BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,206	Δ1 bp	100%	intergenic (‑134/+91)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,206	0	G→.	100.0%	704.1	203	intergenic (‑134/+91)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (88/113): ref base (2/0): total (90/113)

Only 100 of 209 total aligned reads displayed.


GTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696106‑1696300


gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAAGgg                                                                                               >  1:1727236/1‑101 (MQ=255)


 tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGCGG..TGCCCCCCCCGGCCCTTCG.GCCCATTCACCCCCGGGTGTTTGGGCCAAGggg                                                                                              >  2:2063718/1‑101 (MQ=255)


     aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                        <  1:5656327/101‑1 (MQ=16)


      tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       >  1:5313002/1‑100 (MQ=255)


      tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       >  2:1849712/1‑100 (MQ=255)


       ttCGAATCCGTGCGCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                      <  1:1574542/101‑3 (MQ=255)


        tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     >  2:5760377/1‑98 (MQ=255)


        tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     <  1:974318/101‑4 (MQ=12)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                   <  2:725613/101‑6 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                   >  2:2250233/1‑96 (MQ=12)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                   <  2:2394705/101‑6 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGCCGC.TGCCCCTCCTCGCCTGCCG.GCCAACTCACCCCCGGATGTTTGGGGCAA..GGTGAAacacctt                                                                                   >  1:470609/1‑96 (MQ=255)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGCC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  2:382282/1‑95 (MQ=255)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  2:711231/1‑95 (MQ=255)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  <  2:425801/101‑7 (MQ=12)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  2:2554176/1‑95 (MQ=255)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTCGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacagcttg                                                                                  >  2:792852/1‑95 (MQ=255)


            aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                 <  2:545941/101‑8 (MQ=255)


             tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                >  2:3157585/1‑93 (MQ=12)


             tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                <  2:4404361/101‑9 (MQ=255)


               cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                              >  1:5080158/1‑91 (MQ=255)


                  gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                         <  2:3555451/101‑1 (MQ=255)


                  gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                         <  2:1598833/101‑1 (MQ=255)


                   cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                        <  1:289385/101‑1 (MQ=255)


                   cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                        <  1:2209872/101‑1 (MQ=255)


                    cgatcttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       <  1:1522735/96‑1 (MQ=2)


                    aCGAGTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       <  2:2641926/101‑1 (MQ=2)


                    aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       >  2:3291777/1‑101 (MQ=255)


                     cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                      <  1:5175985/101‑1 (MQ=255)


                     cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                      >  1:101160/1‑101 (MQ=255)


                           ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                >  2:3608866/1‑101 (MQ=255)


                            tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                               <  1:4084682/101‑1 (MQ=255)


                            tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                               <  2:448122/101‑1 (MQ=255)


                            tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                               <  1:3894203/101‑1 (MQ=255)


                             gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              >  1:376425/1‑101 (MQ=255)


                              cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  2:5337219/101‑1 (MQ=255)


                              cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  2:347716/101‑1 (MQ=255)


                              cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCCTTCG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             >  1:4191496/1‑101 (MQ=255)


                                aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                           <  1:4121887/101‑1 (MQ=9)


                                 ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                          >  2:1906649/1‑101 (MQ=255)


                                 ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                          >  1:2305146/1‑101 (MQ=255)


                                  gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                         >  1:3505289/1‑101 (MQ=9)


                                   gggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                        <  2:4710891/100‑1 (MQ=9)


                                   aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCCCCCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCTCct                                                        >  1:792507/1‑101 (MQ=255)


                                    ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                       <  2:4588254/101‑1 (MQ=255)


                                    ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                       <  2:484049/101‑1 (MQ=255)


                                     gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                      <  2:1376288/101‑1 (MQ=255)


                                          agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                                   <  1:3006840/91‑1 (MQ=255)


                                          agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                                   <  2:286209/91‑1 (MQ=255)


                                           gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  <  2:1624173/92‑1 (MQ=9)


                                           gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  >  1:1590564/10‑101 (MQ=16)


                                           gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  >  1:815329/10‑101 (MQ=16)


                                           gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGATTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  >  2:5422282/10‑101 (MQ=255)


                                            aggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                 >  1:5508406/9‑101 (MQ=16)


                                             ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  1:5708334/94‑1 (MQ=255)


                                             ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  1:4797495/94‑1 (MQ=255)


                                               ggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                              <  1:2459546/96‑1 (MQ=255)


                                                 cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            <  2:4064968/98‑1 (MQ=16)


                                                 cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            <  2:1586700/98‑1 (MQ=16)


                                                 cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            >  2:2899745/4‑101 (MQ=16)


                                                 cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            <  2:2452049/98‑1 (MQ=16)


                                                  gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                           >  2:433399/3‑101 (MQ=16)


                                                    tGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                         <  1:5072981/101‑1 (MQ=16)


                                                     gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                        <  1:5786815/101‑1 (MQ=255)


                                                     gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                        <  1:2856284/101‑1 (MQ=255)


                                                      ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       <  1:1485546/101‑1 (MQ=255)


                                                      ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaac                                       >  1:2721148/1‑100 (MQ=16)


                                                      ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       <  1:5344937/101‑1 (MQ=255)


                                                       cccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                      <  1:869452/101‑1 (MQ=16)


                                                       cccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAACGGCAAGc                                      <  2:1178357/101‑1 (MQ=16)


                                                         cTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                                    >  1:2585213/1‑101 (MQ=16)


                                                             tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                >  1:2523580/1‑101 (MQ=255)


                                                             tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                <  2:3108288/101‑1 (MQ=19)


                                                              ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               <  1:4809667/101‑1 (MQ=19)


                                                              ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               >  1:1394413/1‑101 (MQ=255)


                                                              ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               >  2:5158674/1‑101 (MQ=255)


                                                              ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               <  2:4529224/101‑1 (MQ=19)


                                                                 cTTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            <  1:1336457/101‑1 (MQ=25)


                                                                 cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            >  1:2404714/1‑101 (MQ=19)


                                                                 cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            >  2:272152/1‑101 (MQ=255)


                                                                 cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTCTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            <  2:5010192/101‑1 (MQ=255)


                                                                  tttCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           <  1:314366/101‑1 (MQ=25)


                                                                  tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           >  1:1449903/1‑101 (MQ=255)


                                                                  tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           >  1:3979311/1‑101 (MQ=255)


                                                                  tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           <  1:5422572/101‑1 (MQ=19)


                                                                           aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGacc                   >  2:2506966/1‑101 (MQ=255)


                                                                            attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                  >  1:4486051/7‑101 (MQ=255)


                                                                            aTTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                  <  1:3609094/101‑1 (MQ=25)


                                                                             ttCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                 <  1:1045556/101‑1 (MQ=25)


                                                                              tCCCCCGGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                <  2:4577258/101‑1 (MQ=255)


                                                                              tCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGTTTTTTCTGGGCGCAAATCCCCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                <  1:3998092/101‑1 (MQ=255)


                                                                               caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt               >  2:456789/4‑101 (MQ=25)


                                                                                accccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGAAAATACGCCTCTGGCCGGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg              <  1:3291977/99‑1 (MQ=255)


                                                                                 ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa             >  1:3571735/2‑101 (MQ=255)


                                                                                  cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt            >  1:1948850/1‑101 (MQ=255)


                                                                                     gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt         <  2:1116139/101‑1 (MQ=255)


                                                                                       gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCACGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc       >  1:4968728/6‑101 (MQ=255)


                                                                                       gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc       >  1:4744639/6‑101 (MQ=255)


                                                                                        atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGGGcc      >  2:5706908/5‑101 (MQ=255)


                                                                                          gtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    <  2:1724262/99‑1 (MQ=37)


GTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696106‑1696300

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG