Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,342,930 A→G 0.8% E422E (GAA→GAG RCAP_rcc01264 → reverse transcriptase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,342,9300A→G 0.8% 2.2 ‑3.8 ‑0.1620E422E (GAA→GAGRCAP_rcc01264reverse transcriptase
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (326/288):  total (326/294)
Fisher's exact test strand distribution p-value = 2.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.85e-01

Only 100 of 648 total aligned reads displayed.

CCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAGGG  >  NC_014034/1342830‑1343030
                                                                                                    |                                                                                                    
ccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                      <  1:4180267/101‑1 (MQ=255)
   aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                                   <  2:6507159/101‑1 (MQ=255)
    ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGAGGGCAGACGAAATaa                                                                                                  >  1:81889/1‑101 (MQ=255)
    ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                                  <  1:6703603/101‑1 (MQ=255)
     tttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaaa                                                                                                 <  1:1907979/101‑1 (MQ=255)
     tttGACAAATATGGGCTTGAGGATAAGAG..GAGATATTTGTCTGCTGACCCCCCACTAATTTTCTGCTCGTGTTGAAAGCATGACGGCAGATGAAATAAACg                                                                                               <  2:3584512/101‑1 (MQ=255)
        gACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGc                                                                                              >  1:4444393/1‑101 (MQ=255)
           aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                           >  2:6324174/1‑101 (MQ=255)
           aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                           >  1:4967914/1‑101 (MQ=255)
           aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGCGTTGAAAGCATGACGGCAGACGaaagatcggaaga                                                                                           >  2:6616776/1‑91 (MQ=255)
            aaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCa                                                                                          >  1:2383379/1‑101 (MQ=255)
            aaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCa                                                                                          >  2:4617890/1‑101 (MQ=255)
            aaTATGGGCTTCAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCa                                                                                          >  1:6208323/1‑101 (MQ=255)
               atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                       >  2:887660/1‑101 (MQ=255)
                tGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTcc                                                                                      <  1:6766743/101‑1 (MQ=255)
                 gggCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCg                                                                                     >  1:2259704/1‑101 (MQ=255)
                  ggCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGa                                                                                    <  1:7026900/101‑1 (MQ=255)
                   gCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGaa                                                                                   >  1:2812873/1‑101 (MQ=255)
                   gCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGaa                                                                                   >  1:2964089/1‑101 (MQ=255)
                    cTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAAc                                                                                  >  1:8251235/1‑101 (MQ=255)
                     ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                                 >  2:7827585/1‑101 (MQ=255)
                     ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                                 >  1:4545565/1‑101 (MQ=255)
                      tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                                >  1:5391940/1‑101 (MQ=255)
                      tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                                >  1:936054/1‑101 (MQ=255)
                      tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                                >  2:1530350/1‑101 (MQ=255)
                       gAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTg                                                                               >  1:579249/1‑101 (MQ=255)
                        aGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGc                                                                              <  2:8228803/101‑1 (MQ=255)
                          gATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAt                                                                            <  1:8795142/101‑1 (MQ=255)
                           aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                           <  1:3839006/101‑1 (MQ=255)
                           aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                           <  2:18316/101‑1 (MQ=255)
                           aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                           <  2:5745852/101‑1 (MQ=255)
                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          <  1:8485147/101‑1 (MQ=255)
                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          <  2:2963991/101‑1 (MQ=255)
                            tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                          >  2:909834/1‑101 (MQ=255)
                             aaGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTaa                                                                         <  2:2332233/101‑1 (MQ=255)
                             aaGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTaa                                                                         >  1:6831325/1‑101 (MQ=255)
                             aaGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTaa                                                                         >  1:2091647/1‑101 (MQ=255)
                              agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                        <  2:2835017/101‑1 (MQ=255)
                               gagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGAATGAAGGCAGACGAAATAAACGATGCATTCCGAACTTGCATTaaaa                                                                       >  1:4275136/1‑99 (MQ=255)
                                agGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACaa                                                                      <  2:1954019/101‑1 (MQ=255)
                                agGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACaa                                                                      >  1:7649344/1‑101 (MQ=255)
                                 ggCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAg                                                                     >  2:1244047/1‑101 (MQ=255)
                                   cGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGa                                                                   >  1:1530545/1‑101 (MQ=255)
                                    gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaa                                                                  >  2:3783082/1‑101 (MQ=255)
                                    gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaa                                                                  >  1:1116223/1‑101 (MQ=255)
                                     agaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaaa                                                                 <  2:7404483/101‑1 (MQ=255)
                                     agaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaaa                                                                 >  2:3959058/1‑101 (MQ=255)
                                      gaTATTTGTCTGCTGGCCCCGCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGCAAt                                                                <  1:7201542/101‑1 (MQ=255)
                                      gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                                <  1:4755616/101‑1 (MQ=255)
                                      gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                                <  1:1424767/101‑1 (MQ=255)
                                        tatTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtct                                                              <  2:5124837/101‑1 (MQ=255)
                                           ttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCgg                                                           <  1:6424986/101‑1 (MQ=255)
                                            tGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGa                                                          <  2:69093/101‑1 (MQ=255)
                                            tGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGa                                                          >  2:5476905/1‑101 (MQ=255)
                                              tCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGata                                                        <  1:5585654/101‑1 (MQ=255)
                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAACCGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       <  2:4425360/101‑1 (MQ=255)
                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       >  2:4047090/1‑101 (MQ=255)
                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       >  2:258722/1‑101 (MQ=255)
                                               ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                       >  2:1699959/1‑101 (MQ=255)
                                                    ggCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgaga                                                  <  1:2701260/101‑1 (MQ=255)
                                                    ggCCCCCCACTTATTTTCTGCTCGTGTCAAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgaga                                                  <  1:5070827/101‑1 (MQ=255)
                                                      ccccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGaaa                                                <  2:4846887/101‑1 (MQ=255)
                                                       cccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAg                                               >  1:2480502/1‑101 (MQ=255)
                                                       cccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAg                                               >  1:6021855/1‑101 (MQ=255)
                                                           cACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCt                                           >  2:2123741/1‑101 (MQ=255)
                                                           cACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCt                                           >  1:2629622/1‑101 (MQ=255)
                                                           cACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCt                                           <  1:7996920/101‑1 (MQ=255)
                                                            aCTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTg                                          <  2:6021620/101‑1 (MQ=255)
                                                            aCTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTg                                          <  1:1010087/101‑1 (MQ=255)
                                                               tATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCtt                                       >  1:3412594/1‑101 (MQ=255)
                                                                aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTg                                      <  1:5211496/101‑1 (MQ=255)
                                                                aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTg                                      >  2:4990349/1‑101 (MQ=255)
                                                                  tttCTGCTCGTGTTGAAAGCATGACGGCAGACTAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAg                                    >  1:3738571/1‑101 (MQ=255)
                                                                  tttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAg                                    >  2:8655986/1‑101 (MQ=255)
                                                                  tttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAg                                    >  1:3138344/1‑101 (MQ=255)
                                                                     cTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCa                                 >  2:6736969/1‑101 (MQ=255)
                                                                     cTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCa                                 >  2:6173296/1‑101 (MQ=255)
                                                                       gCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGATGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATAGAGAAAGACCTGCTTGAGGCAtt                               >  1:9087766/1‑101 (MQ=255)
                                                                          cGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTc                            <  1:1991909/101‑1 (MQ=255)
                                                                          cGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTc                            >  1:7837002/1‑101 (MQ=255)
                                                                            tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                          >  2:7609743/1‑101 (MQ=255)
                                                                            tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAGCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                          >  1:6573528/1‑101 (MQ=255)
                                                                             gtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTa                         >  2:1054422/1‑101 (MQ=255)
                                                                               tGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAAt                       <  2:4322247/101‑1 (MQ=255)
                                                                                gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                      >  1:3551040/1‑101 (MQ=255)
                                                                                 aaaGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGt                     <  2:3274572/101‑1 (MQ=255)
                                                                                   aGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTc                   <  2:6821500/101‑1 (MQ=255)
                                                                                     cATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGc                 >  2:5226981/1‑101 (MQ=255)
                                                                                      aTGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCTTTTTCGTAATGTTCGcc                <  1:8924905/101‑1 (MQ=255)
                                                                                      aTGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGcc                >  1:646856/1‑101 (MQ=255)
                                                                                       tGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCa               <  1:3319912/101‑1 (MQ=255)
                                                                                        gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg              >  2:7123976/1‑101 (MQ=255)
                                                                                        gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg              >  2:272701/1‑101 (MQ=255)
                                                                                        gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg              <  1:1702932/101‑1 (MQ=255)
                                                                                            gCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATg          >  1:7542515/1‑101 (MQ=255)
                                                                                             cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa         >  2:8370940/1‑101 (MQ=255)
                                                                                             cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa         >  2:8551119/1‑101 (MQ=255)
                                                                                              aGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGAc        >  1:6321812/1‑101 (MQ=255)
                                                                                               gACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACg       <  2:3412490/101‑1 (MQ=255)
                                                                                                 cGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTa     >  1:4366478/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACGTAGGG  >  NC_014034/1342830‑1343030

Base quality scores:  ATCG  < 3 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG