Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,872 C→T 1.1% intergenic (+176/+1722) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8720C→T 1.1% 3.3 ‑1.0 ‑0.8465intergenic (+176/+1722)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (215/244):  total (217/248)
Fisher's exact test strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.52e-01

Only 100 of 494 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGT  >  NC_014034/1397772‑1397972
                                                                                                    |                                                                                                    
ccccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                                                                                      >  1:5163463/1‑101 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCAc                                                                                                    <  1:7506321/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTc                                                                                                 <  1:2832499/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCa                                                                                                <  1:7779066/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCa                                                                                                <  2:4715596/101‑1 (MQ=255)
       cgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAg                                                                                               >  1:8539129/1‑101 (MQ=255)
        gggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGc                                                                                              >  2:7802123/1‑101 (MQ=255)
        gggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGc                                                                                              >  2:6412456/1‑101 (MQ=255)
        gggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGc                                                                                              >  1:630908/1‑101 (MQ=255)
          gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGccc                                                                                            >  1:2316544/1‑101 (MQ=255)
          gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCGGAACCTCAGccc                                                                                            >  2:2570553/1‑101 (MQ=255)
            gCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccg                                                                                          <  2:5588254/101‑1 (MQ=255)
              gTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgcc                                                                                        >  1:4875846/1‑101 (MQ=255)
                ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgcccg                                                                                      >  2:793748/1‑101 (MQ=255)
                  aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCt                                                                                    <  2:5834370/101‑1 (MQ=255)
                    cGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCCGGCACCTCAGCCCCGCCCGCTGt                                                                                  >  1:4667401/1‑101 (MQ=255)
                      aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccggccttgacac                                                                                <  2:8639562/101‑8 (MQ=255)
                      aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtct                                                                                >  1:4476699/1‑101 (MQ=255)
                       aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                               <  1:5540007/101‑1 (MQ=255)
                       aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                               <  2:3623827/101‑1 (MQ=255)
                       aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                               <  2:9188871/101‑1 (MQ=255)
                         cGGTGTCCAGCGTCACATGCAGCGGCNNNNNATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAc                                                                             <  1:978171/101‑1 (MQ=255)
                         cGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAc                                                                             <  2:5098679/101‑1 (MQ=255)
                          ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAcc                                                                            >  1:6120755/1‑101 (MQ=255)
                             gtCCAGCGTCACATGCAGCGGCGTCGCATCGCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                         <  2:6760022/101‑1 (MQ=255)
                             gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                         >  2:3033715/1‑101 (MQ=255)
                             gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                         <  1:1037975/101‑1 (MQ=255)
                              tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                        <  1:7076793/101‑1 (MQ=255)
                                cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                      <  1:1628848/101‑1 (MQ=255)
                                  gCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCg                                                                    <  2:478063/101‑1 (MQ=255)
                                  gCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCg                                                                    <  1:7867918/101‑1 (MQ=255)
                                   cggcacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                   <  2:1189135/98‑1 (MQ=255)
                                   cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                   >  2:5235919/1‑101 (MQ=255)
                                    gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                  >  2:2463930/1‑101 (MQ=255)
                                     tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCGGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                 <  2:1403549/101‑1 (MQ=255)
                                     gcacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                 <  2:630898/100‑1 (MQ=255)
                                      cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                                <  1:8279904/101‑1 (MQ=255)
                                       acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                               <  1:123182/101‑1 (MQ=255)
                                        caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                              >  2:2661229/1‑101 (MQ=255)
                                         aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                             >  1:7416015/1‑101 (MQ=255)
                                         aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                             <  2:8508305/101‑1 (MQ=255)
                                          tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                            <  1:2738612/101‑1 (MQ=255)
                                           gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                           >  2:7519113/1‑101 (MQ=255)
                                           gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                           <  1:4282823/101‑1 (MQ=255)
                                            cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                          <  2:3874729/101‑1 (MQ=255)
                                             aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                         >  1:6937492/1‑101 (MQ=255)
                                                   gTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCg                                                   >  2:7010691/1‑101 (MQ=255)
                                                    tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                  <  1:3454879/101‑1 (MQ=255)
                                                     cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                 >  1:6878221/1‑101 (MQ=255)
                                                         tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  2:2316893/1‑101 (MQ=255)
                                                           gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  1:5853301/1‑101 (MQ=255)
                                                            cgatctCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:3202012/96‑1 (MQ=255)
                                                            ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:8261097/101‑1 (MQ=255)
                                                            ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  1:2103676/1‑101 (MQ=255)
                                                             cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  2:4116673/1‑101 (MQ=255)
                                                              aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        <  2:9027780/101‑1 (MQ=255)
                                                              aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:8101526/1‑101 (MQ=255)
                                                               tctggCTCCCCCCGCGGGGGCGTGGATCGAAGCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                       <  2:4263575/97‑1 (MQ=255)
                                                               tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                       >  1:6703351/1‑100 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                      <  1:4172024/101‑1 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                      >  2:1764683/1‑101 (MQ=255)
                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                      <  1:1518684/101‑1 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                     >  2:8802439/1‑101 (MQ=255)
                                                                 ccGCGCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                     <  2:7601201/100‑1 (MQ=255)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                   <  1:4744252/101‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                  <  2:7224786/101‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                  <  2:3428624/101‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACTCTATgg                                 >  2:2250440/1‑101 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                <  1:8962117/101‑1 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                               <  1:3245987/101‑1 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                             <  2:4333387/101‑1 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                             >  1:3919671/1‑101 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                            <  1:2480450/101‑1 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                            <  2:2067414/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACTATgggaggct                           <  1:6532273/101‑1 (MQ=255)
                                                                              gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                        <  1:3033814/101‑1 (MQ=255)
                                                                              gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                        >  1:727807/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                      <  2:7415691/101‑1 (MQ=255)
                                                                                gggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                      >  2:1957973/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                      >  1:1886537/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                    <  1:8106079/101‑1 (MQ=255)
                                                                                   cGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                   >  2:6578512/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                  <  2:1316266/101‑1 (MQ=255)
                                                                                     tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                 >  1:5701167/1‑101 (MQ=255)
                                                                                     tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                 >  1:2202/1‑101 (MQ=255)
                                                                                      ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                <  2:6531889/101‑1 (MQ=255)
                                                                                      ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                >  1:8352985/1‑101 (MQ=255)
                                                                                       gATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg               <  2:5414536/101‑1 (MQ=255)
                                                                                        aTCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc              <  2:1665953/101‑1 (MQ=255)
                                                                                        aTCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc              <  2:8352622/101‑1 (MQ=255)
                                                                                          cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc            >  1:3686617/1‑101 (MQ=255)
                                                                                           gAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc           >  2:2823363/1‑101 (MQ=255)
                                                                                             aaCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt         <  1:3813161/101‑1 (MQ=255)
                                                                                                ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa      >  2:6193141/1‑101 (MQ=255)
                                                                                                 cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGCGGCTGGGGAGGCTACGCACCTTCaaa     >  2:4914264/1‑101 (MQ=255)
                                                                                                 cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa     <  2:4575106/101‑1 (MQ=255)
                                                                                                  ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa    >  2:56562/1‑101 (MQ=255)
                                                                                                   gCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg   <  2:4460628/101‑1 (MQ=255)
                                                                                                    cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt  >  1:915103/1‑101 (MQ=255)
                                                                                                    cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt  >  1:5052723/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGT  >  NC_014034/1397772‑1397972

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG