/1‑101 (MQ=255)
                                                tGGCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:1319561/1‑101 (MQ=255)
                                                tGGCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:1879604/1‑101 (MQ=255)
                                                tGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:6116739/1‑101 (MQ=255)
                                                 ggCGCAGGGCCAAAGGAAGGGGGCGCAGCC.AC....CCGGGCGCG.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    <  1:8193311/101‑1 (MQ=255)
                                                 ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    <  2:8591087/101‑1 (MQ=255)
                                                 ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    <  2:3653646/101‑1 (MQ=255)
                                                  gcgcAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                                   >  2:2834284/1‑101 (MQ=255)
                                                   cgcAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  >  1:3496548/1‑101 (MQ=255)
                                                   cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCAGACGATGACGTCAGAGGCCTTGATGATcg                                  <  1:299108/101‑1 (MQ=255)
                                                    gcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                                 <  2:7654011/101‑1 (MQ=255)
                                                                         gggggcgcagccACCCGGCCGCGCCCCCAACGCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc                  >  2:1648941/11‑101 (MQ=255)
                                                                          ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg                 >  1:6920054/10‑101 (MQ=255)
                                                                            ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc               <  2:3806043/94‑1 (MQ=255)
                                                                            ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc               <  2:4057724/94‑1 (MQ=255)
                                                                            ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc               >  1:4135017/8‑101 (MQ=255)
                                                                             gcgaagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAcc              <  1:7989938/95‑1 (MQ=255)
                                                                              cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt             >  2:2363720/6‑101 (MQ=255)
                                                                              cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCNNNNNGATCGCGCAGGCCTCGTCGCCGACCt             >  1:259618/6‑101 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt            <  2:3446551/97‑1 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt            <  2:7542306/97‑1 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt            <  1:6650587/97‑1 (MQ=255)
                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           <  2:5397229/98‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  2:218658/99‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          >  2:7411393/3‑101 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  2:7076006/99‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  1:7100390/99‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  2:1319529/99‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTCGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          >  1:4673050/3‑101 (MQ=255)
                                                                                  gccgcccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAg         <  1:6892525/94‑1 (MQ=255)
                                                                                  gccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAg         >  2:8296827/2‑101 (MQ=255)
                                                                                  gccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAg         >  2:888085/2‑101 (MQ=255)
                                                                                   ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGCCGATGACGTCAGAGGCCTTGATGATCGAGCAGGCATCGTAGCCGACCTTCAga        >  2:2983022/1‑100 (MQ=255)
                                                                                   ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc        <  1:5673260/101‑1 (MQ=255)
                                                                                     ccccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgcgc      <  1:3415638/97‑1 (MQ=255)
                                                                                       ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGTCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    >  2:5779927/3‑101 (MQ=255)
                                                                                       ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    >  2:5281113/3‑101 (MQ=255)
                                                                                       ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    <  1:1710662/99‑1 (MQ=255)
                                                                                       ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    >  2:4788562/3‑101 (MQ=255)
                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  <  1:754265/101‑1 (MQ=255)
                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  >  1:2271407/1‑101 (MQ=255)
                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  >  2:2785063/1‑101 (MQ=255)
                                                                                               |                                                                                              
TTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461305‑3461492

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG