Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,462,450 G→C 100% M103M (GTG→CTG)  RCAP_rcc02286 → divalent ion symporter family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,462,4500G→C100.0% 1424.2 385M103M (GTG→CTG) RCAP_rcc02286divalent ion symporter family
Reads supporting (aligned to +/- strand):  new base (164/221):  ref base (0/0):  total (164/221)

Only 100 of 403 total aligned reads displayed.

TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAC  >  NC_014034/2462352‑2462547
                                                                                                   |                                                                                                 
tGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTg                                                                                                 <  2:1605862/101‑1 (MQ=255)
tGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTg                                                                                                 <  2:2378219/101‑1 (MQ=255)
 gACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGc                                                                                                <  1:7714790/101‑1 (MQ=255)
    gcgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCttt                                                                                             <  2:7399070/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTc                                                                                            <  1:8919954/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTc                                                                                            <  2:3699548/101‑1 (MQ=255)
      ggACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCg                                                                                           <  1:1510515/101‑1 (MQ=255)
      ggACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCg                                                                                           <  1:2846181/101‑1 (MQ=255)
       gACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGa                                                                                          <  2:8746403/101‑1 (MQ=255)
        aCCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAg                                                                                         >  1:8588388/1‑101 (MQ=255)
        aCCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAg                                                                                         >  1:7574657/1‑101 (MQ=255)
        aCCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGCCGGCGGGCTGCTTTCGAg                                                                                         <  2:1426027/101‑1 (MQ=255)
         ccGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGc                                                                                        >  1:4753068/1‑101 (MQ=255)
             ggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGtga                                                                                    <  2:4882904/101‑1 (MQ=255)
              gCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGtgat                                                                                   <  2:7183805/101‑1 (MQ=255)
               cGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGtgatg                                                                                  <  2:2895637/101‑1 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGCGCGtgatga                                                                                 >  1:2162456/1‑101 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGtgatga                                                                                 <  2:349228/101‑1 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGtgatga                                                                                 >  1:8820551/1‑101 (MQ=255)
                  tCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAAc                                                                               <  1:5489452/101‑1 (MQ=255)
                   cAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACa                                                                              <  1:2439556/101‑1 (MQ=255)
                   cAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACa                                                                              <  1:7004307/101‑1 (MQ=255)
                     gcgcgcTGGCGCTGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAAt                                                                            <  2:5843597/101‑1 (MQ=255)
                     gcgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAAt                                                                            <  1:5219159/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  1:802444/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  1:4457511/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          >  2:4019685/1‑101 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGTATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                         <  2:8423894/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                         <  2:5715247/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                         >  2:6003445/1‑101 (MQ=255)
                          cTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAc                                                                       >  2:7836197/1‑101 (MQ=255)
                           tGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAcc                                                                      <  2:4691136/101‑1 (MQ=255)
                           tGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAcc                                                                      <  2:3765078/101‑1 (MQ=255)
                           tGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAcc                                                                      <  2:5411631/101‑1 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCGGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                     <  2:7547510/101‑1 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                     >  2:66802/1‑101 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                     <  1:5070160/101‑1 (MQ=255)
                             gcgcGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcgc                                                                    <  1:6189024/101‑1 (MQ=255)
                                 ggCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTg                                                                <  2:2684907/101‑1 (MQ=255)
                                 ggCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTg                                                                <  1:7419303/101‑1 (MQ=255)
                                    ttCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgcg                                                             <  1:8254749/101‑1 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                           >  2:4312367/1‑101 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                           <  2:8873212/101‑1 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                           >  1:4837600/1‑101 (MQ=255)
                                       tGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtt                                                          <  2:3613296/101‑1 (MQ=255)
                                       tGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtt                                                          >  2:1508429/1‑101 (MQ=255)
                                        gTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                         >  2:7759352/1‑101 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                        <  2:3279775/101‑1 (MQ=255)
                                          cGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTg                                                       <  1:6704290/101‑1 (MQ=255)
                                           gAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGGCACCGCGCTGGCGCTTTTGa                                                      >  1:5617123/1‑101 (MQ=255)
                                            aaCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGAt                                                     >  2:6836376/1‑101 (MQ=255)
                                            aaCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGAt                                                     >  2:6564703/1‑101 (MQ=255)
                                              cccGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGc                                                   >  1:6338862/1‑101 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                                 <  1:6011770/101‑1 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                                 <  2:2411102/101‑1 (MQ=255)
                                                 gccgcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCg                                                <  2:6717982/99‑1 (MQ=255)
                                                    gcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCg                                             <  2:2284087/101‑1 (MQ=255)
                                                    gcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCg                                             >  2:5044121/1‑101 (MQ=255)
                                                      tGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGAc                                           >  1:5399417/1‑101 (MQ=255)
                                                      tGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGAc                                           >  2:1899711/1‑101 (MQ=255)
                                                      tGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGAc                                           <  1:6477741/101‑1 (MQ=255)
                                                      tGCCGGTCAGCCTGCATGGGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGAc                                           <  1:9133400/101‑1 (MQ=255)
                                                       gCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACa                                          <  1:5336853/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        <  2:5509524/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        <  1:2618454/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        <  2:8150805/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        >  1:8768284/1‑101 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        <  2:275630/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGAGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTCTGATGCCCGTCGACATc                                        >  2:5235851/1‑101 (MQ=255)
                                                          ggTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCg                                       >  1:273279/1‑101 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                     <  2:6113443/101‑1 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                     <  1:3331427/101‑1 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                     <  1:131497/101‑1 (MQ=255)
                                                              aGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCAc                                   <  1:7870314/101‑1 (MQ=255)
                                                              aGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCAc                                   <  1:2345266/101‑1 (MQ=255)
                                                                ccTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACgg                                 >  2:7959267/1‑101 (MQ=255)
                                                                ccTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACgg                                 <  2:3035298/101‑1 (MQ=255)
                                                                 cTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgc                                <  1:3595736/101‑1 (MQ=255)
                                                                 cTGCATGTGGCGAGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgc                                >  2:8499304/1‑101 (MQ=255)
                                                                  tGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcg                               >  2:3948315/1‑101 (MQ=255)
                                                                  tGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcg                               <  1:7150507/101‑1 (MQ=255)
                                                                      tgtgGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCa                           >  2:6366355/1‑101 (MQ=255)
                                                                      tgtgGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCa                           >  1:1939954/1‑101 (MQ=255)
                                                                       gtgGCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCaa                          >  1:1110891/1‑101 (MQ=255)
                                                                         ggCGCGGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAgg                        >  1:3894444/1‑101 (MQ=255)
                                                                           cgcgGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGcc                      <  1:5837458/101‑1 (MQ=255)
                                                                            gcgGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGccc                     <  1:29886/101‑1 (MQ=255)
                                                                             cgGCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCg                    <  1:8540408/101‑1 (MQ=255)
                                                                              ggCTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCgg                   >  1:315889/1‑101 (MQ=255)
                                                                                cTGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcg                 >  1:1007420/1‑101 (MQ=255)
                                                                                 tGGGGGT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc                >  1:1818770/1‑101 (MQ=255)
                                                                                  gggggTAGGCCGGCGGGCTGCTTTCGAGCCTGATGAACAATGTCACCGCGCTGGCGATTTTGATGCCCGTCGACTTAGCCACGGCGCGCAAGGCCCGgcgc                >  2:9063927/1‑101 (MQ=255)
                                                                                   ggggT.GGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgcgc              <  1:7535644/101‑1 (MQ=255)
                                                                                     ggt.ggtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCgg            <  2:8224987/101‑1 (MQ=255)
                                                                                       t.ggtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGcc          >  2:1112582/1‑101 (MQ=255)
                                                                                              gcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTg    <  1:4312491/101‑1 (MQ=255)
                                                                                               cggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGa   >  1:4118626/1‑101 (MQ=255)
                                                                                                gggcTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAc  <  2:7056784/101‑1 (MQ=255)
                                                                                                gggcTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAc  <  2:8588003/101‑1 (MQ=255)
                                                                                                gggcTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAc  >  1:4629150/1‑101 (MQ=255)
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TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGT.GGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAC  >  NC_014034/2462352‑2462547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG