Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,916 Δ1 bp 100% intergenic (+186/‑161) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9160G→.100.0% 664.6 151intergenic (+186/‑161)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (51/100):  ref base (0/0):  total (51/100)

Only 100 of 180 total aligned reads displayed.

GATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGC  >  NC_014034/1607818‑1608014
                                                                                                  |                                                                                                  
 aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                               <  1:843652/101‑1 (MQ=255)
 aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                               <  2:5236386/101‑1 (MQ=255)
 aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                               <  2:5677226/101‑1 (MQ=255)
 aTCGGGGCGGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                               <  1:2915394/101‑1 (MQ=255)
  tCGGGGCGGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCg                                                                                              <  1:1727497/101‑1 (MQ=255)
   cGGGGCTGACGCCCGCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCgg                                                                                             <  2:667254/101‑1 (MQ=255)
       gCTGGCGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                                         <  2:3505162/101‑1 (MQ=255)
       gCTGCCGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                                         <  2:5696119/101‑1 (MQ=255)
       gCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                                         <  1:100438/101‑1 (MQ=255)
       gCTGACCCCCCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                                         <  2:3130145/101‑1 (MQ=255)
        cTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGG.GAGACCGGGCCga                                                                                        >  2:1160923/1‑100 (MQ=255)
        cTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCga                                                                                        <  2:4905937/101‑2 (MQ=255)
        cTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCga                                                                                        <  2:3826594/101‑2 (MQ=255)
         tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGG.GCGACCGGGGCgac                                                                                       >  2:4190638/1‑99 (MQ=255)
         tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgac                                                                                       <  2:3839337/101‑3 (MQ=255)
             gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgcc                                                                                   <  2:638714/101‑7 (MQ=255)
                gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCCggccgccccc                                                                                >  2:1492607/1‑93 (MQ=255)
                gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                               <  2:1022441/101‑1 (MQ=255)
                gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.CGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                               >  2:1232304/1‑101 (MQ=255)
                gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGCCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                               <  2:5364857/101‑1 (MQ=255)
                 ccccGCCCGCATTGGGGCCGCCTCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCt                                                                              <  2:1902785/98‑1 (MQ=255)
                   ccGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGt                                                                            <  1:591804/100‑1 (MQ=255)
                    cGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                           <  2:2759604/101‑1 (MQ=255)
                     gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.ATACCGGGCC.GACCGCCGCCCGTCt                                                                          >  1:3068325/1‑101 (MQ=255)
                       ccGCATTGTGGCCGCCGCTCCCCACAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                        <  2:3268579/101‑3 (MQ=255)
                       ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                        >  2:2186432/1‑99 (MQ=255)
                        cgcttggtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                       <  2:2496030/95‑4 (MQ=255)
                         gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTCTCcca                                                                      >  1:337134/1‑100 (MQ=255)
                          cATTGTGGCCGCCCCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                    <  1:3615135/101‑1 (MQ=255)
                          cATTGTGCCGCCCGCGCCCAAGAACGCGCGCACCGCCCCCTCAAGGATGGCGGGGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                    <  1:5157694/101‑1 (MQ=255)
                            ttGTGGCCGCCGCGCCCCAGCACGCGCCCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                                  <  1:4417229/101‑1 (MQ=255)
                             tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                 <  2:443031/101‑1 (MQ=255)
                              gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                                <  1:5314087/101‑1 (MQ=255)
                              gtgCCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                                <  2:3992957/101‑1 (MQ=255)
                                  ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                            <  1:2534568/101‑1 (MQ=255)
                                  ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                            <  2:4310560/101‑1 (MQ=255)
                                   cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                           <  1:2396328/101‑1 (MQ=255)
                                    gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                          >  1:336171/1‑101 (MQ=255)
                                      cgccCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGACGCCTGTC.TCCGACCTTTTCCCAGCg                                                        <  1:5375069/101‑1 (MQ=255)
                                        cgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                      <  1:1530436/101‑1 (MQ=255)
                                         gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                     <  1:5307808/101‑1 (MQ=255)
                                         cccccGGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                     <  2:4554107/100‑1 (MQ=255)
                                          ccccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                                    <  2:3707105/101‑1 (MQ=255)
                                            ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                  <  1:1161004/101‑1 (MQ=255)
                                             cAGACCCCGCGCCCCGGCCCGTCAAGGATGGCGGCGTCCC.GGGAATGCGGGCG.GACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                 <  2:3311291/101‑1 (MQ=255)
                                             cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcccc                                                 >  1:1891351/1‑100 (MQ=255)
                                             cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                 <  2:4993204/101‑1 (MQ=255)
                                             cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                 <  1:3200230/101‑1 (MQ=255)
                                              aGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                <  1:1594625/101‑1 (MQ=255)
                                              aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                <  1:2211518/101‑1 (MQ=255)
                                              aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                <  1:4190888/101‑1 (MQ=255)
                                               gAACGCGCGCCCCGGCCCCTAAAGGATGGCGGC.GCCCGGGAAAGGCGTGC.AGTCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                               <  2:1345177/101‑1 (MQ=255)
                                                  cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                            >  1:763764/1‑101 (MQ=255)
                                                  cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                            <  1:5024757/101‑1 (MQ=255)
                                                    cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCGC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACggg                                          <  1:1881856/101‑1 (MQ=255)
                                                    cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACggg                                          <  1:5658891/101‑1 (MQ=255)
                                                      cgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGGCggggg                                        >  1:3473449/1‑100 (MQ=255)
                                                      ccccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGt                                        <  1:2801830/99‑1 (MQ=255)
                                                       gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                       <  2:5466480/101‑1 (MQ=255)
                                                       cccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                       <  1:1114082/100‑1 (MQ=255)
                                                        ccgcGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                      <  1:2505919/98‑1 (MQ=255)
                                                        ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                      <  1:2328484/101‑1 (MQ=255)
                                                          ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                                    >  1:4696945/1‑101 (MQ=255)
                                                          ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGa                                    >  1:5074847/1‑101 (MQ=255)
                                                           cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                   <  2:4154972/101‑1 (MQ=255)
                                                           cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                   >  2:2046264/1‑101 (MQ=255)
                                                            ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                  >  2:4447731/1‑101 (MQ=255)
                                                            ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCCACCTTTTCCCCGCGCAACCCCCGCCtgggggtcccg                                  >  1:464294/1‑94 (MQ=255)
                                                              ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGGCCTTTTCCCCGCGGATCCCGCGACGGGGGGCGCCGcc                                >  2:3556393/1‑101 (MQ=255)
                                                              ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                                <  2:5307257/101‑1 (MQ=255)
                                                                ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                              <  1:2078597/101‑2 (MQ=255)
                                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTG.CCTCCGGCCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                            <  2:942211/101‑4 (MQ=255)
                                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGCGGATCCCGCGACGGGGTGCGACGCCtgcg                            >  1:3895935/1‑98 (MQ=255)
                                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                            <  1:5335445/101‑4 (MQ=255)
                                                                   cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTTCGACGCCtgcgg                           <  1:3121994/101‑5 (MQ=255)
                                                                   cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcgg                           >  1:1007920/1‑97 (MQ=255)
                                                                   cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCCtgcgg                           >  1:1230892/1‑97 (MQ=255)
                                                                    aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgaggc                          >  2:606965/1‑96 (MQ=255)
                                                                    aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                         <  2:5122248/101‑1 (MQ=255)
                                                                    aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGAAGCCtcgggc                          >  2:1103977/1‑96 (MQ=255)
                                                                    aaGGAGGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                         <  2:1988621/101‑1 (MQ=255)
                                                                       gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                      >  2:2590661/1‑101 (MQ=255)
                                                                       gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAGCCCGCGCCGGGGGGCGCCGCC.TGGGGcgcc                      >  1:2291187/1‑101 (MQ=255)
                                                                        aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccc                     >  2:1677447/1‑100 (MQ=255)
                                                                         tGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGCC.TGAGGcgccgc                    >  1:793200/1‑101 (MQ=255)
                                                                          ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCgccgcc                   >  1:4051030/1‑101 (MQ=255)
                                                                           gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                  <  1:404067/101‑1 (MQ=255)
                                                                             ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCgc                >  2:4641724/1‑100 (MQ=255)
                                                                                  gcccgggAATGCGGGC.ATACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgg            <  2:3068151/97‑1 (MQ=255)
                                                                                   cccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGCCGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggg           >  1:1490507/4‑101 (MQ=255)
                                                                                   cccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggg           >  2:3187858/4‑101 (MQ=255)
                                                                                    ccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgggg          <  2:3076225/99‑1 (MQ=255)
                                                                                     cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAcgggg         >  2:1694313/2‑101 (MQ=255)
                                                                                       ggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc       <  2:3353610/101‑1 (MQ=255)
                                                                                        gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGGGACGCC.TGCGGCGCCGCCCGaacagaggagg      >  2:4411563/1‑92 (MQ=255)
                                                                                        gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt      <  2:4975088/101‑1 (MQ=255)
                                                                                        gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt      <  1:3411221/101‑1 (MQ=255)
                                                                                        gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt      <  1:1722106/101‑1 (MQ=255)
                                                                                          aTGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc    <  1:5534670/101‑1 (MQ=255)
                                                                                            gCGGGC.AGCCCGGGCC.GCCCGCCGCCTGTC.TCCATCCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcgc  <  1:4969167/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
GATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGC  >  NC_014034/1607818‑1608014

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 39 ≤  ATCG