Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 524,833 +G 1.5% coding (84/1149 nt) RCAP_rcc00492 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034524,8331.→G 1.5% 4.0 0.0 ‑0.2261coding (84/1149 nt)RCAP_rcc00492hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (173/84):  total (176/85)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.14e-01

Only 100 of 434 total aligned reads displayed.

GCGGTAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGACCCCGTCGTC  >  NC_014034/524734‑524933
                                                                                                                                                                                                        
 cGGTAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGATCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCgg                                                                                                    >  1:8562398/1‑101 (MQ=255)
   gTAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCtggg                                                                                                  <  1:8798198/101‑1 (MQ=255)
    tAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCggggg                                                                                                 >  2:5742333/1‑101 (MQ=255)
    tAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCggggg                                                                                                 >  2:7966187/1‑101 (MQ=255)
     aGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCgggggg                                                                                                <  1:5708418/101‑1 (MQ=255)
       atcatcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCgggggggg                                                                                              >  2:3861742/1‑101 (MQ=255)
        tcatcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCggggggggg                                                                                             >  2:3293608/1‑101 (MQ=255)
         catcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGc                                                                                            >  2:2556858/1‑101 (MQ=255)
          cccccGATCGGGGCCAGATGCCCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCt                                                                                           <  1:8955350/99‑1 (MQ=255)
          atcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCt                                                                                           <  1:4780612/101‑1 (MQ=255)
          atcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCt                                                                                           >  1:4192291/1‑101 (MQ=255)
           tcCCGATCGGGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTg                                                                                          <  1:3967194/101‑1 (MQ=255)
           tcCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCTGGGGGGGGCTg                                                                                          <  2:4604162/101‑1 (MQ=255)
            cccGATCGAGGCCAGAGGCCCCTGCGACAGCGAGCCGAGCGGGCCGTCGAGCAGCCCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTgg                                                                                         <  1:7321862/101‑1 (MQ=255)
             ccGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGt                                                                                        >  2:6229087/1‑101 (MQ=255)
                aTCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATc                                                                                     <  1:2069012/101‑1 (MQ=255)
                aTCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGAGAGCCGGCGAGCAGCACCACATGGTCCACGGACCCGGGCGCCGGATGCGGGGGGGGGCTGGTATc                                                                                     >  2:2301390/1‑101 (MQ=255)
                  cGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATcgc                                                                                   >  1:6692231/1‑101 (MQ=255)
                      gTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCgccgcc                                                                               >  1:7034130/1‑101 (MQ=255)
                      gTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCgccgcc                                                                               <  2:807290/101‑1 (MQ=255)
                      gTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCgccgcc                                                                               >  1:2456738/1‑101 (MQ=255)
                       tCAGATGCGCCGGCGCCGGCGGGCCGAGCGTGCCGTCGGGCAGCCCCACATGGTCCACGGACCCGGGCGCCGGACGGGGGGGGGGGCTGGTATCGCCGCCt                                                                              <  1:6994031/101‑1 (MQ=255)
                        cataTGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTg                                                                             <  1:7661907/98‑1 (MQ=255)
                        cAGCTGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCtc                                                                             >  2:1335005/1‑100 (MQ=255)
                        cAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTg                                                                             >  2:445817/1‑101 (MQ=255)
                        cAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTg                                                                             >  2:1210007/1‑101 (MQ=255)
                        cAGATGCGCCGGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTg                                                                             <  1:1773058/101‑1 (MQ=255)
                          gATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGcc                                                                           >  1:1868959/1‑101 (MQ=255)
                          gATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGcc                                                                           <  1:3628216/101‑1 (MQ=255)
                          gATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCGGGGATCGCCGGCTGcc                                                                           >  2:2763228/1‑101 (MQ=255)
                             gcgcCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCa                                                                        >  1:2187140/1‑101 (MQ=255)
                             gcgcCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGGATCGCCGCCTGCCGcc                                                                        >  1:8053335/1‑100 (MQ=255)
                              cgcCGGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCAc                                                                       <  1:3697582/101‑1 (MQ=255)
                              ccccTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCAc                                                                       <  2:3202185/99‑1 (MQ=255)
                               gcCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACt                                                                      >  2:887416/1‑101 (MQ=255)
                               cccTGCGACGGGGAGCCGAGCGTGCCGTCGAGCAGCCCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACt                                                                      <  1:5899282/100‑1 (MQ=255)
                                  tGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGc                                                                   >  1:7151043/1‑101 (MQ=255)
                                    cGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCt                                                                 >  1:3826802/1‑101 (MQ=255)
                                        aGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCg                                                             >  2:5249076/1‑101 (MQ=255)
                                          cgagccgagcGTGCCGTCGAGCAGCACCACAGGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGtt                                                           <  2:5413961/101‑1 (MQ=255)
                                          caacccgagcGCTCGGTCGAGCAGAACCACATGGCCCGCGGGTCCGGGCGCGGGGCGCGGGGGGGGGCTGGTTTCGCCGCCTGCCGCACTGGCCTTGCGtt                                                           <  2:1582795/97‑1 (MQ=255)
                                           gagccgagcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTg                                                          >  2:1860967/1‑101 (MQ=255)
                                           gagccgagcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTg                                                          <  2:8265065/101‑1 (MQ=255)
                                           gagccgagcGTGCCGTCGAGCAGCACCACAGGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTg                                                          <  1:1288500/101‑1 (MQ=255)
                                             gccgagcGTGCCGTCGAGCAGCCCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTgcc                                                        <  1:3541742/101‑1 (MQ=255)
                                              ccgagcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTgccg                                                       >  2:1496850/1‑101 (MQ=255)
                                               cgagcGTGCCGTCGAGCAGCACCACATGGTCCGCGGATCCGCGCGCCGGACGC.GGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTgccgcc                                                     <  2:5014705/101‑1 (MQ=255)
                                                gagcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTgccgcc                                                     >  2:9086789/1‑101 (MQ=255)
                                                gagcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTgccgcc                                                     <  1:796340/101‑1 (MQ=255)
                                                 ggcGTGCCGTCGAGCAGCACCACATGGTCCACGGACCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCa                                                    <  1:4546826/100‑1 (MQ=255)
                                                 agcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCa                                                    >  2:7006878/1‑101 (MQ=255)
                                                  gcGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCAt                                                   <  1:6067986/101‑1 (MQ=255)
                                                    gTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCAttt                                                 >  2:3559827/1‑101 (MQ=255)
                                                    gTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCGGGTATCGCCGCCTGCCGCACTGGCCCTGCGTTGCCGCCAttt                                                 >  1:263425/1‑101 (MQ=255)
                                                      gCCGTCGAGCAGCCCCACATGGCCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTcc                                               <  2:6335669/101‑1 (MQ=255)
                                                       ccGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGGATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTccc                                              >  1:5542815/1‑101 (MQ=255)
                                                        cGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCg                                             >  2:8379627/1‑101 (MQ=255)
                                                          gggaccagctCCGCGTGTTCCACGACTCCGCGCGCGGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGaa                                           <  1:6229326/96‑1 (MQ=255)
                                                           cGAGCAGCCCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAac                                          <  1:5303751/101‑1 (MQ=255)
                                                           cGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAac                                          <  1:1672022/101‑1 (MQ=255)
                                                           cGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAac                                          >  1:6476851/1‑101 (MQ=255)
                                                           cGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAac                                          >  1:5976372/1‑101 (MQ=255)
                                                            gAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAaca                                         <  1:3375757/101‑1 (MQ=255)
                                                             agcagcACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAacac                                        >  1:3076401/1‑101 (MQ=255)
                                                             agcagcACCAAATGGTCCCCGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAacac                                        <  2:3808786/101‑1 (MQ=255)
                                                              gcagcaCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACAcg                                       <  1:4935444/101‑1 (MQ=255)
                                                              gcagcaCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTGGCCGCCATTTCCCGAACAcg                                       >  1:7219548/1‑101 (MQ=255)
                                                               cagccCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACAcgc                                      <  2:6646051/101‑1 (MQ=255)
                                                               cagcaCCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACAcgc                                      >  2:4176633/1‑101 (MQ=255)
                                                                  ccccacATGGTCCACGGACCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGAt                                   <  1:1515448/99‑1 (MQ=255)
                                                                  ccccacATGGCCCCGGAATCCGCGCGCCGGCCGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGAt                                   <  2:48201/99‑1 (MQ=255)
                                                                   cccacAGGGTCCCCGGACCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATg                                  <  2:554827/100‑1 (MQ=255)
                                                                   accacATGGTCCACGGATCCGCGCGCCGGACGC.GGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGc                                 >  1:4903898/1‑101 (MQ=255)
                                                                     cacaTGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCa                                >  2:6814605/1‑101 (MQ=255)
                                                                       cgtGGGCCCCGGACCCGCGCGCGGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAAc                              <  2:7225043/99‑1 (MQ=255)
                                                                       caTGGTCCACGGATCCGCGCGCGGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAAc                              <  1:4509849/101‑1 (MQ=255)
                                                                       caTGGCCCACGGACCCGCGCGCCGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAAc                              <  1:100519/101‑1 (MQ=255)
                                                                        agggtcacgggACCCGCGCGCGGGACGCGGGGGGGGTCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACa                             <  2:7418805/92‑1 (MQ=255)
                                                                        aTGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCCACa                             >  2:7606914/1‑101 (MQ=255)
                                                                          gttCCACGGATCCGCGCCCGGGCCGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGt                           <  2:4280261/99‑1 (MQ=255)
                                                                          ggTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAgc                           >  1:5229908/1‑100 (MQ=255)
                                                                          ggTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGt                           >  1:3193556/1‑101 (MQ=255)
                                                                           ggccccGGACCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTc                          <  2:1074847/96‑1 (MQ=255)
                                                                            gccAGGGTTCCGCGCGCGGGAGGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTcc                         <  2:5955561/100‑1 (MQ=255)
                                                                               aCGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGAc                      >  1:6863220/1‑101 (MQ=255)
                                                                               aCGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGAc                      <  2:3063801/101‑1 (MQ=255)
                                                                                cGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGAcc                     <  1:9136748/101‑1 (MQ=255)
                                                                                cGGACCCGCGCGCCGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGAcc                     <  1:9087211/101‑1 (MQ=255)
                                                                                 ggACCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCa                    <  2:793671/101‑1 (MQ=255)
                                                                                   acccGCGCGCCGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATg                  <  1:1122069/99‑1 (MQ=255)
                                                                                   aTCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATg                  >  2:3733440/1‑101 (MQ=255)
                                                                                     ccGCGCGCCGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGAc                <  1:2790359/101‑1 (MQ=255)
                                                                                      cgcgcgCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGAcg               >  2:2061472/1‑101 (MQ=255)
                                                                                      cgcgcgCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGAcg               <  2:1676372/101‑1 (MQ=255)
                                                                                       gcgcgcGGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGAcgc              <  2:6476601/101‑1 (MQ=255)
                                                                                        cgcgcCGGCCGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGAcgcg             <  1:4608486/101‑1 (MQ=255)
                                                                                          cgcCGGACGGGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGAc           <  1:7786794/101‑1 (MQ=255)
                                                                                           gcgggACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGAcc          <  2:8628322/98‑1 (MQ=255)
                                                                                           gcCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGAcc          >  2:6113279/1‑101 (MQ=255)
                                                                                            ccGGCCGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGAccc         <  1:8267438/101‑1 (MQ=255)
                                                                                                                                                                                                        
GCGGTAGATCATCCCGATCGAGGTCAGATGCGCCTGCGACAGCGAGCCGAGCGTGCCGTCGAGCAGCACCACATGGTCCACGGATCCGCGCGCCGGACGCGGGGGGGGGCTGGTATCGCCGCCTGCCGCACTGGCCTTGCGTTGCCGCCATTTCCCGAACACGCGATGCAACAGTCCGACCATGACGCGACCCCGTCGTC  >  NC_014034/524734‑524933

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG