Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,273 T→C 100% T48T (ACA→ACG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,2730T→C100.0% 1449.1 389T48T (ACA→ACGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (183/206):  ref base (0/0):  total (183/206)

Only 100 of 405 total aligned reads displayed.

GCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959175‑2959370
                                                                                                  |                                                                                                 
gCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAgcgt                                                                                                 >  1:5096351/1‑101 (MQ=255)
  aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                                                                                               >  1:8154645/1‑101 (MQ=255)
  aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                                                                                               >  2:1311043/1‑101 (MQ=255)
    tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGGGCGTCGcc                                                                                             >  1:1642658/1‑101 (MQ=255)
    tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc                                                                                             <  1:4994036/101‑1 (MQ=255)
    tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc                                                                                             <  2:2772796/101‑1 (MQ=255)
    tgatgaATTCCAGCGCCTCAACGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc                                                                                             <  1:5609306/101‑1 (MQ=255)
      atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt                                                                                           >  2:896181/1‑101 (MQ=255)
      atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt                                                                                           <  1:9085037/101‑1 (MQ=255)
       tgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTg                                                                                          >  1:598174/1‑101 (MQ=255)
        gaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTgg                                                                                         >  2:2789968/1‑101 (MQ=255)
         aaTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGa                                                                                        >  1:5271472/1‑101 (MQ=255)
            tCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGacac                                                                                     >  2:7534383/1‑101 (MQ=255)
            tCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGacac                                                                                     <  1:8485945/101‑1 (MQ=255)
             ccAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcc                                                                                    <  1:8896347/101‑1 (MQ=255)
             ccAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTAGCCTTGGACAcc                                                                                    >  1:9114444/1‑101 (MQ=255)
              cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc                                                                                   >  1:2233598/1‑101 (MQ=255)
               aGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcccc                                                                                  >  2:2625212/1‑101 (MQ=255)
                gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  1:5504796/101‑1 (MQ=255)
                gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  1:3227274/101‑1 (MQ=255)
                  gcctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGCGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGc                                                                               >  1:8222172/4‑101 (MQ=255)
                   cctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGGAATAGCTGTAGAGCGTCGCCTTGGACGCCCCCGcc                                                                              >  1:2993459/3‑101 (MQ=255)
                   cctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGcc                                                                              >  1:4205575/3‑101 (MQ=255)
                    ctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCt                                                                             <  1:7058025/100‑1 (MQ=255)
                     tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  1:8748939/101‑1 (MQ=255)
                     tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            >  2:8173364/1‑101 (MQ=255)
                     tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  2:4790841/101‑1 (MQ=255)
                     tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  1:2525821/101‑1 (MQ=255)
                     ccATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  2:3635377/100‑1 (MQ=255)
                     ccATCGCCCTGCCGCTGGCATTCGCCCGTCGCGATCGCTATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTc                                                                            <  2:6459843/100‑1 (MQ=255)
                       aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  2:4356460/1‑101 (MQ=255)
                       aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  1:1001285/1‑101 (MQ=255)
                       aTCGGCCTGGCGCTGGCATTCGCCCGACGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  1:6354215/1‑101 (MQ=255)
                              tGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGAt                                                                   >  1:518247/1‑101 (MQ=255)
                                gcgcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGt                                                                 <  2:4715371/101‑1 (MQ=255)
                                    tGGCATTCCCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATc                                                             <  2:598164/101‑1 (MQ=255)
                                     ggCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCg                                                            <  2:2459043/101‑1 (MQ=255)
                                        aTTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAcc                                                         >  1:7519380/1‑101 (MQ=255)
                                         ttCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCg                                                        <  2:3790415/101‑1 (MQ=255)
                                         ttCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCg                                                        <  1:3514208/101‑1 (MQ=255)
                                         ttCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCg                                                        <  1:1754083/101‑1 (MQ=255)
                                            gCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAg                                                     <  1:4503805/101‑1 (MQ=255)
                                             cccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTAATCGACCGAAgc                                                    >  2:4601664/1‑101 (MQ=255)
                                               cGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcgc                                                  <  2:1089961/101‑1 (MQ=255)
                                                gTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGcc                                                 >  2:2011233/1‑101 (MQ=255)
                                                  cgcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               >  2:2183086/1‑101 (MQ=255)
                                                  cccgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               <  2:798287/99‑1 (MQ=255)
                                                   gcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              >  1:7445780/1‑101 (MQ=255)
                                                   gcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              <  1:1390322/101‑1 (MQ=255)
                                                     gATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGa                                            <  1:269476/101‑1 (MQ=255)
                                                     gATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGa                                            >  1:539702/1‑101 (MQ=255)
                                                      aTCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGaa                                           <  2:4057650/101‑1 (MQ=255)
                                                       tCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAc                                          >  1:4451529/1‑101 (MQ=255)
                                                        cGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAcc                                         <  1:1311075/101‑1 (MQ=255)
                                                         gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  1:3965074/101‑1 (MQ=255)
                                                         gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  2:7048219/101‑1 (MQ=255)
                                                           cATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGt                                      <  2:5274235/101‑1 (MQ=255)
                                                           cATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGt                                      >  1:7801798/1‑101 (MQ=255)
                                                            aTGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTc                                     >  2:8731009/1‑101 (MQ=255)
                                                            aTGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTc                                     <  1:8581969/101‑1 (MQ=255)
                                                            aTGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTc                                     <  2:329135/101‑1 (MQ=255)
                                                             tGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcg                                    <  2:180516/101‑1 (MQ=255)
                                                             tGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCCTcg                                    >  2:2595233/1‑101 (MQ=255)
                                                              gAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   >  1:4071824/1‑101 (MQ=255)
                                                                aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 >  2:6541533/1‑101 (MQ=255)
                                                                aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 >  2:8530833/1‑101 (MQ=255)
                                                                aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 >  2:6097224/1‑101 (MQ=255)
                                                                  agcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAg                               <  1:3694643/101‑1 (MQ=255)
                                                                  agcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAg                               <  1:4356586/101‑1 (MQ=255)
                                                                    cagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaa                             >  1:207200/1‑101 (MQ=255)
                                                                    cagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaa                             <  2:7485231/101‑1 (MQ=255)
                                                                    cagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaa                             >  2:3102634/1‑101 (MQ=255)
                                                                    cacccGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaa                             <  2:5596142/98‑1 (MQ=255)
                                                                     agcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaaa                            <  2:847487/101‑1 (MQ=255)
                                                                     agcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaaa                            <  1:6541793/101‑1 (MQ=255)
                                                                       ccGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAaca                          <  1:6191842/101‑1 (MQ=255)
                                                                          cTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACtt                       <  1:3416915/101‑1 (MQ=255)
                                                                           ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      <  2:9059160/101‑1 (MQ=255)
                                                                           ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      <  1:2360082/101‑1 (MQ=255)
                                                                            tGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTc                     >  2:5095803/1‑101 (MQ=255)
                                                                             gTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTcc                    <  1:8024615/101‑1 (MQ=255)
                                                                              tCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcg                   >  2:4463403/1‑101 (MQ=255)
                                                                              tCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcg                   <  2:5777194/101‑1 (MQ=255)
                                                                               ccGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcgc                  >  2:1576094/1‑101 (MQ=255)
                                                                               ccGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcgc                  >  2:7686169/1‑101 (MQ=255)
                                                                                    aaaTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCgcgcgcg             >  1:3835959/1‑100 (MQ=255)
                                                                                    aaaTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGcc             <  2:6918842/101‑1 (MQ=255)
                                                                                    aaaTAGCGGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGcc             <  2:1374984/101‑1 (MQ=255)
                                                                                      aTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGt           >  2:3427368/1‑101 (MQ=255)
                                                                                      aTAGCTGTAGAGCGTCGCCTCGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGt           <  1:7205918/101‑1 (MQ=255)
                                                                                        aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         <  2:3038332/101‑1 (MQ=255)
                                                                                        aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         <  2:15336/101‑1 (MQ=255)
                                                                                         gCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCaa        >  2:7277972/1‑101 (MQ=255)
                                                                                          cTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAg       <  2:5484659/101‑1 (MQ=255)
                                                                                           tGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCTTCGCGCAGAAACACTTTCCGCGCGCCGTCAAgg      <  1:4783847/101‑1 (MQ=255)
                                                                                            gTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGa     >  1:5855045/1‑101 (MQ=255)
                                                                                              agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAcc   >  1:2837778/1‑101 (MQ=255)
                                                                                              agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAcc   >  1:6059804/1‑101 (MQ=255)
                                                                                               gagCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCt  <  1:665742/101‑1 (MQ=255)
                                                                                               gagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCt  <  2:4970287/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959175‑2959370

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG