Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 625,791 G→C 100% R533R (CGG→CGC anfA → nitrogen fixation regulatory protein AnfA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034625,7910G→C100.0% 1681.6 461R533R (CGG→CGCanfAnitrogen fixation regulatory protein AnfA
Reads supporting (aligned to +/- strand):  new base (216/245):  ref base (0/0):  total (216/245)

Only 100 of 479 total aligned reads displayed.

ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888
                                                                                                  |                                                                                                   
 cgccccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                  <  2:2942970/98‑1 (MQ=255)
 cgacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTAt                                                                                                  <  1:5687634/101‑1 (MQ=255)
   acCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcg                                                                                                >  1:4730970/1‑101 (MQ=255)
     ccAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcgcg                                                                                              >  1:1181387/1‑101 (MQ=255)
       aGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCt                                                                                            >  2:7791538/1‑101 (MQ=255)
        gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                           >  1:5212621/1‑101 (MQ=255)
        gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                           <  2:821307/101‑1 (MQ=255)
         ggCAATATCGGCCAGGCGGCGGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTc                                                                                          <  1:7204199/101‑1 (MQ=255)
          gCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCg                                                                                         <  1:4371602/101‑1 (MQ=255)
            aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                       >  2:1726042/1‑101 (MQ=255)
            aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCCCACCGCTATCCCGCTTCGGc                                                                                       >  2:4467252/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                      >  1:1115313/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                      >  1:6315516/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGcc                                                                                      >  2:3541123/1‑101 (MQ=255)
             atatCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTAGGcc                                                                                      >  2:5822910/1‑101 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTGGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                  <  2:8724845/101‑1 (MQ=255)
                 cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                  <  1:929663/101‑1 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                 >  2:6307322/1‑101 (MQ=255)
                  ggCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATc                                                                                 <  2:2510435/101‑1 (MQ=255)
                   gCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCg                                                                                <  1:1805558/101‑1 (MQ=255)
                    ccAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGa                                                                               >  1:6481903/1‑101 (MQ=255)
                    ccAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGa                                                                               <  2:1872763/101‑1 (MQ=255)
                      cggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACg                                                                             <  2:4219534/100‑1 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                         >  1:4529825/1‑101 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                         <  1:5031282/101‑1 (MQ=255)
                          ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                         >  1:8494475/1‑101 (MQ=255)
                             cGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATg                                                                      >  2:5238074/1‑101 (MQ=255)
                              gAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGa                                                                     >  2:2360692/1‑101 (MQ=255)
                              gAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGa                                                                     >  2:3846943/1‑101 (MQ=255)
                               atcTTTTGCAGGTCTCGCGCCGTGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAc                                                                    <  2:4100952/99‑1 (MQ=255)
                                gCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGAcc                                                                   <  1:4937680/101‑1 (MQ=255)
                                 cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                  <  1:4119220/101‑1 (MQ=255)
                                 cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                  >  2:4354090/1‑101 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGGTCGACGGCCAATGACCGa                                                                 >  2:4995142/1‑101 (MQ=255)
                                  ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                                 >  1:7982485/1‑101 (MQ=255)
                                   tttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGaa                                                                <  1:6948547/101‑1 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                              <  1:5506357/101‑1 (MQ=255)
                                     tGCAGGTCTCGCGCCGGGTGCGGGCGCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                              <  1:4243651/101‑1 (MQ=255)
                                      gCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAc                                                             >  2:840777/1‑101 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                            >  2:8578834/1‑101 (MQ=255)
                                       cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                            >  1:34114/1‑101 (MQ=255)
                                           tctcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcc                                                        >  2:8926829/1‑101 (MQ=255)
                                            ctcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGccc                                                       <  1:6214562/101‑1 (MQ=255)
                                             tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                      <  1:7502752/101‑1 (MQ=255)
                                               gcgcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGc                                                    <  2:7916317/101‑1 (MQ=255)
                                                 gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                  <  2:300881/101‑1 (MQ=255)
                                                 gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                  >  2:4728202/1‑101 (MQ=255)
                                                 gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                  <  2:1115253/101‑1 (MQ=255)
                                                  ccGTGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                 <  2:7563711/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                 <  2:9160400/101‑1 (MQ=255)
                                                  ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                                 <  1:7077390/101‑1 (MQ=255)
                                                   cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                                >  2:6456464/1‑101 (MQ=255)
                                                   cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                                >  1:7809030/1‑101 (MQ=255)
                                                     ggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggc                                              >  2:3676061/1‑101 (MQ=255)
                                                       tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                            >  1:5419339/1‑101 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                           <  1:1416953/101‑1 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                           >  1:1738081/1‑101 (MQ=255)
                                                        gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                           >  2:5103145/1‑101 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                         >  1:3190495/1‑101 (MQ=255)
                                                          tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                         >  1:8180057/1‑101 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                       <  1:2743122/101‑1 (MQ=255)
                                                            ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                       <  2:5389005/101‑1 (MQ=255)
                                                             gCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttt                                      <  1:1001745/101‑1 (MQ=255)
                                                              gcTGCGTCTGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGGCCCGCTTCGGCGGGGCtttt                                     <  1:5965433/100‑1 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                    >  2:1401542/1‑101 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttttt                                    >  2:4032532/1‑101 (MQ=255)
                                                               cTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCgtttt                                    >  2:2733928/1‑101 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCt                                  <  2:3335691/101‑1 (MQ=255)
                                                                 gCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCt                                  <  2:6663722/101‑1 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                                 >  1:2341963/1‑101 (MQ=255)
                                                                  cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                                 >  2:5508050/1‑101 (MQ=255)
                                                                   ggATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCttt                                >  1:8265366/1‑101 (MQ=255)
                                                                      tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                             <  1:6711508/101‑1 (MQ=255)
                                                                      tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                             >  2:6788498/1‑101 (MQ=255)
                                                                      tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                             <  1:7603098/101‑1 (MQ=255)
                                                                      tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                             <  2:8800434/101‑1 (MQ=255)
                                                                       ggggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCgg                            >  1:3643121/1‑101 (MQ=255)
                                                                       ggggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCgg                            <  1:3541230/101‑1 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.c                          <  2:2196111/101‑1 (MQ=255)
                                                                        gggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.c                          <  2:6198135/101‑1 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.Gccc                    <  2:3135494/101‑1 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.Gccc                    <  1:5778912/101‑1 (MQ=255)
                                                                             gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.Gccc                    >  1:392438/1‑101 (MQ=255)
                                                                                cGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGGGG.CAGGCCCGt                  >  2:7333825/1‑101 (MQ=255)
                                                                                cGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GGCCggt                 >  1:2435882/1‑99 (MQ=255)
                                                                                cGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGtt                 <  1:4076095/101‑1 (MQ=255)
                                                                                 ggCATCCACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTc                <  1:2949305/101‑1 (MQ=255)
                                                                                  gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCg               <  1:1292132/101‑1 (MQ=255)
                                                                                  gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCg               <  2:7163954/101‑1 (MQ=255)
                                                                                  gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGGTCg               >  1:3849199/1‑101 (MQ=255)
                                                                                   cATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCgg              <  1:8734398/101‑1 (MQ=255)
                                                                                    aTCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACTGAATCCTGGTCCCGCTTCGGCGCGGCCTTTTCTTCTCGGCCG.GCCCGTTcta              >  2:2321006/1‑99 (MQ=255)
                                                                                      cGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCCTTTGGGAt           >  2:8134793/1‑101 (MQ=255)
                                                                                         cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCGGTTCGGAATTcc        <  1:9022911/101‑1 (MQ=255)
                                                                                         cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTcc        <  2:1587787/101‑1 (MQ=255)
                                                                                            gCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGtt     <  1:1572720/101‑1 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGttt    <  1:3058805/101‑1 (MQ=255)
                                                                                             cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGttt    <  2:5601277/101‑1 (MQ=255)
                                                                                              aCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAACTCCCTTTa   >  2:1235218/1‑101 (MQ=255)
                                                                                               ccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGttttc  >  2:5966386/1‑99 (MQ=255)
                                                                                                  |                                                                                                   
ACGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGG.CA.GCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625693‑625888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG