Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,409 A→T 20.8% intergenic (‑188/‑770) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4090A→T20.8% 0.0 ‑0.8 ‑0.6519intergenic (‑188/‑770)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (50/58):  ref base (173/237):  total (223/296)
Fisher's exact test strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.49e-01

Only 100 of 548 total aligned reads displayed.

TCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1434309‑1434509
                                                                                                       |                                                                                                       
 cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                                                        >  2:1041980/1‑101 (MQ=255)
   aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                                      >  2:6597241/1‑101 (MQ=255)
     aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                    <  2:7867632/101‑1 (MQ=255)
      cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 <  1:1976671/101‑1 (MQ=255)
           tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCt                                                                                           <  2:4574614/101‑1 (MQ=255)
           tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCt                                                                                           <  2:2307424/101‑1 (MQ=255)
            gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTc                                                                                          >  2:7051810/1‑101 (MQ=255)
             ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTcc                                                                                         >  1:3455528/1‑101 (MQ=255)
               ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTGTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTcccc                                                                                       <  1:8199213/101‑1 (MQ=255)
                tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTccccc                                                                                      <  1:6802963/101‑1 (MQ=255)
                  ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCcg                                                                                    <  2:3967990/101‑1 (MQ=255)
                      gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCggg                                                                                >  1:9083024/1‑101 (MQ=255)
                        acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCggggg                                                                              >  1:6464498/1‑101 (MQ=255)
                        acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCCCggggg                                                                              >  1:7946521/1‑101 (MQ=255)
                         cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGc                                                                             <  1:1370762/101‑1 (MQ=255)
                         cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGc                                                                             <  1:4806621/101‑1 (MQ=255)
                              gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGa                                                                        <  1:2299263/101‑1 (MQ=255)
                               aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:6108467/101‑1 (MQ=255)
                               aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:1912120/1‑101 (MQ=255)
                                cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:1729939/92‑1 (MQ=2)
                                cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:3512227/92‑1 (MQ=2)
                                cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:2351921/92‑1 (MQ=2)
                                cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:1142919/92‑1 (MQ=2)
                                 ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  2:4788773/101‑1 (MQ=255)
                                 ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:4491071/101‑1 (MQ=255)
                                  ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  2:9182526/1‑101 (MQ=255)
                                   gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  2:3689168/7‑101 (MQ=2)
                                   gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  1:2038027/7‑101 (MQ=2)
                                    ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:178397/96‑1 (MQ=2)
                                    ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  2:1204716/96‑1 (MQ=2)
                                     tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  1:8486400/97‑1 (MQ=2)
                                     ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 <  1:1592449/101‑1 (MQ=255)
                                      tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                >  1:3307249/1‑101 (MQ=255)
                                      tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                >  1:8311215/1‑101 (MQ=255)
                                      atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:7186881/98‑1 (MQ=2)
                                       tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:184020/99‑1 (MQ=2)
                                       gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               <  2:5156404/101‑1 (MQ=255)
                                       gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               >  2:5639638/1‑101 (MQ=255)
                                       gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               <  2:5781825/101‑1 (MQ=255)
                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 >  1:7677790/2‑101 (MQ=2)
                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGGGGATCGAAac                                                                 >  2:2982106/2‑101 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                >  2:5418955/1‑100 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                <  1:5556110/101‑2 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                             <  1:7824828/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATGGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                              <  2:648237/101‑4 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                              >  1:7389201/1‑98 (MQ=2)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                          <  2:8781499/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                          >  2:79879/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                          <  1:4052469/101‑1 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                            >  2:4191838/1‑96 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                           <  1:7004577/101‑7 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTgt                                                        >  2:3157882/1‑100 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                        >  2:3283867/1‑101 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                          >  1:4967330/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                          >  1:8569130/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                          >  2:2698435/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                          <  2:5301059/101‑8 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                      >  2:720983/1‑94 (MQ=37)
                                                 ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaacgac                                                      <  1:8791791/101‑8 (MQ=37)
                                                  cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                       <  1:3693557/101‑11 (MQ=0)
                                                          ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.TGCTCCCCCCGCGGGGGCGTCGATCGAAACACGGATTGCATGGCGCCGAgg                                            >  2:1599192/1‑101 (MQ=255)
                                                            cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                          <  1:5849216/101‑1 (MQ=255)
                                                            cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                          <  1:4460719/101‑1 (MQ=255)
                                                              tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                        <  1:5397863/101‑1 (MQ=255)
                                                              tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                        <  1:5164897/101‑1 (MQ=255)
                                                              tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                        <  2:6752850/101‑1 (MQ=255)
                                                              tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                        >  1:414131/1‑101 (MQ=255)
                                                               ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                       >  2:4482443/1‑101 (MQ=255)
                                                                   cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                   <  2:9048746/101‑1 (MQ=255)
                                                                   cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                   >  1:3112023/1‑101 (MQ=255)
                                                                   cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                   >  1:6277393/1‑101 (MQ=255)
                                                                    gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                  <  2:3895339/101‑1 (MQ=255)
                                                                    gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                  >  2:1476317/1‑101 (MQ=255)
                                                                    gAAAC..GGCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                  <  2:1110265/101‑1 (MQ=255)
                                                                     aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                 <  2:6924661/101‑1 (MQ=255)
                                                                      aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                >  1:2610157/1‑101 (MQ=255)
                                                                       aC..GACTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCATTGcg                               >  2:823431/1‑100 (MQ=255)
                                                                               ccGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                         <  1:4584629/101‑1 (MQ=255)
                                                                                cGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                        <  2:8890638/101‑1 (MQ=255)
                                                                                cGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                        >  2:8573432/1‑101 (MQ=255)
                                                                                 gCTCTGCTTCAAG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                       <  2:765171/101‑1 (MQ=255)
                                                                                  ctctGCTTCACG.GCTGCGTCAACCTT..GTCCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                       <  1:8941057/101‑1 (MQ=255)
                                                                                   tctGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                     >  2:7315799/1‑101 (MQ=255)
                                                                                    ctGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                    <  2:8498379/101‑1 (MQ=255)
                                                                                    ctGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                    <  1:571481/101‑1 (MQ=255)
                                                                                     tGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                   <  2:6014629/101‑1 (MQ=255)
                                                                                     tGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                   >  1:632138/1‑101 (MQ=255)
                                                                                       cTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc                 >  1:6011364/1‑101 (MQ=255)
                                                                                        ttCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                >  2:6329115/1‑101 (MQ=255)
                                                                                         tCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg               <  1:7587921/101‑1 (MQ=255)
                                                                                            cG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg            >  2:2142165/1‑101 (MQ=255)
                                                                                             g.gCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc           <  1:1055972/101‑1 (MQ=40)
                                                                                               gCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg          >  2:220581/1‑101 (MQ=40)
                                                                                                     tCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:7575545/101‑1 (MQ=33)
                                                                                                     tCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:2845532/101‑1 (MQ=33)
                                                                                                      cAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGagat   >  1:3824700/1‑98 (MQ=17)
                                                                                                      cAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  2:4913378/101‑1 (MQ=32)
                                                                                                      cAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg   >  2:637929/1‑101 (MQ=32)
                                                                                                      cAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:2846586/101‑1 (MQ=32)
                                                                                                       aaCCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa  >  1:6194166/1‑101 (MQ=31)
                                                                                                       |                                                                                                       
TCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GT.CGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1434309‑1434509

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG