Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 586,502 +G 98.7% intergenic (‑4/+31) RCAP_rcc00547 ← / ← RCAP_rcc00548 glutaredoxin family protein/BolA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034586,5021.→G98.7% 7.0 ‑0.4 ‑1.2457intergenic (‑4/+31)RCAP_rcc00547/RCAP_rcc00548glutaredoxin family protein/BolA family protein
Reads supporting (aligned to +/- strand):  new base (256/195):  ref base (4/2):  total (260/197)
Fisher's exact test strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.69e-02

Only 100 of 510 total aligned reads displayed.

TGGAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.GGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCTT  >  NC_014034/586402‑586597
                                                                                                      |                                                                                               
 ggAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.g                                                                                                >  1:35393/1‑101 (MQ=255)
  gAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gg                                                                                               >  2:6703951/1‑101 (MQ=255)
  gAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gg                                                                                               >  2:6932182/1‑101 (MQ=255)
  gAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gg                                                                                               <  1:1439682/101‑1 (MQ=255)
  gAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gg                                                                                               >  2:3608076/1‑101 (MQ=255)
   aaaaCCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.ggg                                                                                              >  1:7391393/1‑101 (MQ=255)
   aaaaCCCGCATTGCGGCATTTCCTTCGTGCCCTTCATATAAAGGATGACATCGTTTCCGGCGATATCGGCATTGATCTAGTCGAGCGCAGTCAT.GGGA.ggg                                                                                              >  1:9146371/1‑101 (MQ=255)
    aaaCCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gggg                                                                                             >  1:2419310/1‑100 (MQ=255)
    aaaCCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.gggg                                                                                             >  2:1719216/1‑100 (MQ=255)
        ccGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.ggggtcct                                                                                         <  2:4138000/101‑6 (MQ=255)
        ccGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.ggggtcct                                                                                         >  2:45266/1‑96 (MQ=255)
         cGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCtt                                                                                         <  1:8674881/101‑1 (MQ=255)
         cGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCtt                                                                                         >  2:5989187/1‑101 (MQ=255)
          gCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCCATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCttt                                                                                        >  2:1682841/1‑101 (MQ=255)
           cATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTc                                                                                       >  1:7055890/1‑101 (MQ=255)
            aTTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCt                                                                                      <  1:4425052/101‑1 (MQ=255)
              tGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGc                                                                                    >  1:4619393/1‑101 (MQ=255)
               gCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCg                                                                                   >  1:7811239/1‑101 (MQ=255)
               gCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGAGCGGTCAT.GGGAGGGGTCATTTCTGca                                                                                   >  2:8896109/1‑100 (MQ=255)
                   cATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAAc                                                                               >  2:5119849/1‑101 (MQ=255)
                   cATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAAc                                                                               <  2:508253/101‑1 (MQ=255)
                   cATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAAc                                                                               <  1:2468303/101‑1 (MQ=255)
                    aTTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACg                                                                              <  2:1382251/101‑1 (MQ=255)
                      ttCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTg                                                                            >  2:6532165/1‑101 (MQ=255)
                       tCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGa                                                                           <  1:3963469/101‑1 (MQ=255)
                        ccTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGac                                                                          >  1:6831186/1‑100 (MQ=255)
                        ccTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGaa                                                                          <  2:727763/101‑1 (MQ=255)
                           tCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACcg                                                                       >  2:2682964/1‑101 (MQ=255)
                            cGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACcgc                                                                      >  1:8455646/1‑101 (MQ=255)
                             gTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACcgcg                                                                     >  2:3035477/1‑101 (MQ=255)
                             gTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACcgcg                                                                     <  2:7165349/101‑1 (MQ=255)
                               gCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATGGGCCTTGATCTGGTCGCGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGGGAACCGCGGc                                                                   >  1:2716837/1‑101 (MQ=255)
                               gCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGc                                                                   >  1:6122899/1‑101 (MQ=255)
                               gCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGc                                                                   >  2:7107294/1‑101 (MQ=255)
                                 ccTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTc                                                                 >  1:6773360/1‑101 (MQ=255)
                                  cTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCa                                                                >  2:2811152/1‑101 (MQ=255)
                                  cTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCa                                                                >  1:2932643/1‑101 (MQ=255)
                                   ttCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCAt                                                               <  1:889002/101‑1 (MQ=255)
                                   ttCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCAt                                                               <  1:3383300/101‑1 (MQ=255)
                                    tCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCAtt                                                              >  2:8743053/1‑101 (MQ=255)
                                     cATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTc                                                             >  1:543360/1‑101 (MQ=255)
                                      aTGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCg                                                            >  2:196409/1‑101 (MQ=255)
                                      aTGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCg                                                            <  2:784227/101‑1 (MQ=255)
                                       tGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCgg                                                           >  2:244479/1‑101 (MQ=255)
                                        gTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGc                                                          >  1:3258093/1‑101 (MQ=255)
                                        gTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGc                                                          <  2:7534097/101‑1 (MQ=255)
                                          aaaGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCt                                                        >  1:198005/1‑101 (MQ=255)
                                          aaaGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCt                                                        >  1:6548663/1‑101 (MQ=255)
                                              gATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTtggt                                                    >  2:7724146/1‑101 (MQ=255)
                                               aTGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTtggtg                                                   >  1:5356377/1‑101 (MQ=255)
                                                tGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTtggtgg                                                  <  2:3112939/101‑1 (MQ=255)
                                                 gACATCGTTTCCGGCGATATAGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTggtggt                                                 >  1:8224907/1‑101 (MQ=255)
                                                  aCATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTc                                                <  2:3461359/101‑1 (MQ=255)
                                                   cATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCa                                               <  1:2140482/101‑1 (MQ=255)
                                                   cATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCa                                               <  2:2280899/101‑1 (MQ=255)
                                                    aTCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAg                                              >  1:7928525/1‑101 (MQ=255)
                                                    aTCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAg                                              <  2:1434378/101‑1 (MQ=255)
                                                     tCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAgg                                             >  1:5129031/1‑101 (MQ=255)
                                                       gTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGc                                           >  2:8255609/1‑101 (MQ=255)
                                                       gTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGc                                           >  2:9158677/1‑101 (MQ=255)
                                                       gTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGGCAT.GGGAGGGGTCCTTTCTGCTAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGc                                           >  1:183859/1‑101 (MQ=255)
                                                        tttCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCa                                          >  2:5528969/1‑101 (MQ=255)
                                                               ggATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGt                                   <  2:3214335/100‑1 (MQ=255)
                                                               cGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGt                                   >  1:9195633/1‑101 (MQ=255)
                                                                gATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTg                                  >  1:7175237/1‑101 (MQ=255)
                                                                 atatCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGc                                 >  2:9044010/1‑101 (MQ=255)
                                                                  tatCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCa                                <  1:3608184/101‑1 (MQ=255)
                                                                  tatCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCa                                >  1:371955/1‑101 (MQ=255)
                                                                  tatCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCa                                >  2:4346243/1‑101 (MQ=255)
                                                                   atCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCACGGGCGTGCAg                               >  2:5766688/1‑101 (MQ=255)
                                                                   atCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAg                               <  2:6286648/101‑1 (MQ=255)
                                                                     cGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAgcg                             >  1:429724/1‑101 (MQ=255)
                                                                      ggCCTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAgcgg                            >  1:891646/1‑101 (MQ=255)
                                                                         cTTGATCTGGTCGAGTGCGGTCATGGGGG.GGGCCCTTCCTGCGAACCGTGAACCCCGGCCCTTTGGGCCTTGGGGGCCGGGCCAAGGCCGGCCAgcgggga                         >  1:1224140/1‑98 (MQ=255)
                                                                         cTTGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGgcgc                         >  2:4797499/1‑101 (MQ=255)
                                                                           tGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCAt                       >  1:4812934/1‑101 (MQ=255)
                                                                           tGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCAt                       >  2:4005025/1‑101 (MQ=255)
                                                                           tGATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCAt                       >  1:6071753/1‑101 (MQ=255)
                                                                            gATCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGGGGGCAGCGGCGCAtt                      >  2:7359749/1‑101 (MQ=255)
                                                                             aTCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTc                     >  1:5594299/1‑101 (MQ=255)
                                                                             aTCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTc                     >  2:1302426/1‑101 (MQ=255)
                                                                              tCTGGTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCg                    >  2:4979640/1‑101 (MQ=255)
                                                                                 ggTCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGcc                 >  2:8129451/1‑101 (MQ=255)
                                                                                   tCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCg               <  1:5940312/101‑1 (MQ=255)
                                                                                   tCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCg               <  1:764132/101‑1 (MQ=255)
                                                                                   tCGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCg               >  1:3824960/1‑101 (MQ=255)
                                                                                    cGAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGt              <  1:2811251/101‑1 (MQ=255)
                                                                                     gAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTc             >  1:5783708/1‑101 (MQ=255)
                                                                                     gAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTc             >  1:1716046/1‑101 (MQ=255)
                                                                                     gAGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTc             <  2:8480837/101‑1 (MQ=255)
                                                                                      aGTGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTcc            <  2:6121584/101‑1 (MQ=255)
                                                                                        tGCGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCAt          <  1:6440540/101‑1 (MQ=255)
                                                                                          cGGTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATcg        <  2:4676234/101‑1 (MQ=255)
                                                                                            gTCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATcgcg      >  1:1023529/1‑101 (MQ=255)
                                                                                             tCAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCgcgc     >  1:7695228/1‑101 (MQ=255)
                                                                                              cAT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGcc    <  1:4103570/101‑1 (MQ=255)
                                                                                               aT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCt   >  2:5317609/1‑101 (MQ=255)
                                                                                               aT.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCt   >  2:3649428/1‑101 (MQ=255)
                                                                                                t.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCtt  >  1:1558780/1‑101 (MQ=255)
                                                                                                t.GGGAGGGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCtt  >  2:1059146/1‑101 (MQ=255)
                                                                                                      |                                                                                               
TGGAAAACCCGCATTGCGGCATTTCCTTCGTGCCCTTCATGTAAAGGATGACATCGTTTCCGGCGATATCGGCCTTGATCTGGTCGAGTGCGGTCAT.GGGA.GGGTCCTTTCTGCGAAACGTGAACCGCGGCTCATTCGGCCTTGGTGGTCAGGGCAAGGGCGTGCAGCGGCGCATTCGGCCCGTCCATCGCGCCTT  >  NC_014034/586402‑586597

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG