Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,565 T→C 20.4% intergenic (+377/‑554) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5650T→C20.4% 205.7 ‑0.6 ‑0.2600intergenic (+377/‑554)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (42/41):  ref base (185/209):  total (288/312)
Fisher's exact test strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.94e-01

Only 100 of 643 total aligned reads displayed.

CATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1395465‑1395665
                                                                                                        |                                                                                                    
 aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                                                     >  1:8521109/1‑101 (MQ=255)
    gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgtc                                                                                                  >  2:9048046/1‑101 (MQ=255)
         tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TGGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                                                             >  1:6572113/1‑101 (MQ=255)
         tttGGCGGTCGACAGGCTGGGAACGTGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGCCGCTcc                                                                                             >  1:5929068/1‑101 (MQ=255)
           tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccc                                                                                           >  1:4392155/1‑101 (MQ=255)
             gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                                         <  1:2797691/101‑1 (MQ=255)
                gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgcg                                                                                      >  1:7196538/1‑101 (MQ=255)
                  cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                                                    <  1:7726037/101‑1 (MQ=255)
                   gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgggg                                                                                   >  1:141549/1‑101 (MQ=255)
                     cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                                 <  1:7880159/101‑1 (MQ=255)
                      aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCg                                                                                >  1:450028/1‑101 (MQ=255)
                         gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:5382477/91‑1 (MQ=0)
                         cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:2284555/101‑1 (MQ=255)
                         agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:2442690/91‑1 (MQ=0)
                         aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:2912554/91‑1 (MQ=2)
                          tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            >  2:3785183/1‑101 (MQ=255)
                          aggccggtggtCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  1:5640027/92‑1 (MQ=0)
                           ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  2:3690671/93‑1 (MQ=2)
                           ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  2:594796/93‑1 (MQ=2)
                           ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           >  2:7727392/1‑101 (MQ=255)
                           ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  1:3878431/101‑1 (MQ=255)
                             tagcGTGTCGCTCCCCCCGCTGTGGGGTGGAGCGAGACATC.TCGGTGGCGTCGCGCG.GCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  1:3778071/98‑1 (MQ=25)
                             taccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.GATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  1:4692891/95‑1 (MQ=2)
                              cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        >  2:289991/6‑101 (MQ=2)
                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        >  1:2577310/1‑101 (MQ=255)
                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        <  2:7468184/101‑1 (MQ=255)
                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        >  2:3522281/1‑101 (MQ=255)
                               ggtggccGCGCCCCCCGCGGGCGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:7576541/95‑1 (MQ=2)
                               gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  2:5325716/5‑101 (MQ=2)
                                cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.GATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:6208706/98‑1 (MQ=2)
                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaca                                                                      >  2:4592983/1‑99 (MQ=255)
                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaac                                                                      >  1:1236915/1‑100 (MQ=255)
                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      >  1:6494940/1‑101 (MQ=255)
                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      >  2:295054/1‑101 (MQ=255)
                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      <  1:9048468/101‑1 (MQ=255)
                                 tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     <  2:7884938/99‑1 (MQ=2)
                                 tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     >  2:6099803/3‑101 (MQ=2)
                                 gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     <  1:795459/101‑1 (MQ=255)
                                 gtgtCCCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     <  2:40575/101‑1 (MQ=255)
                                 acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     <  2:5737871/99‑1 (MQ=2)
                                 acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCA.GCCCGTCGCTCCCCCCGCGGGGGCGAGGATCGAAac                                                                     >  1:8112306/3‑101 (MQ=2)
                                 acgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                     <  1:6814963/99‑1 (MQ=2)
                                  ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                                    <  1:4196868/100‑2 (MQ=2)
                                   gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCG.GCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                    <  2:1869087/101‑2 (MQ=2)
                                   gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                                   >  2:6703964/1‑100 (MQ=255)
                                   gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                                   <  1:5491698/101‑3 (MQ=2)
                                   ggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                                   <  1:8868868/99‑1 (MQ=255)
                                    tccgcgcccccGCGGGGGGGGGGGTGGAACCATC.TCGGTGCCGCCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                                  <  2:1419855/95‑1 (MQ=25)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                                  >  1:7298447/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                                  <  1:4928526/101‑4 (MQ=2)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                                  <  1:5863125/101‑4 (MQ=2)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                                  >  2:854082/1‑98 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                                 <  1:3305950/101‑5 (MQ=2)
                                     cGCGCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                                 <  1:3547722/101‑4 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                                >  2:1574000/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                                <  1:2022478/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                                                >  1:8641261/1‑96 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                                <  1:3986629/101‑5 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                                >  2:5132853/1‑97 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                               <  2:556544/101‑7 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                              >  1:3141886/1‑94 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                              >  2:870401/1‑94 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                                              >  2:6335805/1‑95 (MQ=2)
                                        cccccccGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                              <  2:4972082/100‑8 (MQ=2)
                                        cccccccGCGGGGGCGTGGATCAAAACATC.TCGGTCCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                              <  2:3304589/100‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                             >  2:2210904/1‑101 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                             >  1:6147512/1‑94 (MQ=2)
                                             ccGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACACCTTGCGGAt                                                         <  1:9163856/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                                       <  1:7468239/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                                     <  2:2484220/101‑1 (MQ=255)
                                                  ggggCATGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                                    <  2:1264255/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                                   <  1:1690321/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TACAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                                   >  2:4803827/1‑101 (MQ=255)
                                                    ggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                                  <  1:7643225/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                                  <  1:6753060/101‑1 (MQ=255)
                                                     gCGTGGATCGAACCATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                                 <  2:2715316/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                                 >  1:5175997/1‑101 (MQ=255)
                                                      cGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                                <  2:4006789/101‑1 (MQ=255)
                                                      cGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                                <  2:4535872/101‑1 (MQ=255)
                                                        tGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                              >  2:7261399/1‑101 (MQ=255)
                                                            tCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                                          >  2:7974419/1‑101 (MQ=255)
                                                             cGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                                         <  1:5071792/101‑1 (MQ=255)
                                                              gAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                                        >  1:6294451/1‑101 (MQ=255)
                                                               aaaCATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                                       <  1:184963/101‑1 (MQ=255)
                                                                     c.tcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt                                 <  2:6527488/101‑1 (MQ=255)
                                                                       tcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                                <  1:8306242/101‑1 (MQ=255)
                                                                        cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTcc                               >  2:30869/1‑100 (MQ=255)
                                                                         ggTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc                              >  2:6892184/1‑101 (MQ=255)
                                                                            gCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                           <  2:8445413/101‑1 (MQ=255)
                                                                             ccGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc                          >  2:7125232/1‑101 (MQ=255)
                                                                                gcGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc                       <  2:4414072/100‑1 (MQ=255)
                                                                                  gCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc                     >  1:7594083/1‑101 (MQ=255)
                                                                                    tCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg                   >  1:104875/1‑101 (MQ=255)
                                                                                      g.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg                 <  1:2182179/101‑1 (MQ=255)
                                                                                        tCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCggggg                >  2:8229436/1‑101 (MQ=255)
                                                                                         ccAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGTCGCCCCCCCCGCgggggg               >  2:4263767/1‑100 (MQ=255)
                                                                                             ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg           >  2:2038805/1‑101 (MQ=255)
                                                                                              cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGa          <  2:9128206/101‑1 (MQ=255)
                                                                                                g.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:4840342/101‑1 (MQ=255)
                                                                                                    t.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:1737536/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
CATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1395465‑1395665

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG