Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,890 C→T 7.1% intergenic (+702/‑229) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8900C→T 7.1% 79.3 0.0 ‑0.0535intergenic (+702/‑229)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/20):  ref base (237/259):  total (255/280)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 577 total aligned reads displayed.

CGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395790‑1395990
                                                                                                            |                                                                                                       
cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.c                                                                                                         >  2:8139903/1‑101 (MQ=255)
  aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.cgc                                                                                                       <  2:4827365/101‑1 (MQ=255)
    aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.cgcc.g                                                                                                    <  1:6836705/101‑1 (MQ=255)
        gcggggTGAGTCCCCAGTGATAGACGCCGTGGACCTCCCCCCCCCCGCGGGGCAGGGCATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.TCG.Ca                                                                                               <  2:5973596/101‑1 (MQ=255)
        gcggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.Ca                                                                                               >  1:5935295/1‑101 (MQ=255)
             gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGc                                                                                         >  1:7607897/1‑101 (MQ=255)
              tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCt                                                                                        >  1:5588667/1‑101 (MQ=255)
               gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTc                                                                                       >  2:5832761/1‑101 (MQ=255)
               gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTc                                                                                       >  1:2930974/1‑101 (MQ=255)
               gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CGATCGCCTCCGG...TC..GAG.CGCC.GCG.CA.GTGGccc                                                                                       >  1:4560226/1‑99 (MQ=255)
                aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcc                                                                                      >  2:6761381/1‑101 (MQ=255)
                    gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcccccc                                                                                  <  2:3323429/101‑1 (MQ=255)
                    gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcccccc                                                                                  <  2:4682585/101‑1 (MQ=255)
                    gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcccccc                                                                                  <  1:4971427/101‑1 (MQ=255)
                     ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCcg                                                                                 <  2:3773256/101‑1 (MQ=255)
                         tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                                             >  2:7763331/1‑101 (MQ=255)
                         tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                                             >  1:423853/1‑101 (MQ=255)
                          gctAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                                            >  1:5474784/3‑101 (MQ=255)
                          gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCCC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                                            <  1:1221726/101‑1 (MQ=255)
                           aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggggg                                                                           >  2:7974560/1‑101 (MQ=255)
                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                         >  1:832075/1‑101 (MQ=255)
                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                         >  1:1412273/1‑101 (MQ=255)
                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                         >  1:8607255/1‑101 (MQ=255)
                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCg                                                                         >  2:8822694/1‑101 (MQ=255)
                             aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...CC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                         >  2:2747556/1‑101 (MQ=255)
                              gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                                        <  1:563711/101‑1 (MQ=255)
                                 gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:3557867/1‑101 (MQ=255)
                                 gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:6275556/101‑1 (MQ=255)
                                 cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                                        >  1:514542/9‑101 (MQ=2)
                                  caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:8312240/93‑1 (MQ=9)
                                   cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGGGGATc                                                                   >  2:4206457/1‑101 (MQ=255)
                                   aacctatgTCGCGCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:1233093/94‑1 (MQ=9)
                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  2:1767133/1‑101 (MQ=255)
                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:2075981/101‑1 (MQ=255)
                                    cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  2:2545548/6‑101 (MQ=0)
                                    acctatgTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:6967103/95‑1 (MQ=9)
                                    acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGGGGATCg                                                                  >  2:7689923/7‑101 (MQ=9)
                                     cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC..GAG.CGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:4870085/97‑1 (MQ=2)
                                      ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  1:3759071/1‑101 (MQ=255)
                                      gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC..GAG.CGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:1003239/98‑1 (MQ=2)
                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  1:6130010/5‑101 (MQ=9)
                                      cggcctCGCTCCCCCGCGGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:7357145/96‑1 (MQ=2)
                                      cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:3355431/98‑1 (MQ=2)
                                       ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:5409195/99‑1 (MQ=2)
                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  2:5277187/2‑101 (MQ=2)
                                        aCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  1:8080304/1‑101 (MQ=255)
                                        aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  2:2230836/101‑1 (MQ=255)
                                         tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CTGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                             <  1:6569623/100‑2 (MQ=2)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                            >  2:3092871/1‑101 (MQ=255)
                                           tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                           <  2:1896981/101‑1 (MQ=9)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                          >  2:295976/1‑101 (MQ=255)
                                             gttgcggcggcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                         >  2:5622341/10‑101 (MQ=2)
                                             gCTCCCCGCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                         <  1:1648682/101‑1 (MQ=255)
                                             gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                         >  1:2618497/1‑101 (MQ=9)
                                             gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                         <  2:1290315/101‑1 (MQ=9)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGG...TCTTCTGTTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGGGTGGGTCGagcaccc                                                           >  1:8942870/1‑95 (MQ=0)
                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACAATGCg                                                        >  2:4035197/1‑101 (MQ=9)
                                               tcgccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                       <  1:3137533/98‑2 (MQ=9)
                                               tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                       <  2:3487612/98‑2 (MQ=9)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                       >  1:7898144/1‑101 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTTCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                      <  2:5102342/101‑1 (MQ=255)
                                                caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                      >  2:1498702/3‑99 (MQ=9)
                                                 cccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                     <  1:1940832/101‑4 (MQ=9)
                                                 accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                     >  2:8566572/2‑98 (MQ=9)
                                                 accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                     >  1:8700168/2‑98 (MQ=9)
                                                  ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                    <  2:8999403/101‑1 (MQ=255)
                                                    ccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                                  <  2:1007300/101‑7 (MQ=2)
                                                       cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                               <  2:6633461/101‑1 (MQ=255)
                                                        gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggtcct                                              <  1:7210968/101‑11 (MQ=2)
                                                        gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                              >  1:3905723/1‑101 (MQ=255)
                                                        gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                              >  2:635560/1‑101 (MQ=255)
                                                         ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                             >  1:4401505/1‑101 (MQ=255)
                                                          gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                            >  2:6967809/1‑101 (MQ=255)
                                                              gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                        >  1:5345701/1‑101 (MQ=255)
                                                              gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                        >  2:2088928/1‑101 (MQ=255)
                                                              gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                        >  1:7419266/1‑101 (MQ=255)
                                                              gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                        >  2:6441795/1‑101 (MQ=255)
                                                               tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                       >  2:4382781/1‑101 (MQ=255)
                                                                ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                      >  1:4645542/1‑101 (MQ=255)
                                                                  aTCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGATGCGTTCATGGCCTACGTCGGGcg                                    <  1:6446786/101‑2 (MQ=255)
                                                                    cGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                  >  2:8506744/1‑101 (MQ=255)
                                                                    cGAAACTGCG.CCGC.CTATCGCATCCGG...GA..GGG.CGCC.GCG.CA.TTCGCTCCCCCCGCGGGGGCGTGGATGGAGACGATCCGCTCCTCCCCCACGGCGGGCAgg                                  >  1:9099135/1‑100 (MQ=19)
                                                                     gAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                 >  2:5500932/1‑101 (MQ=255)
                                                                     gAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                 <  1:30869/101‑1 (MQ=255)
                                                                     gAAACGGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                 <  2:6139891/101‑1 (MQ=255)
                                                                           gcg.cCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                           <  2:7138080/101‑1 (MQ=255)
                                                                           gcg.cCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                           <  1:2747638/101‑1 (MQ=255)
                                                                            cg.ccgc.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                          <  1:1812374/101‑1 (MQ=255)
                                                                                    cTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                    >  2:4915939/1‑101 (MQ=255)
                                                                                      aTCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                  >  1:673558/1‑101 (MQ=255)
                                                                                        cGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                >  1:7811835/1‑101 (MQ=255)
                                                                                           aTCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg             >  1:2292651/1‑101 (MQ=255)
                                                                                           aTCCGG...TC..GAG.CCCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCcgcg             >  1:6655809/1‑101 (MQ=255)
                                                                                             ccGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg           >  1:8407556/1‑101 (MQ=255)
                                                                                              cGG...TC..GAG.CCCC.CCG.GA.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg          <  2:2397569/101‑1 (MQ=38)
                                                                                               gg...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg         >  1:4078010/1‑101 (MQ=255)
                                                                                               gg...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg         >  2:2678779/1‑101 (MQ=255)
                                                                                                     c..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg      >  2:6477376/1‑100 (MQ=255)
                                                                                                     c..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt      >  1:629841/1‑101 (MQ=255)
                                                                                                         aG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg    >  1:3675910/1‑101 (MQ=255)
                                                                                                            |                                                                                                       
CGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395790‑1395990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG