Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,5370C→T100.0% 4.3 12intergenic (‑316/‑642)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (5/7):  ref base (0/0):  total (5/7)

CTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434474‑1434579
                                                               |                                            
ctcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:2188054/94‑1 (MQ=0)
ctcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:5752055/94‑1 (MQ=0)
ctcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGAt         <  2:5493554/94‑1 (MQ=0)
  cgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:2135373/6‑101 (MQ=2)
  cgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:5354282/6‑101 (MQ=2)
  cgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:5400059/6‑101 (MQ=2)
  cgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:7469525/6‑101 (MQ=2)
   gatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGGGGATCGa      >  2:1101384/5‑101 (MQ=0)
    atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  1:3398563/98‑1 (MQ=2)
     tcgTCGCTCCCCCCGCGGGGGCGTGGATCTAAACCTATGGGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  2:8826034/99‑1 (MQ=2)
      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac   <  2:5557118/100‑1 (MQ=2)
       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGGGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca  <  1:8740876/101‑2 (MQ=2)
                                                               |                                            
CTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434474‑1434579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG