Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 3.6% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 3.6% 16.1 0.0 ‑0.1326intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (147/167):  total (153/173)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.40e-01

Only 100 of 352 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                    |                                                                                                           
ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                             <  1:935108/101‑1 (MQ=21)
 cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                            <  2:5902061/101‑1 (MQ=21)
  gcggcgGGGGCGCGGCACGACAGGCCGGGTAAGGGGGGGGCGCTGCGCCCCCTCCCGAAGCGGCCTTCCCCCGGGGAAGTTGCAGGACGATCAAACCGGag                                                                                                           >  2:162524/1‑100 (MQ=16)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGAGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                         <  1:4318653/101‑1 (MQ=21)
      cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                       <  2:3529329/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                      <  2:1045837/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                      <  2:2294198/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                      <  2:4257301/101‑1 (MQ=21)
        ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                     <  2:92579/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                    <  1:5943665/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                    <  1:3228848/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                    <  1:3715740/101‑1 (MQ=21)
           gcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                                  <  1:1821005/101‑1 (MQ=21)
           gcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                                  <  2:2861940/101‑1 (MQ=21)
            cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                 <  1:7733596/101‑1 (MQ=21)
            cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                 <  1:2578386/101‑1 (MQ=21)
              gggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                               <  2:5377022/100‑1 (MQ=21)
              cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                               <  2:4844033/101‑1 (MQ=21)
              cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                               <  2:6857187/101‑1 (MQ=21)
                 cACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                            >  1:6997411/1‑101 (MQ=21)
                  aCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGccc                                                                                           >  2:1895580/1‑101 (MQ=18)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          >  1:8030789/1‑101 (MQ=21)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          >  1:1210863/1‑101 (MQ=21)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          <  1:6124724/101‑1 (MQ=21)
                     aCAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                        <  1:8498056/101‑1 (MQ=21)
                      cAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                       >  2:7452255/1‑101 (MQ=21)
                      cAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTACCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                       <  2:5231288/101‑1 (MQ=21)
                       aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAat                                                                                      >  2:6214733/1‑101 (MQ=18)
                       aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                      >  2:3357068/1‑101 (MQ=21)
                        ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                     <  2:4679719/101‑1 (MQ=21)
                         gCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                    >  1:864384/1‑101 (MQ=18)
                         gCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                    <  2:2140873/101‑1 (MQ=21)
                         gCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                    <  2:2527562/101‑1 (MQ=21)
                          ccGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                   <  1:52771/101‑1 (MQ=21)
                           cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                  <  2:812952/101‑1 (MQ=21)
                           cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCCAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                  >  1:1916425/1‑101 (MQ=18)
                            gAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGATGCCTTTCATCTTGCCCGAAAAATccc                                                                                 >  1:2055529/1‑101 (MQ=18)
                             aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                <  1:6236828/101‑1 (MQ=18)
                              gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                               >  2:7827681/6‑101 (MQ=255)
                              gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCGTTCATCTTGCCCGAAATATCCCgg                                                                               >  2:5025270/6‑101 (MQ=18)
                               taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                              <  1:1707389/97‑1 (MQ=255)
                               taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                              >  2:3951065/5‑101 (MQ=255)
                                aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                             >  2:3051073/4‑101 (MQ=255)
                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                           <  2:6346311/100‑1 (MQ=255)
                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCTTTCATCTTGCCCGAAATATCACggcggg                                                                           >  1:8301384/2‑101 (MQ=18)
                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCACGGGCTATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                           >  1:4038957/2‑101 (MQ=18)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                        <  1:1052772/101‑1 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGa                                                                        <  1:8155471/101‑1 (MQ=255)
                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaag                                                                      >  2:1114671/1‑100 (MQ=255)
                                        ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                    <  2:3220547/101‑1 (MQ=255)
                                         gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                   <  2:2595400/101‑1 (MQ=255)
                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                  <  1:3525941/101‑1 (MQ=255)
                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                  <  2:5992274/101‑1 (MQ=255)
                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                  >  1:734792/1‑101 (MQ=255)
                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                  <  2:7245104/101‑1 (MQ=255)
                                           gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                               <  1:3322414/101‑1 (MQ=255)
                                           gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..g                                                               >  2:700788/1‑101 (MQ=255)
                                            cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                             >  1:3848141/1‑101 (MQ=255)
                                              gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                           <  1:6370809/101‑3 (MQ=255)
                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                         >  1:6655669/1‑97 (MQ=255)
                                                  cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                       >  2:1972654/5‑95 (MQ=18)
                                                    cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGCATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                     <  1:8955443/99‑9 (MQ=21)
                                                             gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCGTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagg                                           >  1:5092912/1‑99 (MQ=18)
                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGA.GGGGGGGcgcgcc                                         >  2:5435998/1‑96 (MQ=18)
                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.c                                     >  1:714694/1‑101 (MQ=18)
                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.c                                     >  2:7126503/1‑101 (MQ=18)
                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.cc                                    >  1:489283/1‑101 (MQ=18)
                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.cc                                    >  2:8319079/1‑101 (MQ=18)
                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.c.ccc                                   <  2:882595/101‑1 (MQ=17)
                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.ccc                                   >  2:759813/1‑101 (MQ=18)
                                                                     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.ccccct                                <  1:3132562/101‑3 (MQ=17)
                                                                       cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.c.ccccctgc                              <  2:7704352/101‑5 (MQ=17)
                                                                         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGccc                             >  2:4434598/1‑101 (MQ=18)
                                                                          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.C.CCCCTGcccc                            >  2:2789263/1‑101 (MQ=0)
                                                                          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGcccc                            >  2:6501150/1‑101 (MQ=18)
                                                                             atatTTGCGGCAAGATGAAGGGTCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.C.CCCCTGCCCcgcc                         <  2:8953323/101‑3 (MQ=2)
                                                                             atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCcggc                         >  2:2661722/1‑101 (MQ=17)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCcggcg                        >  2:4504676/1‑101 (MQ=18)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCcggcg                        >  1:6499480/1‑101 (MQ=18)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                       >  1:9097314/1‑101 (MQ=17)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                       >  1:4170280/1‑101 (MQ=18)
                                                                                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTc                     <  1:7429955/101‑1 (MQ=17)
                                                                                  tGCAGCAAGATGAAACCGGATGCTTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                    <  1:4666843/101‑1 (MQ=17)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc               >  1:132532/1‑101 (MQ=18)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc               >  1:2412558/1‑101 (MQ=18)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGG.AAGCGCC.CCCCTGCCCCGCCGGACCGTgcc               >  2:3689875/1‑99 (MQ=17)
                                                                                        aaGATGAAACCGGATGCCTTTCATGTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg              >  2:4385111/1‑101 (MQ=17)
                                                                                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCTC.C.CCCCTGCCCCGGCGGTACGTGgccgcggc         >  2:1494372/1‑99 (MQ=14)
                                                                                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        <  2:1258087/101‑1 (MQ=17)
                                                                                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        >  2:3606184/1‑101 (MQ=18)
                                                                                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCCC.C.CCCCTGCCCCGCCGGCCGGTGGGGGCGCCt        >  1:1127422/1‑101 (MQ=12)
                                                                                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CGCCCCTGCCCCGCCGGTCCGCGGGGGCCccc        >  1:290759/1‑100 (MQ=9)
                                                                                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCACCGC.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt       <  2:5557655/101‑1 (MQ=18)
                                                                                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      <  1:7982313/101‑1 (MQ=17)
                                                                                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      <  2:3848032/101‑1 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  2:6788597/101‑1 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    >  1:5640879/1‑101 (MQ=18)
                                                                                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg   >  2:3784068/1‑101 (MQ=18)
                                                                                                    gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CGAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg  >  1:7691488/1‑101 (MQ=18)
                                                                                                    gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcgg  >  1:992681/1‑101 (MQ=17)
                                                                                                    |                                                                                                           
CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG