Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,325,125 G→T 100% P351T (CCC→ACC)  dacC2 ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,325,1250G→T100.0% 1307.0 363P351T (CCC→ACC) dacC2D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  new base (174/187):  ref base (1/1):  total (175/188)

Only 100 of 403 total aligned reads displayed.

CACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCCTTC  >  NC_014034/1325024‑1325225
                                                                                                     |                                                                                                    
   caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                    <  2:5835719/101‑1 (MQ=255)
   caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                    <  2:8100652/101‑1 (MQ=255)
   caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                    <  2:5941398/101‑1 (MQ=255)
   caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGCgc                                                                                                    >  2:3327326/1‑101 (MQ=255)
      cTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCCCCCGCGGCACGGTGCGca                                                                                                 >  2:792172/1‑101 (MQ=255)
      cTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGca                                                                                                 >  1:4804392/1‑101 (MQ=255)
       tGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcag                                                                                                >  2:4265100/1‑101 (MQ=255)
        gACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagc                                                                                               >  1:1394703/1‑101 (MQ=255)
        gACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagc                                                                                               >  2:3825267/1‑101 (MQ=255)
        gACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGcagc                                                                                               >  2:8550559/1‑101 (MQ=255)
            gtgcTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                           <  1:8952944/98‑1 (MQ=255)
            ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                           <  2:2800967/101‑1 (MQ=255)
            ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                           <  2:6675609/101‑1 (MQ=255)
            ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                           <  2:6191097/101‑1 (MQ=255)
            ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                           <  2:8682365/101‑1 (MQ=255)
              tCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagc                                                                                         <  1:8543270/101‑1 (MQ=255)
                tGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagccc                                                                                       >  1:5820855/1‑101 (MQ=255)
                  cgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCg                                                                                     <  1:8246610/101‑1 (MQ=255)
                   gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                    >  1:3493111/1‑101 (MQ=255)
                   gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                    >  1:4516773/1‑101 (MQ=255)
                   gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                    <  2:7403867/101‑1 (MQ=255)
                    ccAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGc                                                                                   >  2:7018238/1‑101 (MQ=255)
                     cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                                  <  2:2888770/101‑1 (MQ=255)
                     cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                                  <  1:7402718/101‑1 (MQ=255)
                      aGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCAc                                                                                 <  1:4102615/101‑1 (MQ=255)
                        aaGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACgg                                                                               >  1:6243010/1‑101 (MQ=255)
                         aGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGt                                                                              <  1:5708364/101‑1 (MQ=255)
                          gCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTg                                                                             <  1:188841/101‑1 (MQ=255)
                            cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                           <  2:3087608/101‑1 (MQ=255)
                            cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                           <  2:322057/101‑1 (MQ=255)
                             ggCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                          <  2:2410860/101‑1 (MQ=255)
                             ggCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                          <  1:4927099/101‑1 (MQ=255)
                             ggCCCCCGGGGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                          <  2:1317153/101‑1 (MQ=255)
                               cccGGGGCGGCGGGGAGGGGGACCAGGAGGCCGCCCACGGGCACGTCGGTGTCCGCCGCCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGGGATTTc                                                                        <  1:2559670/101‑1 (MQ=255)
                                 cgcgGCGGCGGTGAAGCGGCCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                      <  2:1003396/101‑1 (MQ=255)
                                 cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                      <  2:7304423/101‑1 (MQ=255)
                                 cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                      <  2:8813482/101‑1 (MQ=255)
                                  nngnngnnnnTGAAGCGNACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCNAg                                                                     <  2:343843/99‑1 (MQ=255)
                                    ggcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgcc                                                                   <  2:1765621/101‑1 (MQ=255)
                                       ggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAACCCCAGCCCCGGCACGGTGATTTCCAgccgcc                                                                >  2:6749198/1‑101 (MQ=255)
                                        gcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGccc                                                               <  2:2654862/101‑1 (MQ=255)
                                        gcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGccc                                                               >  1:3992755/1‑101 (MQ=255)
                                         cggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGcccc                                                              >  1:2384177/1‑101 (MQ=255)
                                         cggGGAAGCGGACCAGGAGGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGcccc                                                              <  2:8527007/101‑1 (MQ=255)
                                          ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                             <  2:7582578/101‑1 (MQ=255)
                                          ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                             >  1:7951103/1‑101 (MQ=255)
                                          ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                             <  1:433262/101‑1 (MQ=255)
                                           gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                            <  1:5466059/101‑1 (MQ=255)
                                           gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                            <  2:5037467/101‑1 (MQ=255)
                                             gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                          <  2:2114355/101‑1 (MQ=255)
                                               aGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCg                                                        <  1:7528662/101‑1 (MQ=255)
                                                 cGGACCAGGAAGCCGCCCTCGGCCCCGTCGGTTTCCGCCACCACTGTCACGGTGCGCAGCTCCAGCCCCGCCACCGAGATTTCCACCCGCCCCAGCTCGGt                                                      >  2:4499659/1‑101 (MQ=255)
                                                 cGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGt                                                      <  1:3305140/101‑1 (MQ=255)
                                                  ggACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTg                                                     <  1:8501527/101‑1 (MQ=255)
                                                  ggACCAGGAAGCCGCCCTCGGCCACGCCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTg                                                     >  1:5274463/1‑101 (MQ=255)
                                                   gACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGc                                                    <  2:9063775/101‑1 (MQ=255)
                                                   gACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGc                                                    <  2:3888603/101‑1 (MQ=255)
                                                    aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                                   >  2:2552938/1‑101 (MQ=255)
                                                    aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                                   <  1:8725854/101‑1 (MQ=255)
                                                     cccggAACCCGCCCCCGGCCACGTCGGTTTCCGCCCCCAGCCGCACGGTGCGCAGCCCCAGCCCCGGCATGGTGATTTCCAGCCGCCCCAGCTCGGTGccc                                                  <  1:6403866/98‑1 (MQ=255)
                                                       aGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgc                                                >  2:6327051/1‑101 (MQ=255)
                                                       aGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgc                                                >  1:6135556/1‑101 (MQ=255)
                                                                cccTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCgg                                       >  2:2696417/1‑101 (MQ=255)
                                                                 ccTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGgc                                      <  1:8060200/101‑1 (MQ=255)
                                                                 ccTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGgc                                      >  2:3595419/1‑101 (MQ=255)
                                                                     ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                                  >  2:1059220/1‑101 (MQ=255)
                                                                       ccACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTg                                <  1:6859209/101‑1 (MQ=255)
                                                                          cGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAcc                             <  1:6004043/101‑1 (MQ=255)
                                                                          cGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAcc                             >  1:7171114/1‑101 (MQ=255)
                                                                          cGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAcc                             >  2:7874733/1‑101 (MQ=255)
                                                                             cGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCggg                          >  1:7377001/1‑101 (MQ=255)
                                                                             cGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCggg                          >  2:4817856/1‑101 (MQ=255)
                                                                             cGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCggg                          >  2:5357135/1‑101 (MQ=255)
                                                                               gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                        >  1:3641475/1‑101 (MQ=255)
                                                                               gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGCGcc                        >  1:1695699/1‑101 (MQ=255)
                                                                                 ttCCGCCACCAGCGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGt                      <  2:3943107/101‑1 (MQ=255)
                                                                                  tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                     <  1:1116065/101‑1 (MQ=255)
                                                                                  tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                     <  1:2871891/101‑1 (MQ=255)
                                                                                   ccGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGTCCGTCa                    <  2:6119716/101‑1 (MQ=255)
                                                                                   ccGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCa                    <  1:8434046/101‑1 (MQ=255)
                                                                                    cGCCCCCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                   <  2:5586691/101‑1 (MQ=255)
                                                                                    cGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                   >  1:1582419/1‑101 (MQ=255)
                                                                                    cGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                   >  2:8258204/1‑101 (MQ=255)
                                                                                     cccaccaGCGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGCGACCGGGCCGTCaaa                  <  1:8841842/100‑1 (MQ=255)
                                                                                     cccaccaGCGCCACGGTGCCCAGCCCCTGCCCCGGATCGGTCATTTCCCGCCGCCCCAGCGCGGTCCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaaa                  <  2:3998014/100‑1 (MQ=255)
                                                                                      cccccaGCGGCCCGGTGCGCACCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg                 <  2:8946037/98‑1 (MQ=255)
                                                                                      ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg                 >  2:313428/1‑101 (MQ=255)
                                                                                      ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg                 <  2:1447162/101‑1 (MQ=255)
                                                                                       caccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAgc                >  2:6450277/1‑100 (MQ=255)
                                                                                       caccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGa                <  1:2025307/101‑1 (MQ=255)
                                                                                       caccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGa                <  2:3678299/101‑1 (MQ=255)
                                                                                        cccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAc               <  1:1763712/100‑1 (MQ=255)
                                                                                        accaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAc               >  2:1626977/1‑101 (MQ=255)
                                                                                        accaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAc               >  2:1896715/1‑101 (MQ=255)
                                                                                         ccaGCGGCACGGTGCCCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc              <  2:4891593/101‑1 (MQ=255)
                                                                                           aGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGc            >  1:3040527/1‑101 (MQ=255)
                                                                                           aGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGc            >  2:1523439/1‑101 (MQ=255)
                                                                                             gggCACGGTGCCCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt          <  1:3825376/100‑1 (MQ=255)
                                                                                              ggCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTc         <  2:8331690/101‑1 (MQ=255)
                                                                                                  cGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGcc     >  2:7143704/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCCTTC  >  NC_014034/1325024‑1325225

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG