Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,184 C→A 95.1% intergenic (‑112/+113) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1840C→A95.1% 40.4 ‑1.1 ‑0.9456intergenic (‑112/+113)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (220/208):  ref base (11/16):  total (231/225)
Fisher's exact test strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.22e-01

Only 100 of 468 total aligned reads displayed.

AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696085‑1696285
                                                                                                     |                                                                                                      
aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.cc                                                                                                    >  1:3989713/1‑101 (MQ=255)
 aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.ccc                                                                                                   <  2:2536666/101‑1 (MQ=255)
  gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.cccc                                                                                                  <  1:4470687/101‑2 (MQ=37)
    gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg                                                                                                >  2:759173/1‑101 (MQ=37)
    gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg                                                                                                >  1:3249673/1‑101 (MQ=37)
    gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg                                                                                                >  1:8575659/1‑101 (MQ=37)
     ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCggg                                                                                                <  2:1735253/101‑1 (MQ=37)
     ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCggg                                                                                                >  1:1660251/1‑101 (MQ=255)
      cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa                                                                                               >  1:4820394/1‑101 (MQ=255)
      cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa                                                                                               <  2:5858495/101‑1 (MQ=255)
      cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa                                                                                               <  2:2348854/101‑1 (MQ=255)
        gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatg                                                                                             >  2:241690/1‑99 (MQ=255)
         aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgt                                                                                            <  1:1333073/101‑4 (MQ=255)
          aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgtt                                                                                           <  2:2847999/101‑5 (MQ=255)
          aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgtt                                                                                           >  1:7000019/1‑97 (MQ=25)
           ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgttt                                                                                          <  1:1850359/101‑6 (MQ=25)
            tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg                                                                                         >  2:3765442/1‑101 (MQ=255)
            tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg                                                                                         >  1:3407393/1‑101 (MQ=255)
            tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg                                                                                         >  1:60860/1‑101 (MQ=255)
            tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.ACCGGGATGTTTg                                                                                         >  2:8135342/1‑101 (MQ=255)
            tGAACGTCTGTTTAATGTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg                                                                                         <  2:1987588/101‑1 (MQ=255)
              aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTggg                                                                                       >  2:4817701/1‑101 (MQ=255)
              aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTggg                                                                                       >  1:5445828/1‑101 (MQ=255)
                cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGcc                                                                                     <  1:5125584/101‑1 (MQ=255)
                  tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaa                                                                                   <  2:4175530/101‑1 (MQ=255)
                   cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                  <  1:23557/101‑2 (MQ=255)
                   cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                  <  2:4973319/101‑2 (MQ=255)
                   cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                  <  1:3154232/101‑2 (MQ=255)
                   cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                  >  1:3570014/1‑100 (MQ=21)
                    tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                 <  1:4869644/101‑3 (MQ=19)
                      tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                               >  1:5919440/1‑97 (MQ=19)
                      tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                               <  2:6389652/101‑5 (MQ=255)
                      tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                               >  1:937686/1‑97 (MQ=19)
                       ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                              >  1:7179833/1‑96 (MQ=255)
                        tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                             <  2:1178228/101‑7 (MQ=255)
                         aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                            <  1:5807632/101‑8 (MQ=255)
                          aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                         <  1:6180845/101‑1 (MQ=16)
                          aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                         >  2:4623506/1‑101 (MQ=255)
                           tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                        <  2:528911/101‑2 (MQ=255)
                           tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                        >  1:2925836/1‑100 (MQ=255)
                             tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GCTT.CCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                      <  1:8962488/101‑4 (MQ=16)
                              cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGAGCCAA..GGTGAAacacct                                                                     <  2:5626890/101‑5 (MQ=255)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGTGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  1:6253168/101‑6 (MQ=255)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    >  1:9120559/1‑96 (MQ=12)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    >  2:8271786/1‑96 (MQ=12)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  1:2775950/101‑6 (MQ=255)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    >  1:776384/1‑96 (MQ=12)
                               gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  2:4512623/101‑6 (MQ=255)
                                 aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  1:8313772/1‑94 (MQ=255)
                                 aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  <  1:2547182/101‑8 (MQ=255)
                                 aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  2:7690978/1‑94 (MQ=255)
                                 aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGCGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  <  1:4673592/101‑8 (MQ=255)
                                    cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                               <  2:6073741/101‑11 (MQ=255)
                                    cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                               >  1:3883826/1‑91 (MQ=255)
                                       gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                          <  2:3249574/101‑1 (MQ=255)
                                        cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         <  1:1117682/101‑1 (MQ=255)
                                        cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         <  1:7956894/101‑1 (MQ=255)
                                          cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                       >  2:1406863/1‑101 (MQ=255)
                                           gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                      >  1:367493/1‑101 (MQ=9)
                                           gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                      <  1:4395264/101‑1 (MQ=255)
                                            aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GCTGAAA.CACCTT.GCTTTTTCTTGgc                                                     <  2:4371030/101‑1 (MQ=9)
                                               tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                  >  2:8637723/1‑101 (MQ=255)
                                               tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                  >  1:5535828/1‑101 (MQ=255)
                                                ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 >  2:5131253/1‑101 (MQ=255)
                                                ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 <  1:6827683/101‑1 (MQ=255)
                                                 tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                <  2:92221/101‑1 (MQ=255)
                                                 tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                >  2:1655709/1‑101 (MQ=9)
                                                  gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  1:4313734/101‑1 (MQ=9)
                                                  gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               >  1:6702561/1‑101 (MQ=255)
                                                  gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               >  1:5716164/1‑101 (MQ=255)
                                                  gCAAGGAGGGGAGAGGGGC..CCTGCCCCGCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  2:7048738/101‑1 (MQ=9)
                                                   cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              >  2:6671757/1‑101 (MQ=255)
                                                   cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              <  2:4739872/101‑1 (MQ=255)
                                                     aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            >  1:97208/1‑101 (MQ=255)
                                                      ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                           >  2:3435811/1‑101 (MQ=255)
                                                        aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         >  2:7878682/1‑101 (MQ=12)
                                                        aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  1:1576521/101‑1 (MQ=9)
                                                        aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  2:6914665/101‑1 (MQ=9)
                                                         ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                        >  1:7512077/1‑101 (MQ=12)
                                                          gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:8804757/101‑1 (MQ=255)
                                                          gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       >  2:4047540/1‑101 (MQ=9)
                                                               agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                    <  1:4623641/91‑1 (MQ=255)
                                                               agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                    <  2:5330525/91‑1 (MQ=255)
                                                                   gggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                >  2:3527922/7‑101 (MQ=16)
                                                                     gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                              <  1:8457017/97‑1 (MQ=255)
                                                                      cgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                             <  1:4088655/98‑1 (MQ=16)
                                                                        ctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                           <  1:1264021/100‑1 (MQ=255)
                                                                          gCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         >  2:221261/1‑101 (MQ=255)
                                                                            cccTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                       <  2:6181029/101‑1 (MQ=16)
                                                                            cccTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                       >  1:7420814/1‑101 (MQ=255)
                                                                               tcctGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                    >  2:5395481/4‑101 (MQ=255)
                                                                                  tGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                 >  2:1844309/1‑101 (MQ=255)
                                                                                    gCCTTTCGCC.CAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa               <  2:8089587/101‑1 (MQ=19)
                                                                                          cG.G.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc         <  2:9014228/101‑1 (MQ=19)
                                                                                            ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg        >  1:2186895/4‑101 (MQ=255)
                                                                                               cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc     >  2:2695705/1‑101 (MQ=255)
                                                                                                aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTGGGCGCAAATCCGCCTCTGGCCCGAAGGCCAAGCCGCGCCGAAGGCGCGGccc    <  1:2019150/101‑1 (MQ=255)
                                                                                                aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc    <  2:4540326/101‑1 (MQ=25)
                                                                                                aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGAGCAAGCCGCGCCGAAGGCCAGGccc    >  2:7234286/1‑101 (MQ=19)
                                                                                                  ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGATGGCGCGGccccc  <  1:8121503/101‑1 (MQ=25)
                                                                                                     |                                                                                                      
AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696085‑1696285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG