Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,317 G→A 1.9% intergenic (‑113/‑192) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3170G→A 1.9% 11.7 ‑1.1 ‑0.2469intergenic (‑113/‑192)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (216/243):  total (222/247)
Fisher's exact test strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.02e-01

Only 100 of 493 total aligned reads displayed.

ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416
                                                                                                      |                                                                                                            
 tgggcgtTCCTGAGATGCACAGATACCGCAGCTTGGGCAACCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                             <  2:2908010/96‑1 (MQ=38)
   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATGTTTGAGGCa                                                                                                           >  2:8636348/1‑101 (MQ=255)
   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                           <  1:3955613/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                     <  2:604612/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                    <  1:3539289/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                    >  1:307811/1‑101 (MQ=255)
           tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                <  2:2596144/101‑1 (MQ=255)
             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                              >  1:2171462/1‑101 (MQ=255)
              gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                             <  1:3471470/101‑1 (MQ=255)
               aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                          >  1:4668825/1‑101 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       >  1:7984106/1‑101 (MQ=255)
                   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                      <  2:5080921/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                     <  2:8343677/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                     <  2:7925949/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                    <  2:1571314/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                    >  2:9144745/1‑101 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                    <  2:748514/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGATCTG.CCCACGTCCCGTGCCGGGACT.CCACAGGGATATTTGAGGCAAGATGAA...GGGG..GGCgtta                                                                                    >  1:4228236/1‑100 (MQ=38)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                   <  2:7812568/101‑1 (MQ=255)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                  <  1:93810/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                <  2:6423256/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                >  2:7358618/1‑101 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                               <  1:5339678/101‑1 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                               <  1:2734237/101‑1 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCCGGATATTTGAGGCAAGATGAA...GGGG..GGGGTTCATcct                                                                               >  1:1751418/1‑99 (MQ=25)
                           cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGT..GGCTTTCATCTTg                                                                              <  1:8193465/101‑1 (MQ=255)
                            gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                             <  2:4864751/101‑1 (MQ=255)
                            gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                             <  1:3784828/101‑1 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                           >  1:2085417/1‑101 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                           >  1:3333887/1‑101 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                          >  1:5883854/1‑101 (MQ=255)
                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                         >  1:2605045/1‑101 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                       <  2:4654239/101‑1 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATATTGCCGaaaa                                                                       <  1:7775653/101‑1 (MQ=255)
                                       aCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATcc                                                                  >  1:7564228/1‑101 (MQ=255)
                                       aCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATcc                                                                  >  1:1798221/1‑101 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                 <  1:1004950/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                 <  1:512212/101‑1 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                               >  1:6665859/1‑101 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                               >  2:9101089/1‑101 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                               >  1:143857/1‑101 (MQ=255)
                                                aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                         >  2:1420596/1‑101 (MQ=255)
                                                aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                         >  2:1184804/1‑101 (MQ=255)
                                                 agaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                        >  2:370838/1‑101 (MQ=255)
                                                  gaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                       >  1:5820317/1‑101 (MQ=255)
                                                   aagTGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                     <  2:5672356/101‑1 (MQ=255)
                                                   aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                     <  2:3604025/101‑1 (MQ=255)
                                                      ggggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                  <  2:3410576/101‑1 (MQ=255)
                                                      ggggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                  <  2:3123724/101‑1 (MQ=255)
                                                       gggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                 <  1:449453/101‑1 (MQ=255)
                                                        ggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GAGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TAgcgc                                                >  2:4211706/1‑101 (MQ=37)
                                                        ggCTCTGCCCC.CGTCCCGCGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                <  1:2973/101‑1 (MQ=255)
                                                            ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                          <  2:706044/97‑1 (MQ=255)
                                                             tgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                         <  2:2452754/98‑1 (MQ=255)
                                                              gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                        <  1:8949544/99‑1 (MQ=255)
                                                              gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                        <  1:7663264/99‑1 (MQ=255)
                                                               cccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                       >  2:6629917/2‑101 (MQ=255)
                                                                 cc.cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                     <  1:1521022/101‑1 (MQ=255)
                                                                  c.cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                    >  1:7366470/1‑101 (MQ=255)
                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                 >  2:5483648/1‑101 (MQ=255)
                                                                       cccgtGCCGGGGCT.CTCCCGGGGTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGGCGAAAAGATACCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                <  1:1606239/101‑1 (MQ=38)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                              <  2:8502363/101‑1 (MQ=255)
                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                  >  1:1126642/8‑101 (MQ=21)
                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                             <  2:5204198/101‑1 (MQ=255)
                                                                          gtGACGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                             <  1:2906103/101‑1 (MQ=255)
                                                                           caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                >  1:4364353/6‑101 (MQ=18)
                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                          >  2:5365/1‑101 (MQ=255)
                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                        >  1:4960159/1‑101 (MQ=255)
                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                        <  2:8191464/101‑1 (MQ=255)
                                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                       <  1:6049399/101‑1 (MQ=255)
                                                                                ggACT.CCCCCGGGATATTTGAGGCAGGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                      <  2:1779782/101‑1 (MQ=255)
                                                                                 gACT.CCCCCGGGATATTTGACGCAAGATGAN...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccc                     <  1:7209702/101‑2 (MQ=255)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                   <  1:2422173/101‑4 (MQ=255)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                   <  2:1484146/101‑4 (MQ=255)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                   <  1:6918400/101‑4 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  1:2004924/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  1:771031/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  2:2771868/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  2:2020464/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  2:1281900/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  <  2:108225/101‑5 (MQ=255)
                                                                                      cccccGGTATATTTGCGGCAAGATGAA...GTGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCt                     <  2:8937294/101‑1 (MQ=2)
                                                                                       ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgc                 <  1:8837916/101‑1 (MQ=255)
                                                                                       ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgc                 >  2:6221132/1‑101 (MQ=255)
                                                                                        cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTCTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGTGGGGCAGCGCCCCCCTTcgcg                >  1:6446734/1‑101 (MQ=25)
                                                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccc                 >  1:4744183/1‑97 (MQ=0)
                                                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtagat                 >  1:8676867/1‑97 (MQ=0)
                                                                                          cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..NCCAGAGGGGGGCAGCGCCCCCCTTCgcggg              >  1:655564/1‑101 (MQ=255)
                                                                                             gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcgg           >  1:5603106/1‑101 (MQ=255)
                                                                                               tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc         >  2:5563506/1‑101 (MQ=255)
                                                                                               tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc         <  2:3930156/101‑1 (MQ=255)
                                                                                                 tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTAGCGGGCGGCcgc       >  1:6244415/1‑101 (MQ=255)
                                                                                                  ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg      >  2:5853825/1‑101 (MQ=255)
                                                                                                  ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg      <  2:7677818/101‑1 (MQ=255)
                                                                                                   tGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGa     <  1:7902126/101‑1 (MQ=255)
                                                                                                    gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc    <  2:2203705/101‑1 (MQ=255)
                                                                                                    gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc    >  1:8079337/1‑101 (MQ=255)
                                                                                                     aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg   <  2:2065188/101‑1 (MQ=255)
                                                                                                      ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc  >  2:4059790/1‑101 (MQ=255)
                                                                                                      ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc  <  1:1319276/101‑1 (MQ=255)
                                                                                                      |                                                                                                            
ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG