Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,555 Δ1 bp 2.0% coding (1437/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5550C→. 2.0% 9.5 ‑0.4 ‑0.2305coding (1437/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (145/153):  total (148/157)
Fisher's exact test strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.40e-01

Only 100 of 347 total aligned reads displayed.

TGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397455‑1397654
                                                                                                          |                                                                                                     
tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agc                                                                                                       <  1:5565396/101‑1 (MQ=255)
tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agc                                                                                                       >  1:4335459/1‑101 (MQ=255)
  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     <  2:3433266/101‑1 (MQ=255)
  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     >  2:25384/1‑101 (MQ=255)
  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     >  2:1663967/1‑101 (MQ=255)
  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.Agcgc                                                                                                     >  1:3710900/1‑101 (MQ=255)
       aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCgcgc                                                                                                >  2:74260/1‑101 (MQ=255)
         acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.g                                                                                             <  1:1104576/101‑1 (MQ=255)
          cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.Ga                                                                                            >  2:3625373/1‑101 (MQ=255)
            ccGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.Cg                                                                                         <  2:3767670/101‑1 (MQ=255)
             cGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.ctt                                                                                        <  1:3844756/101‑3 (MQ=255)
                 atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                                    >  1:2607650/1‑101 (MQ=255)
                   caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                                  <  2:5085233/101‑1 (MQ=255)
                       acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                              >  2:901066/1‑101 (MQ=255)
                       acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                              <  1:5364708/101‑1 (MQ=255)
                        cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                             <  2:1346350/101‑1 (MQ=255)
                        cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                             <  1:380966/101‑1 (MQ=255)
                           aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                          >  2:3552075/1‑101 (MQ=255)
                           aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                          >  2:5380486/1‑101 (MQ=255)
                              cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                       >  1:2345018/1‑101 (MQ=255)
                               gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      <  1:474413/101‑1 (MQ=255)
                               gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      >  1:796788/1‑101 (MQ=255)
                               gCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                      <  1:3957730/101‑1 (MQ=255)
                                  cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:3712684/101‑1 (MQ=255)
                                  cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:4245535/101‑1 (MQ=255)
                                   ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:1010202/91‑1 (MQ=2)
                                    gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  2:5194685/101‑1 (MQ=255)
                                     caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:1679812/101‑1 (MQ=255)
                                       tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:5621194/95‑1 (MQ=0)
                                        ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA..CTGACCCGGCAC.C..GGCCTA.TGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:1842902/3‑101 (MQ=2)
                                        aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  2:3980391/96‑1 (MQ=2)
                                        accgcgTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGATGATCAAA.TC.C..GCAATC.GGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:1553335/96‑1 (MQ=0)
                                         gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:1270437/4‑101 (MQ=2)
                                          tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  2:3320313/98‑1 (MQ=2)
                                           tggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:4795059/100‑1 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                        <  1:5595504/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                        <  1:4196028/101‑2 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                        >  2:4563723/1‑100 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggc                                                     >  1:4491237/1‑95 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                      <  2:137027/101‑2 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       <  1:3035318/101‑3 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       >  2:1495369/1‑99 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       <  1:2082591/101‑3 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                       >  2:4682133/1‑99 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                      <  1:886857/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGGTCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                     >  1:3286248/1‑99 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcg                                                    >  2:2311462/1‑95 (MQ=0)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                      <  2:3157138/101‑4 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                     >  1:1189406/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaac                                                     >  1:749210/1‑97 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                   >  1:2855559/1‑97 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCT..GT.C..GGCCGCGAACACCGATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                                   >  2:2835365/1‑97 (MQ=2)
                                                  cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                   <  2:5013856/100‑5 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                  >  1:495962/1‑96 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                   >  2:4355957/1‑95 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                  >  1:3733821/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.CGCGCCGCTCT.CA.CGTCGTTCCTCCCGCGGGTGCGTTTATCTAAActcacgaaga                                                 >  2:310365/1‑92 (MQ=21)
                                                      cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                               >  2:1545276/1‑101 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACAAC.CCGGTCATG.GC.C..CGCCGC.AGCGCCGCGCT.GA.CGCCCCTCCCCCCGCGGGGGCGGGGATCGAAACAACCGCGGTTCg                                              >  2:1872416/1‑101 (MQ=37)
                                                       ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                              >  2:4692683/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                            >  1:2243043/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                            <  1:1745408/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                           <  1:1440409/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                         <  2:1941575/101‑1 (MQ=255)
                                                                 gTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                    <  2:1714259/101‑1 (MQ=255)
                                                                  tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                   >  1:4624084/1‑101 (MQ=255)
                                                                  tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                   >  2:5476236/1‑101 (MQ=255)
                                                                  tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                   >  2:4110810/1‑101 (MQ=255)
                                                                   ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                  >  2:3849294/1‑101 (MQ=255)
                                                                   ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccccc                                  >  2:723943/1‑101 (MQ=255)
                                                                    gATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                 >  2:4969/1‑101 (MQ=255)
                                                                    gATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                 >  2:2158615/1‑101 (MQ=255)
                                                                     aTCAAAACACC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                <  2:1167460/101‑1 (MQ=255)
                                                                      tCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                               >  2:4626226/1‑101 (MQ=255)
                                                                        gAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                             >  1:2196941/1‑101 (MQ=255)
                                                                           acacc.CCGATCAGG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                          <  1:2523282/101‑1 (MQ=255)
                                                                           acaac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                          <  2:3822088/101‑1 (MQ=255)
                                                                              ac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                       >  2:2436555/1‑101 (MQ=255)
                                                                                  cGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg                    >  2:2743840/1‑101 (MQ=255)
                                                                                    aTCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                  >  2:3248869/1‑101 (MQ=255)
                                                                                    aTCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                  >  2:2368872/1‑101 (MQ=255)
                                                                                    aTCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                  <  2:2649768/101‑1 (MQ=255)
                                                                                      cATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                <  1:3074553/101‑1 (MQ=255)
                                                                                       aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcct               <  1:5195753/101‑2 (MQ=255)
                                                                                       aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:2660663/1‑101 (MQ=255)
                                                                                       aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:3611044/1‑101 (MQ=255)
                                                                                       aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:4835748/1‑101 (MQ=255)
                                                                                       aTG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc               >  1:97823/1‑101 (MQ=255)
                                                                                        tG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccc              <  1:339128/101‑1 (MQ=255)
                                                                                         g.gC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc             <  1:3554506/101‑1 (MQ=255)
                                                                                           gC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc            <  1:5083292/101‑1 (MQ=255)
                                                                                            gac..cGCCGC.AGCGCCGCGCT.GA.CGACGCACCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc            <  1:2794470/99‑1 (MQ=255)
                                                                                            c.c..cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg           <  1:243880/101‑1 (MQ=255)
                                                                                            c.c..cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg           <  1:3342108/101‑1 (MQ=255)
                                                                                                 cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg         <  1:2144085/101‑1 (MQ=255)
                                                                                                    cTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg      >  2:2912373/1‑101 (MQ=255)
                                                                                                    cTC.AGCGCCGCGCG.GA.CGTCGCCCCCCCCGCGGGGGGGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg      <  1:5780207/101‑1 (MQ=38)
                                                                                                     tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg     >  2:5792056/1‑101 (MQ=255)
                                                                                                      c.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc    <  2:4180287/101‑1 (MQ=255)
                                                                                                        aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg   >  2:868676/1‑101 (MQ=255)
                                                                                                          |                                                                                                     
TGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397455‑1397654

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG