Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,803 C→G 16.3% R229G (CGT→GGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8030C→G16.3% 243.4 ‑4.2 0.0601R229G (CGT→GGT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (36/61):  ref base (255/246):  total (292/309)
Fisher's exact test strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 640 total aligned reads displayed.

ATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACG  >  NC_014034/1396703‑1396903
                                                                                                    |                                                                                                       
  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgt                                                                                                       >  2:6986425/1‑101 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTgg                                                                                                   <  1:3993675/101‑1 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTgg                                                                                                   <  1:8291625/101‑1 (MQ=255)
         ccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...g                                                                                             <  2:8187886/101‑1 (MQ=255)
            cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATc                                                                                          <  1:8665891/101‑1 (MQ=255)
            cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATc                                                                                          <  1:4831313/101‑1 (MQ=255)
               gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                       <  2:4070385/101‑1 (MQ=255)
                 gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                     >  1:6715984/1‑101 (MQ=255)
                   gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                   >  2:8640483/1‑101 (MQ=255)
                   gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                   <  1:2677464/101‑1 (MQ=255)
                       gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCgg                                                                               <  2:3481944/101‑1 (MQ=255)
                        tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                              >  1:3127723/1‑101 (MQ=255)
                         ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                             >  2:4981388/1‑101 (MQ=255)
                         ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                             <  2:4209403/101‑1 (MQ=255)
                         ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                             >  2:6400937/1‑101 (MQ=255)
                          gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                            >  1:4258983/1‑101 (MQ=255)
                          gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                            <  2:7681507/101‑1 (MQ=255)
                            tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                          >  2:4828278/1‑101 (MQ=255)
                             cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                         >  2:120176/1‑101 (MQ=255)
                             cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                         >  1:1908922/1‑101 (MQ=255)
                             cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                         >  1:1599416/1‑101 (MQ=255)
                              gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                        >  2:29403/1‑101 (MQ=255)
                               aaccccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                       <  2:1450270/98‑1 (MQ=255)
                               aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                       >  2:7076301/1‑101 (MQ=255)
                               aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                       <  2:3827238/101‑1 (MQ=255)
                                aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTgg                                                                      <  2:5437057/101‑1 (MQ=255)
                                aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTgg                                                                      <  1:5055272/101‑1 (MQ=255)
                                  cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGTGt                                                                    <  2:841242/101‑1 (MQ=255)
                                  cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGt                                                                    >  1:2295179/1‑101 (MQ=255)
                                   ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                   <  1:9138684/101‑1 (MQ=255)
                                       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                               <  1:379866/101‑1 (MQ=255)
                                       tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                               >  2:6826362/1‑101 (MQ=255)
                                        cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                              >  2:3162653/1‑101 (MQ=255)
                                            cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                          <  2:2640684/101‑1 (MQ=255)
                                            cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                          <  1:6173443/101‑1 (MQ=255)
                                            cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCGcccccc                                                          >  2:5555121/1‑101 (MQ=255)
                                              tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                        >  1:7914910/1‑101 (MQ=255)
                                               atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgcg                                                       >  1:5202220/1‑101 (MQ=255)
                                                  ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                    <  1:7103245/101‑1 (MQ=255)
                                                       aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:647141/1‑101 (MQ=255)
                                                       aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:9053912/1‑101 (MQ=255)
                                                        cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTGGCTTCCCCCGCGGGGGGGTgg                                              >  2:7365099/1‑101 (MQ=255)
                                                        cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:7636366/101‑1 (MQ=255)
                                                         gATGCCCTTGAGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:7213715/101‑1 (MQ=255)
                                                         gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:8498538/1‑101 (MQ=255)
                                                         gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:1076605/1‑101 (MQ=255)
                                                          aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:5421848/101‑1 (MQ=255)
                                                           tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:8886611/101‑1 (MQ=255)
                                                           cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:5705018/10‑101 (MQ=0)
                                                            gctcTTGAAGCGCTCCCGCCGCGGGGGCGTGGATCGAAACCGTGTAGAT...GATCATGCAGGGCGGACGGCTTGGGCCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:7834293/98‑1 (MQ=37)
                                                            acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:4079349/93‑1 (MQ=2)
                                                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:1003452/1‑101 (MQ=255)
                                                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:239621/101‑1 (MQ=255)
                                                              ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:8440214/101‑1 (MQ=255)
                                                              atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:2526524/95‑1 (MQ=2)
                                                               cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:3344141/1‑101 (MQ=255)
                                                               cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCGGGGGCGTgggtacaaa                                       >  1:2935687/1‑94 (MQ=255)
                                                                ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  2:8832301/101‑1 (MQ=255)
                                                                 gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:7486285/98‑1 (MQ=2)
                                                                 gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:2166426/98‑1 (MQ=2)
                                                                  gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                    <  2:1970552/101‑1 (MQ=255)
                                                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATATCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                     >  2:4361382/2‑100 (MQ=2)
                                                                   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                   >  1:8531433/1‑101 (MQ=255)
                                                                   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                   <  1:5587412/101‑1 (MQ=255)
                                                                   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGCGGGTCGAAACCgg                                   >  1:6315745/1‑101 (MQ=255)
                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                    >  2:6708970/1‑99 (MQ=2)
                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCggc                                  >  2:1584597/1‑100 (MQ=255)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                 <  1:1796794/101‑1 (MQ=255)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcattg                                  >  2:4575125/1‑97 (MQ=2)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                >  2:1713710/1‑101 (MQ=255)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                >  1:6898420/1‑101 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                <  2:8247508/101‑7 (MQ=2)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCag                              >  2:5255120/1‑101 (MQ=255)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                               >  1:4691928/1‑94 (MQ=2)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                               >  2:6584580/1‑94 (MQ=2)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                             <  1:4485285/101‑1 (MQ=255)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                             >  2:6187571/1‑101 (MQ=255)
                                                                         cccgtccGCGGGGGCGTGGATCGAACCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                             <  2:5820333/96‑1 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                              <  2:5151443/101‑9 (MQ=2)
                                                                          ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                            >  1:2426677/1‑101 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                            <  2:7177908/101‑1 (MQ=255)
                                                                            ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                            >  1:3696075/1‑91 (MQ=0)
                                                                                gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGCGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                      <  1:6986710/101‑1 (MQ=255)
                                                                                 cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                     >  1:5789863/1‑101 (MQ=255)
                                                                                        gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa              >  2:8061062/1‑101 (MQ=255)
                                                                                         tGGATCGAAACCATGTGGAT...GATCATGCGGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCAGGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc             >  1:6520135/1‑101 (MQ=38)
                                                                                          ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc            >  2:7860784/1‑101 (MQ=255)
                                                                                          ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc            >  1:3294082/1‑101 (MQ=255)
                                                                                            aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCACGGAcctc          <  1:7415117/101‑1 (MQ=255)
                                                                                             tCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCACAGATAGACCTCCCTGAcctcc         >  2:5986194/1‑101 (MQ=37)
                                                                                              cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct        <  2:6276078/101‑1 (MQ=255)
                                                                                              cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct        <  1:1186055/101‑1 (MQ=255)
                                                                                                aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa      <  2:7539716/101‑1 (MQ=255)
                                                                                                aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa      >  1:5999722/1‑101 (MQ=255)
                                                                                                aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa      >  2:3685700/1‑101 (MQ=255)
                                                                                                 aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  1:1399051/1‑101 (MQ=255)
                                                                                                 aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg     >  2:1843041/1‑101 (MQ=255)
                                                                                                   ccGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc   <  1:3533257/101‑1 (MQ=255)
                                                                                                   ccGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc   <  1:6626512/101‑1 (MQ=255)
                                                                                                    cGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg  >  1:2064408/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
ATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACG  >  NC_014034/1396703‑1396903

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG