Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,214 Δ1 bp 100% intergenic (‑142/+83) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,2140C→.100.0% 20.2 178intergenic (‑142/+83)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (77/92):  ref base (0/9):  total (77/101)
Fisher's exact test strand distribution p-value = 5.59e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.19e-06

Only 100 of 213 total aligned reads displayed.

ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.C.AATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCC  >  NC_014034/1696111‑1696309
                                                                                                         |                                                                                               
 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                                <  1:3820604/101‑2 (MQ=255)
 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                                >  1:5313002/1‑100 (MQ=255)
  ttCGAATCCGTGCGCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                               <  1:1574542/101‑3 (MQ=255)
   tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                              <  1:974318/101‑4 (MQ=12)
   tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                              >  2:5760377/1‑98 (MQ=255)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                            >  2:2250233/1‑96 (MQ=12)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                            <  2:2394705/101‑6 (MQ=255)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                            <  2:725613/101‑6 (MQ=255)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                           >  2:711231/1‑95 (MQ=255)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTCGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacagcttg                                                                                           >  2:792852/1‑95 (MQ=255)
       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCGAA..GGTGAAacaccttgc                                                                                          >  1:4711172/1‑94 (MQ=255)
       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                          <  2:545941/101‑8 (MQ=255)
        tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                         <  1:277363/101‑9 (MQ=255)
         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                        >  2:2540259/1‑92 (MQ=255)
         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                        <  2:4833660/101‑10 (MQ=9)
          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                                       <  1:1663861/101‑11 (MQ=255)
          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                                       >  1:5080158/1‑91 (MQ=255)
             gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                  <  1:4745554/101‑1 (MQ=255)
              cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                                 <  1:2889633/101‑1 (MQ=255)
               tctaaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                                <  1:4534363/98‑1 (MQ=2)
               cgatcttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                                <  1:1522735/96‑1 (MQ=2)
                cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                               <  2:2720343/101‑1 (MQ=255)
                cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                               <  1:5175985/101‑1 (MQ=255)
                cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                               >  1:101160/1‑101 (MQ=255)
                   aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                                            <  2:1421258/101‑1 (MQ=255)
                      ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                         >  2:3608866/1‑101 (MQ=255)
                      ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                         >  1:4806610/1‑101 (MQ=255)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                        <  1:3894203/101‑1 (MQ=255)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                        <  1:4084682/101‑1 (MQ=255)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                        >  1:4960620/1‑101 (MQ=9)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                        <  1:2915783/101‑1 (MQ=255)
                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                       <  2:3500168/101‑1 (MQ=9)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                      <  1:2215698/101‑1 (MQ=255)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                      >  1:447079/1‑101 (MQ=255)
                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                                     >  2:4138663/1‑101 (MQ=9)
                            ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGc                                                                   <  2:1359197/101‑1 (MQ=255)
                            ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                                   >  1:2305146/1‑101 (MQ=255)
                             gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                  >  1:3505289/1‑101 (MQ=9)
                              gggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                 <  2:4710891/100‑1 (MQ=9)
                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCCCCCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCTCct                                                                 >  1:792507/1‑101 (MQ=255)
                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                 <  1:3157774/101‑1 (MQ=9)
                               ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                                <  2:4588254/101‑1 (MQ=255)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                               >  2:314333/1‑101 (MQ=9)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                               <  1:3586142/101‑1 (MQ=255)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                               <  2:4290626/101‑1 (MQ=255)
                                     agaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                                            <  2:286209/91‑1 (MQ=255)
                                     agaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                                            >  2:389819/11‑101 (MQ=9)
                                      gaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                           >  1:815329/10‑101 (MQ=16)
                                      gaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGATTTTTCTTGGCGCAAATACGCCTCTGGcc                                                           >  2:5422282/10‑101 (MQ=255)
                                       aggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                          >  2:4356471/9‑101 (MQ=16)
                                        ggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                         <  1:5708334/94‑1 (MQ=255)
                                          ggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                                       <  1:2459546/96‑1 (MQ=255)
                                            cgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                     >  2:2899745/4‑101 (MQ=16)
                                            cgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                     <  2:2452049/98‑1 (MQ=16)
                                             gctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                                    <  2:1773231/99‑1 (MQ=255)
                                              ctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                                   <  1:1727542/100‑1 (MQ=255)
                                               tGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                  <  1:5072981/101‑1 (MQ=16)
                                                gCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCGTCTGGCCCGAAGGGCaa                                                 >  2:5195249/1‑101 (MQ=255)
                                                gCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                                 <  1:5786815/101‑1 (MQ=255)
                                                 ccccTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                <  2:332332/101‑1 (MQ=255)
                                                 ccccTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                <  1:1485546/101‑1 (MQ=255)
                                                 ccccTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                <  1:5344937/101‑1 (MQ=255)
                                                  cccTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                               <  1:869452/101‑1 (MQ=16)
                                                      cctGGCCTTTCGGCC.AATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                                           <  2:4897380/99‑1 (MQ=19)
                                                       ctGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc                                          >  2:161873/2‑101 (MQ=255)
                                                        tGGCCTTTCGGCC.AATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                         <  2:5060198/101‑1 (MQ=25)
                                                        tGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                         >  1:2523580/1‑101 (MQ=255)
                                                         ggCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                                        >  1:1394413/1‑101 (MQ=255)
                                                          gCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                                       <  2:4489525/101‑1 (MQ=19)
                                                          gCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                                       <  2:380442/101‑1 (MQ=19)
                                                           ccTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                                      <  1:2596340/101‑1 (MQ=19)
                                                            cTTTCGGCC.AATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                                     <  1:1336457/101‑1 (MQ=25)
                                                            cTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                                     >  1:2404714/1‑101 (MQ=19)
                                                            cTTTCGG.CAAATTCGCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                                     <  2:1668573/101‑1 (MQ=19)
                                                             tttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                    >  1:1449903/1‑101 (MQ=255)
                                                             tttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                    >  1:312558/1‑101 (MQ=255)
                                                             tttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                    <  1:5422572/101‑1 (MQ=19)
                                                             tttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                    >  1:3979311/1‑101 (MQ=255)
                                                             tttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                    <  1:213777/101‑1 (MQ=19)
                                                                cggcc.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                                 <  2:3282864/97‑1 (MQ=21)
                                                                 ggcc.AATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGAAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                                <  2:2555716/98‑1 (MQ=255)
                                                                    c.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                             <  2:1590470/101‑1 (MQ=25)
                                                                      aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGacc                            >  2:2506966/1‑101 (MQ=255)
                                                                       attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                           >  2:1892533/7‑101 (MQ=255)
                                                                        ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                          >  2:441273/6‑101 (MQ=255)
                                                                        ttCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                          <  1:1045556/101‑1 (MQ=25)
                                                                         tCCCCCGGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                         <  2:4577258/101‑1 (MQ=255)
                                                                         tCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGTTTTTTCTGGGCGCAAATCCCCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                         <  1:3998092/101‑1 (MQ=255)
                                                                          caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                        >  2:456789/4‑101 (MQ=25)
                                                                             cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt                     <  1:5654469/101‑1 (MQ=37)
                                                                              ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc                    >  1:4138820/1‑101 (MQ=255)
                                                                                gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt                  <  2:1116139/101‑1 (MQ=255)
                                                                                   atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGGGcc               >  2:5706908/5‑101 (MQ=255)
                                                                                   atgtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc               >  1:3636559/5‑101 (MQ=255)
                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc             >  1:5486443/3‑101 (MQ=255)
                                                                                       ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg           >  1:3334677/1‑101 (MQ=255)
                                                                                             gccaaggTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGa       <  2:3062305/96‑1 (MQ=38)
                                                                                                 aggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgcgc   >  1:4598398/2‑101 (MQ=255)
                                                                                                 aggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgccc   >  2:1499860/2‑99 (MQ=255)
                                                                                                  ggTGAAAC..CCCTTGCTTTTTTTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGcc  <  2:2125981/101‑1 (MQ=255)
                                                                                                         |                                                                                               
ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.C.AATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCC  >  NC_014034/1696111‑1696309

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG