New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_014034 221956 =561 (1.320)95 (0.220) 13/186 31.7 0.14 coding (701/2748 nt) RCAP_rcc00185 hypothetical protein
?NC_014034 = 222101 580 (1.270)coding (556/2748 nt) RCAP_rcc00185 hypothetical protein
Rejected: Position hash score below cutoff.

TCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCC...........................................  <  NC_014034/222030‑221956
..................................................................................CCGACCGATCCCACGGACCCGACGGACCCGACCGAT  <  NC_014034/222101‑222066
                                                                           |||||||                                    
cccgACGGACCCGACCGATCCCACCGATCCCACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACC                   <  2:4770124/97‑1 (MQ=0)
 gatctGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCC                  <  2:4387566/96‑1 (MQ=0)
 CCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACag                  >  1:8715654/1‑99 (MQ=0)
 CCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  <  1:425958/101‑1 (MQ=0)
 CCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  >  2:866299/1‑101 (MQ=0)
 CCCACGGACCCGACCGCTCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCC                  <  2:7681473/101‑1 (MQ=0)
 CCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  >  2:4062153/1‑101 (MQ=25)
 CCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  >  2:6875543/1‑101 (MQ=25)
 CCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  <  1:3069501/101‑1 (MQ=21)
 CCCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCC                  >  1:5219730/1‑101 (MQ=9)
 CCCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCC                  >  2:6013512/1‑101 (MQ=9)
 CCCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCC                  >  1:299098/1‑101 (MQ=9)
 CCCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCC                  >  1:3156198/1‑101 (MQ=12)
 CCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCCACAGACCC                  >  2:8800581/1‑101 (MQ=0)
 CCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGACCACACGGACCg                  >  2:7248220/1‑100 (MQ=0)
 CCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACAGATCCCACGGACCCGACCGATCCGACGGACCC                  >  1:6102236/1‑101 (MQ=0)
 CCCACCGACCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGCCCGATCTCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCC                  <  1:9114477/101‑1 (MQ=0)
  ccgCGGACCCGACTGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 <  2:8466026/98‑1 (MQ=0)
  CCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 >  1:7859201/1‑101 (MQ=0)
  CCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 >  1:7430658/1‑101 (MQ=0)
  CCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 >  2:3344820/1‑101 (MQ=0)
  CCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 >  1:7709916/1‑101 (MQ=25)
  CCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCa                 >  2:4701938/1‑100 (MQ=12)
  CCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCG                 <  1:1196741/101‑1 (MQ=12)
  CCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCG                 <  1:482453/101‑1 (MQ=12)
  CCACGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCG                 <  1:481174/101‑1 (MQ=12)
  CCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGAGCCGACGGACCCG                 <  1:6600414/101‑1 (MQ=0)
  CCACCGACCCGACCGATCCCACGGATCCCACCGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCG                 <  2:4713120/101‑1 (MQ=0)
   CACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGA                <  1:6432448/101‑1 (MQ=0)
   CACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGA                <  1:2054873/101‑1 (MQ=0)
   CACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGA                <  2:106426/101‑1 (MQ=0)
   CACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCGCGGACCCGACCGACCCCCCGGACCCGA                >  1:8049318/1‑101 (MQ=0)
   CACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGGACCCCACGGACCCGA                >  1:7382549/1‑101 (MQ=12)
    gCGGCCCCGACCGATCCCACGGATCCCACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGAC               <  1:866312/100‑1 (MQ=12)
    cCGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGAC               <  1:3222319/100‑1 (MQ=0)
    cCGGACCCGACCGATCCCCCGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGAC               <  2:7736720/100‑1 (MQ=12)
    ACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGAC               <  1:6709109/101‑1 (MQ=0)
    ACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGAC               >  1:8103153/1‑101 (MQ=0)
    ACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGCCCGATCCCCCGGACCCGAC               >  1:8563657/1‑101 (MQ=0)
    ACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACAGACCAGAC               >  2:4342431/1‑101 (MQ=0)
    ACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCAACGGACCCGACCGATCCCACGGACaccac               >  2:3519367/1‑96 (MQ=9)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  2:4796678/101‑2 (MQ=2)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:7764942/101‑2 (MQ=2)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              >  1:6872790/1‑100 (MQ=0)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              >  1:5281608/1‑100 (MQ=0)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:7615256/101‑2 (MQ=2)
     CGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:7106675/101‑2 (MQ=2)
     CGGACCCGCCCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACc              <  2:3375352/101‑2 (MQ=12)
     CGGACCCGCCCGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  2:5249680/101‑2 (MQ=2)
     CGGACCCGACCGATCCCACGGCCCCGCCCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  2:3308840/101‑2 (MQ=2)
     CGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:6658475/101‑2 (MQ=18)
     CGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:1270657/101‑2 (MQ=21)
     CGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:1535347/101‑2 (MQ=18)
     CGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  1:8270719/101‑2 (MQ=21)
     CGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGGATCCCACGGACCCGACGGATCCAACGGACCCGACG              >  2:5640162/1‑101 (MQ=2)
     CGGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACG              >  1:2367066/1‑101 (MQ=9)
     CGGACCCGACCGATCCCACGGACCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACc              <  2:5921949/101‑2 (MQ=18)
     CGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCCACc              >  1:406272/1‑100 (MQ=0)
     CGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGCCCCGACCGATCCCACGCACCCGACc              >  1:5893933/1‑100 (MQ=2)
     CGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCTGACCGATCCCACGGACCCagac              >  2:8721036/1‑97 (MQ=0)
     CGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACCGACCCGACCCATCCCACGGACCCGACc              >  1:5054732/1‑100 (MQ=2)
     CGGACCCGACCGAGCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACc              <  2:2467573/101‑2 (MQ=0)
      GGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             <  1:2028835/101‑3 (MQ=9)
      GGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             >  2:1023221/1‑99 (MQ=9)
      GGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             <  2:4826485/101‑3 (MQ=9)
      GGAGCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             <  2:7649762/101‑3 (MQ=9)
      GGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             <  2:3592206/101‑3 (MQ=12)
      GGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             >  2:456061/1‑99 (MQ=18)
      GGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGG             >  2:6180373/1‑101 (MQ=25)
      GGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGG             >  2:6059511/1‑101 (MQ=0)
      GGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGG             >  2:9067413/1‑101 (MQ=2)
      GGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGG             >  2:5957791/1‑101 (MQ=2)
      GGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGG             >  1:7473919/1‑101 (MQ=2)
      GGACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGG             >  2:3549206/1‑101 (MQ=2)
      GGACCCGACCGATCCCACGGACCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACcg             <  2:2003611/101‑3 (MQ=12)
      GGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGgccg             >  2:312190/1‑97 (MQ=9)
      GGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGcccg             >  1:1238541/1‑97 (MQ=9)
       GACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGA            >  2:4641449/1‑101 (MQ=2)
        gCCCGACCGATCCCACGGATCCCACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGAC           <  2:826833/100‑1 (MQ=2)
        cCCCGACCGATCCCACGGATCCCACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGAC           <  2:7430334/100‑1 (MQ=2)
        atCCGACCGATCCCACGGATCCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACAGAC           >  2:6657932/3‑101 (MQ=255)
        ACCCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGAC           >  2:1870261/1‑101 (MQ=0)
         CCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACC          <  2:3617283/101‑1 (MQ=14)
          CCGACGGACCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGGACCC         >  1:6226219/1‑101 (MQ=0)
          CCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCC         >  2:1061244/1‑101 (MQ=17)
          CCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCC         <  2:7288369/101‑1 (MQ=17)
          CCGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGACCC         >  1:6956740/1‑101 (MQ=2)
          CCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGACCC         <  1:3358066/101‑1 (MQ=14)
           aGACCGATCCCACGGATCCCACCGATCCGACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGACCCc        <  1:1378340/100‑2 (MQ=255)
           CGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCGACGGACCCGACCGATCCCACGGACCCGACGGACCCc        <  2:3233310/101‑2 (MQ=14)
            cACCGATCCCACGGACCCCACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGGACCCca       <  2:1194454/100‑3 (MQ=0)
            GACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCcc       >  1:5543695/1‑99 (MQ=14)
            GACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACCGATCCCACGGATCCGACGGACCCca       >  2:3887897/1‑99 (MQ=11)
            GACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACCGACCCGACCGACCCCACGGACCCGACGGACCCca       >  1:4024143/1‑99 (MQ=0)
                 ATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACGGACCCCACCGAc  >  2:6598010/1‑100 (MQ=0)
                                                                           |||||||                                    
TCCCACGGACCCGACCGATCCCACGGATCCGACCGATCCCACGGACCCGACCGATCCCACGGACCCGACCGATCC...........................................  <  NC_014034/222030‑221956
..................................................................................CCGACCGATCCCACGGACCCGACGGACCCGACCGAT  <  NC_014034/222101‑222066

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG