Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,011 Δ1 bp 13.1% intergenic (+823/‑108) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0110C→.13.1% 134.1 0.0 0.0327intergenic (+823/‑108)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/23):  ref base (131/152):  total (152/175)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCG  >  NC_014034/1395911‑1396111
                                                                                                    |                                                                                                          
 cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                                                                                                           >  1:2059999/1‑101 (MQ=255)
    gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                                                                        >  1:4342230/1‑101 (MQ=255)
     ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                                       <  1:1277307/101‑1 (MQ=255)
         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   >  1:905913/1‑101 (MQ=255)
         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                                                                                                   <  1:2248563/101‑1 (MQ=255)
          ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..a                                                                                                >  1:2434660/1‑101 (MQ=255)
           gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.c                                                                                              <  2:3752537/101‑1 (MQ=255)
            aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.Cg                                                                                             >  2:4445474/1‑101 (MQ=255)
            aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.Cg                                                                                             <  1:3450828/101‑1 (MQ=255)
              cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGcc                                                                                           <  1:4301024/101‑1 (MQ=255)
              cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGcc                                                                                           <  2:2485090/101‑1 (MQ=255)
              cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGcc                                                                                           <  2:2056968/101‑1 (MQ=255)
               gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCt                                                                                          <  2:2886886/101‑1 (MQ=255)
               gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCt                                                                                          <  1:2598521/101‑1 (MQ=255)
                aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTg                                                                                         >  1:4184517/1‑101 (MQ=255)
                 aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGc                                                                                        >  1:5784933/1‑101 (MQ=255)
                  aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGc.c                                                                                      <  2:175315/101‑1 (MQ=255)
                  aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGc.c                                                                                      <  2:1721336/101‑1 (MQ=255)
                   cGAAGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.cg                                                                                     <  1:1938485/101‑1 (MQ=255)
                     aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.cgc..g                                                                                 <  1:2963119/101‑1 (MQ=255)
                      tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..Gt                                                                                >  2:3471322/1‑101 (MQ=255)
                       gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTc                                                                               >  1:870105/1‑101 (MQ=255)
                        cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCg                                                                              <  2:2095655/101‑1 (MQ=255)
                          ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCt                                                                            >  2:5294532/1‑101 (MQ=255)
                           tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTc                                                                           <  2:3710421/101‑1 (MQ=255)
                           tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTc                                                                           <  2:4420718/101‑1 (MQ=255)
                              tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTcccc                                                                        <  1:2896471/101‑1 (MQ=255)
                                gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTcccccc                                                                      <  2:3079456/101‑1 (MQ=255)
                                 ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCcg                                                                     <  1:2696813/101‑1 (MQ=255)
                                 ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCcg                                                                     >  2:3854736/1‑101 (MQ=255)
                                   tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                   >  1:2082973/1‑101 (MQ=255)
                                   tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                   <  2:5213403/101‑1 (MQ=255)
                                        cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                              >  1:3645473/1‑101 (MQ=255)
                                        cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                              <  2:3327705/101‑1 (MQ=255)
                                          ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCgg                                                            >  2:1405589/1‑100 (MQ=255)
                                          ggCAGTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                            <  2:3000441/101‑1 (MQ=255)
                                          ggCAGGGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                            <  2:4181830/101‑1 (MQ=255)
                                          atctgTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..C.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                            <  1:1444162/97‑1 (MQ=255)
                                            cAGAGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                          >  2:162945/1‑101 (MQ=255)
                                             aggggccTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  1:1007181/95‑1 (MQ=38)
                                             aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  2:5041334/101‑1 (MQ=255)
                                              tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  1:338147/8‑101 (MQ=2)
                                              gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:1247863/101‑1 (MQ=255)
                                               tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:5530928/101‑1 (MQ=255)
                                               tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  1:4875965/1‑101 (MQ=255)
                                                 gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  2:108028/97‑1 (MQ=9)
                                                  aCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  1:3196842/101‑1 (MQ=255)
                                                   gcggTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:644739/99‑1 (MQ=9)
                                                    tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTTGATCGGCGCCTGCCTC..A.CGCAAGC.ACC..GTCGCTCCCCCCGCCGGTGCGAGGAACGAAAc                                                  >  2:5706081/1‑101 (MQ=21)
                                                    tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCNGGGGCGTGGATCGAAAc                                                  >  2:2564574/1‑101 (MQ=255)
                                                    tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  >  1:4107320/1‑101 (MQ=255)
                                                    cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  2:1542836/2‑101 (MQ=2)
                                                     ggTGGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 <  1:4093882/101‑1 (MQ=255)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  <  2:4889750/101‑1 (MQ=9)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  >  1:1595654/1‑101 (MQ=9)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                               >  2:720038/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                               >  2:4259927/1‑98 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                               >  2:544813/1‑98 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                              >  1:5436433/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                             >  1:3070142/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                             <  2:338103/101‑6 (MQ=2)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTTGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                            >  2:1336826/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                            >  1:640657/1‑101 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                            >  1:5663087/1‑95 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                            <  2:4964659/101‑7 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                            >  1:4916508/1‑95 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTCGCACTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                           >  1:5165462/1‑101 (MQ=255)
                                                           cccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                           <  1:4348552/100‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                           >  1:1508549/1‑94 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC....GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                           >  1:5368863/1‑94 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                          <  2:2741324/101‑1 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTATCGCGCGC.TGC...TCGCTCCCCCCGCGGGGGCGTGGTTCGAAActtgatgac                                          >  1:4807478/1‑93 (MQ=2)
                                                               cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                                       <  2:5357209/101‑1 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                    >  1:4715810/1‑101 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAtct                                   <  2:2243709/101‑3 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCT.CCCCCGCGGGGGCGTGGGTCGAAACAGGCGCATCTGTTGta                                  >  2:4005173/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                  >  2:1962199/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGCGGGTCGAAACATGCGCATCTGATGtt                                  >  2:5565979/1‑100 (MQ=255)
                                                                       ggCGTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                               <  2:3645245/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                              <  2:832704/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                              <  1:250983/101‑1 (MQ=255)
                                                                          gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                            >  1:1347778/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                            >  1:3291325/1‑101 (MQ=255)
                                                                           tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                           >  1:5245079/1‑101 (MQ=255)
                                                                           tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                           <  1:3760206/101‑1 (MQ=255)
                                                                            ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                          >  1:4509022/1‑101 (MQ=255)
                                                                              aTCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                        <  1:2354471/101‑1 (MQ=255)
                                                                              aTCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                        <  2:2396051/101‑1 (MQ=255)
                                                                                cGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                      >  1:402712/1‑101 (MQ=255)
                                                                                 gAAACTCGCCCTCGATCCTCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                     <  2:482323/101‑1 (MQ=255)
                                                                                 caaaCCCGCCCTCGATCCGCGCTTGCCTC..C.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                     <  1:1075237/100‑1 (MQ=255)
                                                                                  aaaCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                    >  2:2336446/1‑101 (MQ=255)
                                                                                   aaCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                   >  1:3885628/1‑101 (MQ=255)
                                                                                    aCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                  >  1:1160518/1‑101 (MQ=255)
                                                                                    aCTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                  <  1:1637352/101‑1 (MQ=255)
                                                                                         cccTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt             <  1:4448939/101‑1 (MQ=255)
                                                                                          ccTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc            <  2:5634478/101‑1 (MQ=255)
                                                                                              gATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc        >  2:3284934/1‑101 (MQ=255)
                                                                                                   gcgcTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc   <  2:5164008/101‑1 (MQ=255)
                                                                                                    cgcTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg  <  1:5117788/101‑1 (MQ=255)
                                                                                                    |                                                                                                          
GCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC..A.CGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCG  >  NC_014034/1395911‑1396111

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG