Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,020 A→C 10.8% intergenic (+66/+110) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0200A→C10.8% 138.0 ‑1.8 ‑0.2551intergenic (+66/+110)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (32/27):  ref base (215/271):  total (251/300)
Fisher's exact test strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.22e-01

Only 100 of 577 total aligned reads displayed.

CGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGG  >  NC_014034/3003920‑3004120
                                                                                                     |                                                                                                    
cGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGa                                                                                                      <  1:6953648/101‑1 (MQ=255)
  atgatCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGAcc                                                                                                    >  2:2730100/1‑101 (MQ=255)
  atgatCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGAcc                                                                                                    >  2:6873474/1‑101 (MQ=255)
    gatcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCaa                                                                                                  >  1:7396316/1‑101 (MQ=255)
       cgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGCGCTATGTAGACCAACGt                                                                                               >  2:5305158/1‑101 (MQ=38)
          tcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaa                                                                                            <  2:2214371/101‑1 (MQ=255)
           cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaaa                                                                                           >  2:160936/1‑101 (MQ=255)
           cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaaa                                                                                           <  1:2342347/101‑1 (MQ=255)
            tGGAGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGGTATTTGGACCAAGGTGAAAg                                                                                          >  1:7237903/1‑101 (MQ=255)
             ggTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGc                                                                                         >  2:850329/1‑101 (MQ=255)
             ggTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGc                                                                                         <  1:5586592/101‑1 (MQ=255)
             ggTACGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGc                                                                                         >  1:8793929/1‑101 (MQ=255)
             ggTACGAGCGGGCGAGATCTGAGGGAAAGGC.GGGGGGCGCAGCCCCCCTCTTGGCCTGAGAGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGc                                                                                         <  2:2628178/101‑1 (MQ=37)
                 gccgcgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAt                                                                                     <  2:9182461/99‑1 (MQ=255)
                   cgcgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATcc                                                                                   <  2:8910064/101‑1 (MQ=255)
                   cgcgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATcc                                                                                   <  2:5863656/101‑1 (MQ=255)
                    gcgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGAAGATccc                                                                                  <  1:6113933/101‑1 (MQ=255)
                     cgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCg                                                                                 <  2:2783707/101‑1 (MQ=255)
                     cgGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCg                                                                                 >  1:7067794/1‑101 (MQ=255)
                        gCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGtt                                                                              >  2:8800488/1‑101 (MQ=255)
                         ggagaTCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  2:1271933/100‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGCGGGGGGGCCCTGCCCCCCTCTTGGCCTACCGGCAAATTCCCCCCCCGGGAAATTTGGCCCACGGTGAAGGCAACTCCCGGtt                                                                              >  2:3796866/1‑101 (MQ=19)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  1:3315442/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  2:8145948/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  2:1052723/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  2:400978/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  1:5730604/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             <  2:6525799/101‑1 (MQ=255)
                         cGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                                                             >  1:3039368/1‑101 (MQ=255)
                          gagaTCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                                                            <  2:4582004/101‑1 (MQ=255)
                          gagaTCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                                                            <  2:5083131/101‑1 (MQ=255)
                              tCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCtt                                                                        <  1:1974266/101‑1 (MQ=255)
                               cTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCttt                                                                       >  2:6405548/1‑101 (MQ=255)
                               cTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCttt                                                                       <  2:425563/101‑1 (MQ=255)
                               cTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCttt                                                                       <  1:3518893/101‑1 (MQ=255)
                                tGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                                                      <  1:5036649/101‑1 (MQ=255)
                                tGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                                                      <  1:2916740/101‑1 (MQ=255)
                                tGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                                                      <  2:6684455/101‑1 (MQ=255)
                                 gAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCa                                                                     <  1:8536736/101‑1 (MQ=255)
                                   gggCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCAcc                                                                   <  2:7197304/101‑1 (MQ=255)
                                      cAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTg                                                                <  1:8185252/101‑1 (MQ=255)
                                       aaGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTgg                                                               <  1:2165314/101‑1 (MQ=255)
                                           c.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTACACCTTGGCGCa                                                           >  1:9101620/1‑101 (MQ=255)
                                               ggggCCCTGCCCCCCTCTTGGCCTTACGGCCAATTCTCCCCCCGGGATATTTGGACCAAGGTAAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                        <  1:6468882/101‑1 (MQ=37)
                                                gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATACCGGTTTGCCTTTCACCTTGGCGCAAata                                                       <  1:8932460/101‑1 (MQ=255)
                                                 ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACACCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                      <  1:5893039/101‑1 (MQ=255)
                                                  gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                     <  1:6801499/101‑1 (MQ=255)
                                                    gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                   >  2:7386600/1‑101 (MQ=255)
                                                    gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                   <  2:3333873/101‑1 (MQ=255)
                                                     cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                  <  1:1063229/101‑1 (MQ=255)
                                                      tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                 <  1:2756517/101‑1 (MQ=255)
                                                       gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                >  2:4349313/1‑101 (MQ=255)
                                                        ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                               <  1:7558854/101‑1 (MQ=255)
                                                         cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                              <  1:3827533/101‑1 (MQ=255)
                                                          ccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                             >  1:4459725/1‑101 (MQ=255)
                                                             ctctTGGTCTGACGGCCAAATCACCCCCCGGGATATTTGGACCAAGGTGAAATCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                          <  1:5943719/101‑1 (MQ=255)
                                                              tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                         >  1:5420441/1‑101 (MQ=255)
                                                               ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                        >  1:2510985/1‑101 (MQ=255)
                                                               ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                        <  1:1814609/101‑1 (MQ=255)
                                                                 tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                      >  2:5526598/1‑101 (MQ=255)
                                                                 tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                      <  1:8632364/101‑1 (MQ=255)
                                                                 tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                      >  1:7852565/1‑101 (MQ=255)
                                                                      tGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                 <  2:7838192/101‑1 (MQ=255)
                                                                      tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                 >  2:7601777/1‑101 (MQ=255)
                                                                      tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                 <  2:8699035/101‑1 (MQ=255)
                                                                      tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                 >  2:628269/1‑101 (MQ=255)
                                                                             cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                          >  2:1187647/1‑101 (MQ=255)
                                                                             cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCACGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                          >  1:7145626/1‑101 (MQ=255)
                                                                              aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                         <  2:4461813/101‑1 (MQ=255)
                                                                               aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                        <  1:2671473/101‑1 (MQ=255)
                                                                               aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                        <  1:4639324/101‑1 (MQ=255)
                                                                                 tcgccccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                      <  1:8090651/98‑1 (MQ=255)
                                                                                  cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                     <  2:1936245/101‑1 (MQ=255)
                                                                                    ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                   >  2:2880086/1‑101 (MQ=18)
                                                                                     cccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCaccgcc                  >  2:6456643/1‑96 (MQ=21)
                                                                                     cccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                  <  1:4234450/101‑1 (MQ=255)
                                                                                     cccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                  <  2:8299375/101‑1 (MQ=255)
                                                                                      ccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                 <  1:632330/101‑1 (MQ=255)
                                                                                        ccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGCCGGGGGGCAGAgccccc               >  1:5278632/1‑101 (MQ=255)
                                                                                        ccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc               <  1:8363446/101‑1 (MQ=255)
                                                                                          gggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc             <  2:3910189/101‑2 (MQ=17)
                                                                                           ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct            >  1:1652929/1‑99 (MQ=18)
                                                                                           ggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg            <  1:320702/101‑1 (MQ=255)
                                                                                           ggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg            <  1:1460919/101‑1 (MQ=255)
                                                                                           ggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATAACCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAgcccccctg            >  2:2520192/1‑101 (MQ=255)
                                                                                            gATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc           <  1:4017896/101‑4 (MQ=17)
                                                                                            gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCTGAgcccccctgc           <  1:6573023/101‑1 (MQ=255)
                                                                                             atatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc          >  2:6138005/1‑101 (MQ=255)
                                                                                              tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt         <  1:482196/101‑6 (MQ=17)
                                                                                              tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc         >  2:6792763/1‑101 (MQ=255)
                                                                                               atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc        <  2:8017715/101‑7 (MQ=17)
                                                                                               atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc        >  2:3537998/1‑101 (MQ=255)
                                                                                               atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc        <  2:3039269/101‑1 (MQ=255)
                                                                                               atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc        <  2:1415990/101‑1 (MQ=255)
                                                                                                tttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc       >  2:4754631/1‑101 (MQ=255)
                                                                                                 ttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc      <  2:316894/101‑1 (MQ=255)
                                                                                                  tGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct     <  2:5700460/101‑1 (MQ=255)
                                                                                                  tGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct     <  2:2323914/101‑1 (MQ=255)
                                                                                                   ggACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcacccctg    >  1:9074883/1‑101 (MQ=255)
                                                                                                     aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg  >  2:1751043/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGC.GGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGG  >  NC_014034/3003920‑3004120

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG