Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,398 A→T 0.9% intergenic (‑177/‑781) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3980A→T 0.9% 3.6 0.0 ‑0.1530intergenic (‑177/‑781)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (230/293):  total (233/297)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.51e-01

Only 100 of 553 total aligned reads displayed.

CTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGG  >  NC_014034/1434298‑1434496
                                                                                                        |                                                                                                       
cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCa                                                                                                         >  2:8246442/1‑101 (MQ=255)
cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCa                                                                                                         >  1:3275012/1‑101 (MQ=255)
 ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                                        <  1:4828454/101‑1 (MQ=255)
 ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                                        >  2:4948463/1‑101 (MQ=255)
   aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACg.g                                                                                                     >  2:2403599/1‑101 (MQ=255)
    ctgTGGATCGAACATTGCTGCCTTGGTCGGAACACCTTATCCTTCTTGGTTGCTCCCCCCGCGGGTGCGTTGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.Gc                                                                                                    <  2:5900351/99‑1 (MQ=255)
     cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                                   >  1:7597707/1‑101 (MQ=255)
     cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                                   >  2:5397679/1‑101 (MQ=255)
        ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCTCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.Cg                                                                                               >  1:3371110/1‑101 (MQ=255)
           tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTcc                                                                                            >  2:4489226/1‑100 (MQ=255)
            cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCaa                                                                                           >  2:1041980/1‑101 (MQ=255)
             gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                          >  1:6302128/1‑101 (MQ=255)
              aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                                         >  2:6597241/1‑101 (MQ=255)
              aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGATCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                                         >  2:6086804/1‑101 (MQ=255)
               aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                                        <  2:1771513/101‑1 (MQ=255)
                aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCCCG.GCTG.CGTCAACCtt                                                                                       >  1:6568961/1‑101 (MQ=255)
                    gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.Cg                                                                                >  1:2022791/1‑101 (MQ=255)
                       gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTc                                                                             >  2:7051810/1‑101 (MQ=255)
                          ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTcccc                                                                          <  2:8429031/101‑1 (MQ=255)
                           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTccccc                                                                         <  1:4749334/101‑1 (MQ=255)
                            gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTcccccc                                                                        <  1:1425989/101‑1 (MQ=255)
                             ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCcg                                                                       >  2:7587597/1‑101 (MQ=255)
                             ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCcg                                                                       >  2:4052354/1‑101 (MQ=255)
                               cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCcccg                                                                     >  2:1318303/1‑101 (MQ=255)
                                ggAACGCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCgg                                                                    <  2:5708825/101‑1 (MQ=255)
                                 gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCggg                                                                   >  2:5587558/1‑101 (MQ=255)
                                 gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCggg                                                                   >  1:9083024/1‑101 (MQ=255)
                                  aaCACCTGAGCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCgggg                                                                  <  2:6939275/101‑1 (MQ=255)
                                     acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCg                                                               >  2:3929476/1‑101 (MQ=255)
                                     acCAGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCggggggg                                                               >  1:7213238/1‑99 (MQ=255)
                                       cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTg                                                             <  1:2134545/101‑1 (MQ=255)
                                          aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGac                                                          >  2:225014/1‑100 (MQ=255)
                                           tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:5893786/101‑1 (MQ=255)
                                           tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:2474972/101‑1 (MQ=255)
                                            ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  1:4335561/101‑1 (MQ=255)
                                            ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:4788773/101‑1 (MQ=255)
                                            ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                        >  2:3713093/1‑101 (MQ=255)
                                             ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGa                                                       >  2:9182526/1‑101 (MQ=255)
                                              tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:5260915/101‑1 (MQ=255)
                                              gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGGTc                                                         >  1:4488511/7‑101 (MQ=2)
                                              gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:7885961/95‑1 (MQ=2)
                                              gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:2150852/95‑1 (MQ=2)
                                               ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                        >  2:4409295/6‑101 (MQ=2)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGACAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   <  2:6752980/101‑1 (MQ=255)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   >  1:8311215/1‑101 (MQ=255)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   >  2:8767354/1‑101 (MQ=255)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCCCCGGGGCGCGGCTAGAAaca                                                   >  2:6262971/1‑101 (MQ=37)
                                                  tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:3034259/3‑101 (MQ=2)
                                                  tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  1:3545041/99‑1 (MQ=2)
                                                  gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                  <  1:3769151/101‑1 (MQ=255)
                                                   ggTCGCTCCCCCCGCGGGGGCGCGGATCGAAAC..GTCTCCT.CTCTG.CTTCACG.GCTG.CGACAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                 <  1:1606295/101‑1 (MQ=255)
                                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                    >  1:7677790/2‑101 (MQ=2)
                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                <  1:7824828/101‑1 (MQ=255)
                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                   >  2:184771/1‑100 (MQ=2)
                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                               >  1:1882574/1‑101 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGNAAC..NTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                              <  1:5788804/101‑1 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGGGGATCGAAaccct                                                 >  1:1212164/1‑98 (MQ=2)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                             >  2:79879/1‑101 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                             <  2:4584378/101‑1 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                             <  2:8846213/101‑1 (MQ=255)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                               <  1:6906154/101‑6 (MQ=2)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                              >  1:5579428/1‑95 (MQ=2)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                           >  2:3326647/1‑101 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             >  1:4967330/1‑94 (MQ=2)
                                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             <  2:7238310/101‑8 (MQ=2)
                                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             <  1:7427006/101‑8 (MQ=2)
                                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             <  2:5301059/101‑8 (MQ=2)
                                                          ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                          >  1:7881878/1‑101 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCC.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                          <  1:6265211/101‑1 (MQ=255)
                                                           cccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                            <  2:9042635/101‑9 (MQ=2)
                                                           cccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                         <  2:2895605/101‑1 (MQ=255)
                                                            ccccGCGGGGGCGTGGATCGAAAC..TTGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaacgac                                         <  1:8791791/101‑8 (MQ=37)
                                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                   >  2:3057753/1‑101 (MQ=255)
                                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                   >  1:7052891/1‑101 (MQ=255)
                                                                 cgGGCGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                   <  2:3536740/101‑1 (MQ=255)
                                                                  ctgggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                  <  2:7678107/99‑1 (MQ=255)
                                                                    gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCgcg                                >  2:1611077/1‑99 (MQ=255)
                                                                     ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                               <  1:2734648/101‑1 (MQ=255)
                                                                     ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                               <  1:5414358/101‑1 (MQ=255)
                                                                       cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                             >  2:2733843/1‑101 (MQ=255)
                                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                           <  2:2331908/101‑1 (MQ=255)
                                                                          ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                          <  1:604482/101‑1 (MQ=255)
                                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                      >  1:107803/1‑101 (MQ=255)
                                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                     <  1:8177462/101‑1 (MQ=255)
                                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                     >  2:1476317/1‑101 (MQ=255)
                                                                               gAAAC..GGCTCCG.CTCTT.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                     <  1:8696868/101‑1 (MQ=255)
                                                                               gAAAC..GGCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                     <  2:1110265/101‑1 (MQ=255)
                                                                                aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                    >  2:3225394/1‑101 (MQ=255)
                                                                                aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                    >  1:6346989/1‑101 (MQ=255)
                                                                                   c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                 <  1:848887/101‑1 (MQ=255)
                                                                                       tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc               <  1:4099684/101‑1 (MQ=255)
                                                                                       tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc               >  2:6479084/1‑101 (MQ=255)
                                                                                        ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg              >  2:6350214/1‑101 (MQ=255)
                                                                                        ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg              <  1:4519346/101‑1 (MQ=255)
                                                                                           cG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc           <  2:1972192/101‑1 (MQ=255)
                                                                                            g.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGCCAGGCTTTGCAggacgtcc          >  1:4017369/1‑94 (MQ=255)
                                                                                              ctctG.CTTCACG.GCTG.CGTCAACCTT..GTCCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc          <  1:8941057/101‑1 (MQ=255)
                                                                                               tctG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc        >  2:7315799/1‑101 (MQ=255)
                                                                                                ctG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc       <  2:8570103/101‑1 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GT.CGCCCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg     <  2:5147257/101‑1 (MQ=255)
                                                                                                        |                                                                                                       
CTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGG  >  NC_014034/1434298‑1434496

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG