Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 590,814 C→G 100% Q56E (CAA→GAA)  murI → glutamate racemase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034590,8140C→G100.0% 1691.1 455Q56E (CAA→GAA) murIglutamate racemase
Reads supporting (aligned to +/- strand):  new base (207/248):  ref base (0/0):  total (207/248)

Only 100 of 466 total aligned reads displayed.

TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911
                                                                                                  |                                                                                                 
tGCCCGAGGTGGCCTTTGTCTATTATGGCGCCAATGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 <  1:4245652/101‑1 (MQ=255)
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 <  1:2530664/101‑1 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  1:3614128/1‑101 (MQ=255)
  cccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACg                                                                                               >  1:3434735/1‑101 (MQ=255)
  cccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACg                                                                                               >  2:6389959/1‑101 (MQ=255)
    cGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGc                                                                                             <  2:8465310/101‑1 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGcc                                                                                            >  2:2182200/1‑100 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            <  2:1886173/101‑1 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           <  1:1938808/101‑1 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           <  2:6666802/101‑1 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       <  1:4582743/101‑1 (MQ=255)
          ggCCTTTGTCTATTAGGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       <  1:960622/101‑1 (MQ=255)
           gCCTTTGTCTGTTATGGCGACAAGGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGCCCTGCCGCGGCGTCGAACGGCTGTGGGa                                                                                      <  2:6047308/101‑1 (MQ=255)
           gCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGTGGCGTCGAACGGCTGTGGGa                                                                                      <  1:8224856/101‑1 (MQ=255)
            ccTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGAc                                                                                     <  2:4577149/101‑1 (MQ=255)
              tttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGa                                                                                   >  2:366969/1‑101 (MQ=255)
              tttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGa                                                                                   >  1:7538746/1‑101 (MQ=255)
              tttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGa                                                                                   >  1:1053246/1‑101 (MQ=255)
                tGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgg                                                                                 >  1:8458005/1‑101 (MQ=255)
                   cTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCt                                                                              >  1:6610911/1‑101 (MQ=255)
                    tattatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTg                                                                             <  2:1337133/101‑1 (MQ=255)
                     attatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGGCGAACGGCTGTGGGACGAGGGCTGc                                                                            >  2:7971932/1‑101 (MQ=255)
                      ttatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCg                                                                           >  1:3243279/1‑101 (MQ=255)
                       tatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGa                                                                          >  2:3020420/1‑101 (MQ=255)
                       tatGGCGACAATGCCCATGCGCCCTACGCCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGa                                                                          >  1:6513882/1‑101 (MQ=255)
                       tatAGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGa                                                                          >  1:7700764/1‑101 (MQ=255)
                        atGGCGACAATGCCCATGCGCCCTACGGCGGGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGAt                                                                         <  1:6832365/101‑1 (MQ=255)
                        atGGCGACAATGCCCATGCGCCCTACGGCGGGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGAt                                                                         <  1:1168421/101‑1 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        <  1:5687955/101‑1 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        >  1:1420163/1‑101 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        <  1:1609284/101‑1 (MQ=255)
                          ggCGACAATGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCt                                                                       <  1:4920788/101‑1 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTg                                                                   <  1:9077708/101‑1 (MQ=255)
                              aCAATGACCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTgggg                                                                   >  2:271745/1‑99 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  2:7801743/1‑101 (MQ=255)
                                aaggCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 <  2:509100/98‑1 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 >  2:4441176/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGGGAt                                                                 >  2:621018/1‑101 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               <  1:8921359/101‑1 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               <  2:349847/101‑1 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTCCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               <  2:6514551/101‑1 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          >  2:2590973/1‑101 (MQ=255)
                                        tGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCt                                                         >  1:8406477/1‑101 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        >  1:8699300/1‑101 (MQ=255)
                                          cgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGc                                                       <  1:5424520/101‑1 (MQ=255)
                                           gcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      <  2:7700438/101‑1 (MQ=255)
                                           gcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      <  2:3557198/101‑1 (MQ=255)
                                             ccTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    <  2:5486191/101‑1 (MQ=255)
                                             ccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    <  2:1066553/101‑1 (MQ=255)
                                              ctccGGCGTGCGCCCCCCCGACGACATCTACAACCTGCCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  1:1105459/98‑1 (MQ=255)
                                               tACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  1:6973131/101‑1 (MQ=255)
                                                    cGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCt                                             <  1:3355645/101‑1 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  1:8567378/1‑101 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  1:481023/1‑101 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  2:810649/1‑101 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  2:7284041/1‑101 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  1:13536/1‑101 (MQ=255)
                                                      ggcgcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTcc                                           <  2:2448424/100‑1 (MQ=255)
                                                         gcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        >  1:5160478/1‑101 (MQ=255)
                                                          cACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgccg                                       <  2:5443060/101‑1 (MQ=255)
                                                           ccccccGACTACATCTACAACCGGGCCTGCNGCGGCGTCGAACGGCTGTGGTACGAGGGCTGCGATCTGGGGATTCTGGCCTGCAACACGGCCTCcgccgc                                      <  1:2748057/100‑1 (MQ=255)
                                                           aCCCCCGACGACCTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGGGATTCTGGCCTGCAACACGGCCTCcgccgc                                      >  1:8133144/1‑101 (MQ=255)
                                                              cccGGCGACATCTACATCCTGGCCTGCCGCTGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggc                                   <  2:3850998/101‑1 (MQ=255)
                                                              cccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggc                                   <  1:8849464/101‑1 (MQ=255)
                                                                 gacgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGAGCt                                >  2:1630268/1‑101 (MQ=255)
                                                                  acgacATCTACAACCTGGCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  2:4688559/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGCCCTGCCGCGGCGTCGAACGGCTGTGAGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  2:9083798/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  2:6814386/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  1:3020519/101‑1 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              >  2:6991469/1‑101 (MQ=255)
                                                                    gacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  2:3243180/101‑1 (MQ=255)
                                                                     acATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaaa                            <  2:7543468/101‑1 (MQ=255)
                                                                      cATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaaaa                           >  2:8977275/1‑101 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCGCCGCCGCGGCGGTGAAAAg                          >  2:240966/1‑101 (MQ=255)
                                                                        tCTACAACCTGACCTGCCGCCGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAACAgg                         <  1:7228516/101‑1 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        >  1:4517049/1‑101 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        >  2:5702175/1‑101 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  1:3560772/1‑101 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  1:6726667/1‑101 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAGAGGGagg                      >  2:2923749/1‑99 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATg                      >  1:291016/1‑101 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATg                      <  1:2345009/101‑1 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     <  2:4760361/101‑1 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     <  1:1777521/101‑1 (MQ=255)
                                                                            cAACCGGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     <  2:5387874/101‑1 (MQ=255)
                                                                               cctgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAgg                  <  1:6145393/101‑1 (MQ=255)
                                                                                cgggcctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGa                 <  1:3976690/97‑1 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               <  1:5820674/101‑1 (MQ=255)
                                                                                    cctcCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  1:7339005/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            <  2:5939501/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            <  2:1526362/101‑1 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           <  2:6855672/101‑1 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           >  1:3766719/1‑101 (MQ=255)
                                                                                        ccGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGa         <  2:3951646/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:8288108/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:8789062/101‑1 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    <  1:7864531/101‑1 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  1:6045148/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  >  2:1184651/1‑101 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  <  2:6344797/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG