Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,645	G→C	100%	intergenic (‑99/+21)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,645	0	G→C	100.0%	199.3	52	intergenic (‑99/+21)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (46/6):  ref base (0/0):  total (46/6)

CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.CGA.C.G.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774595‑774728                                                       |                                                                                     cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg                                     >  1:1711243/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg                                     >  2:3479180/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg                                     >  1:5666101/11‑101 (MQ=255)   caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                   >  2:2409717/9‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTc                                  >  1:436300/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                  >  2:8919022/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                  >  1:7872427/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                  >  1:3574470/8‑101 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTa                             >  2:3086055/3‑101 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGATCCAGTa                             >  2:6513074/3‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGGCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCCCTTCGCCCCCGACCAGTCGGCCACGTTCCAGCAt                            >  2:3287223/2‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAt                            >  2:1642341/2‑101 (MQ=255)           gcgcCCCCCCGCCCGGGCCGGCCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAtt                           <  2:3369043/101‑1 (MQ=255)                      cccgggtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAg                >  2:6331017/11‑101 (MQ=255)                        cgggtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc              >  1:22934/9‑101 (MQ=255)                          ggtcggaCCGGCCCTGCCGGGC.CGACC.C.TACGGTCCCTTATCCTGCGATCAGTTCGCCCGCGACCAGTCTGCCACGTTCCAGTATTCGCCGTCCCCGCCGt            >  2:8732924/7‑101 (MQ=255)                          ggtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  2:6175517/7‑101 (MQ=255)                          ggtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  1:2446695/7‑101 (MQ=255)                           gtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:7503056/6‑101 (MQ=255)                           gtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:3372372/6‑101 (MQ=255)                           gtcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  2:2657396/6‑101 (MQ=255)                            tcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAgatcgga          >  2:408449/5‑95 (MQ=255)                            tcggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc          >  1:3966135/5‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:2046870/4‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  2:6782371/4‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  2:5738700/4‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:4891747/4‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCCGA.CAC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  2:6243087/4‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  1:5855913/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  1:799673/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  2:4081238/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  1:2349184/3‑101 (MQ=255)                               gaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  1:8972134/2‑101 (MQ=255)                               gaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  2:4880622/2‑101 (MQ=255)                               gaCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  2:7356948/2‑101 (MQ=255)                                cccGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      <  2:8313875/100‑1 (MQ=255)                                cccGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      <  2:1587134/100‑1 (MQ=255)                                cccGCCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      <  2:3607051/100‑1 (MQ=255)                                aCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      >  2:8219904/1‑101 (MQ=255)                                aCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      >  2:7692121/1‑101 (MQ=255)                                aCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      >  1:7620002/1‑101 (MQ=255)                                aCCGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      >  1:4877803/1‑101 (MQ=255)                                 ccGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     >  1:7384154/1‑101 (MQ=255)                                 ccGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     >  1:6019343/1‑101 (MQ=255)                                  cGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCg    >  2:4965528/1‑101 (MQ=255)                                  cGGCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCg    >  1:8519921/1‑101 (MQ=255)                                   ggCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  1:3213128/1‑101 (MQ=255)                                   ggCCCTGCC.GGTCCGA.C.CCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  1:5312606/1‑101 (MQ=255)                                    gCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  2:3213029/101‑1 (MQ=255)                                    gCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  >  2:5494104/1‑101 (MQ=255)                                    gCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  2:8119331/101‑1 (MQ=255)                                    gCCCTGCC.GGTCCGACC.C.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  >  2:374246/1‑101 (MQ=255)                                                       |                                                                                     CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.CGA.C.G.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774595‑774728 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG