Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,553 T→C 16.5% T145T (ACT→AGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5530T→C16.5% 192.3 ‑1.9 ‑0.5496T145T (ACT→ACCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (47/35):  ref base (200/214):  total (247/249)
Fisher's exact test strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.00e-01

Only 100 of 513 total aligned reads displayed.

GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653
                                                                                                     |                                                                                                    
  ggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTgg                                                                                                    <  2:1896050/101‑1 (MQ=255)
    cGTGGAGCGAAACGGACCTGAACTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGcc                                                                                                  <  2:4228820/101‑1 (MQ=255)
     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                 <  1:7057643/101‑1 (MQ=255)
     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                 >  1:611928/1‑101 (MQ=255)
     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                 >  1:7675575/1‑101 (MQ=255)
       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                               >  1:3097025/1‑101 (MQ=255)
        gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                              <  1:1749088/101‑1 (MQ=255)
         aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                             <  1:3868307/101‑1 (MQ=255)
           cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgcc                                                                                           >  1:4279617/1‑101 (MQ=255)
           cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgcc                                                                                           <  1:4197814/101‑1 (MQ=255)
            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                                          <  1:7296727/101‑1 (MQ=255)
              aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                        >  1:4089767/1‑101 (MQ=255)
              aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                        <  1:1072603/101‑1 (MQ=255)
               aCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCcgcg                                                                                       >  2:2136699/1‑101 (MQ=255)
                cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCCgcgc                                                                                      >  2:4131479/1‑101 (MQ=255)
                 ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                     <  1:5212291/101‑1 (MQ=255)
                 ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                     >  1:7159009/1‑101 (MQ=255)
                  gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTGGTTTC.GTGGGAGTGGCCAGCCGCCCCGGCCa                                                                                    >  1:8929864/1‑101 (MQ=38)
                      tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                <  1:2243949/101‑1 (MQ=255)
                              cgctgtaaccgcgGCGGGGAGCGCGTCTCGCCCCCCGCGGGCGCGTGGATCGAAACTGCTTTC.GTGGGACCGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                        <  2:4676613/92‑1 (MQ=37)
                               gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                       <  2:2631507/101‑1 (MQ=255)
                               gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                       <  2:14897/101‑1 (MQ=255)
                                cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                      <  1:8662736/101‑1 (MQ=255)
                                cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                      <  2:5350913/101‑1 (MQ=255)
                                  ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                    >  1:2859991/1‑101 (MQ=255)
                                  ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                    >  2:5567859/1‑101 (MQ=255)
                                     aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                 >  2:3488726/1‑101 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               <  2:6407201/101‑1 (MQ=255)
                                         cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGt                                                             <  1:1948431/101‑1 (MQ=255)
                                             tgggacggagTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                          >  1:6630440/10‑101 (MQ=2)
                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         >  2:3898764/9‑101 (MQ=2)
                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         >  2:5912946/9‑101 (MQ=2)
                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         <  2:836153/93‑1 (MQ=2)
                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGAt                                                         >  2:4879543/9‑101 (MQ=2)
                                              gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:2016439/101‑1 (MQ=255)
                                              gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:9184486/101‑1 (MQ=255)
                                               ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                        <  2:3626180/94‑1 (MQ=2)
                                               cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:5161188/101‑1 (MQ=255)
                                               cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:8373575/101‑1 (MQ=255)
                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  2:4389387/1‑101 (MQ=255)
                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  2:7269439/1‑101 (MQ=255)
                                                 acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                      >  2:2381254/6‑101 (MQ=2)
                                                 aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:6237506/101‑1 (MQ=255)
                                                 aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:2891993/101‑1 (MQ=255)
                                                  gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:6319153/1‑101 (MQ=255)
                                                   ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                    >  2:4458350/4‑101 (MQ=2)
                                                    gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:5064354/99‑1 (MQ=2)
                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                 >  1:6674762/1‑101 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACCGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                <  2:5227339/101‑1 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTA.GTGGGACTGGCCAGCCGCCCGAGCCAAGCCGCTCCCCCACGGGGGGCGGGGTTCGAGACGac                                                >  2:684679/1‑100 (MQ=25)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                               <  1:6013524/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             <  1:325900/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             >  2:2273073/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                              <  1:6434925/101‑6 (MQ=2)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGGCGCTCCCCCCGCCGGGGCGTGGATCGAAACCGTTtcg                                            >  2:5055103/1‑101 (MQ=25)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                            <  1:4498209/101‑8 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          <  1:5534766/101‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          >  1:3590722/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           >  2:81110/1‑93 (MQ=2)
                                                              ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                         <  1:8281990/101‑11 (MQ=2)
                                                                ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                      <  1:7758835/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                     <  1:1824970/101‑1 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  1:2127086/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                   >  1:7826840/1‑101 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                   <  2:1172326/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTTTCGTCGCTTaccg                                  >  2:8665506/1‑99 (MQ=255)
                                                                     ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                 <  2:6674502/101‑1 (MQ=255)
                                                                     ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                 <  2:5430801/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                <  1:4968739/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                <  2:7604033/101‑1 (MQ=255)
                                                                         cttGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                             >  1:4720745/3‑101 (MQ=255)
                                                                         cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                             >  1:4070500/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGTTCGCAACGATCTCGTCGCTTACGACGCCTa                            >  1:9048976/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                            >  1:1645361/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                            >  2:794984/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                            >  1:5756623/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                            >  2:2744469/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           >  2:1713771/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           >  1:853048/1‑101 (MQ=255)
                                                                           gggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           <  2:2473886/100‑1 (MQ=255)
                                                                            ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                          <  1:3331626/101‑1 (MQ=255)
                                                                            ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                          >  1:2386764/1‑101 (MQ=255)
                                                                             gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         <  1:6759117/101‑1 (MQ=255)
                                                                               tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                       >  2:7094332/1‑101 (MQ=255)
                                                                                  aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    >  1:5437242/1‑101 (MQ=255)
                                                                                  aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    >  2:5651656/1‑101 (MQ=255)
                                                                                  aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    >  1:4868024/1‑101 (MQ=255)
                                                                                     cTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                 <  2:5496250/101‑1 (MQ=255)
                                                                                      tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                >  1:809003/1‑101 (MQ=255)
                                                                                        cTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc              >  2:1783587/1‑101 (MQ=255)
                                                                                        cTTTC.GGGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc              <  2:3223857/101‑1 (MQ=255)
                                                                                            c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTTTCGTCGCTTACGACGCCTATCTGATTTCGGGGCGCTc          >  1:1368578/1‑101 (MQ=255)
                                                                                            c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  2:7191862/1‑101 (MQ=255)
                                                                                            c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  2:1042352/1‑101 (MQ=255)
                                                                                            c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  2:8854292/1‑101 (MQ=255)
                                                                                              gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc         >  1:11379/1‑101 (MQ=255)
                                                                                                gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc       <  1:7927954/101‑1 (MQ=255)
                                                                                                 ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc      <  1:7395861/101‑1 (MQ=255)
                                                                                                   aCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg    <  1:2623682/101‑1 (MQ=255)
                                                                                                    cTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc   >  1:3533386/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG