Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,849	Δ1 bp	100%	intergenic (+122/‑383)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,849	0	C→.	100.0%	284.8	71	intergenic (+122/‑383)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (13/56):  ref base (2/0):  total (15/56)

ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCG.CCCCGCAC.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGG  >  NC_014034/2185751‑2185945                                                                                                      |                                                                                                    aCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.c                                                                                                   <  1:3980354/101‑1 (MQ=255) aCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.c                                                                                                   <  2:3082272/101‑1 (MQ=255) aCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.c                                                                                                   <  1:2351044/101‑1 (MQ=255)  cAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.cc                                                                                                  <  1:5409499/101‑1 (MQ=255)  cAGGCGGCCCCGCGCCCCCCGGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.cc                                                                                                  <  1:682783/101‑1 (MQ=37)   aGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGCCGGGGGCG.CGC.CCCCGCAc.ccc                                                                                                 >  2:3209135/1‑101 (MQ=255)   aGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.ccc                                                                                                 <  1:725627/101‑1 (MQ=255)    ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.cccc                                                                                                <  2:4234162/101‑1 (MQ=255)     gcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.ccccg                                                                                               <  2:669640/101‑2 (MQ=255)      cggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.ccccgg                                                                                              <  1:2568240/101‑3 (MQ=255)      cggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.ccccgg                                                                                              <  2:4473393/101‑3 (MQ=255)      cggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAC.CCCCCg                                                                                              >  1:1430521/1‑101 (MQ=255)       ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCAc.ccccggc                                                                                             <  1:4476191/101‑4 (MQ=255)        gcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGACGCCCCTG.CGGGGGCT.CCGCCCCCGCAc.ccccggcg                                                                                            <  2:351990/101‑5 (MQ=37)         ccccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGt                                                                                          <  1:3308361/101‑1 (MQ=255)           ccGCCCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTAt                                                                                        <  2:1771500/101‑1 (MQ=37)            cGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTAtt                                                                                       <  2:4060896/101‑1 (MQ=255)            cGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTAtt                                                                                       <  1:1785390/101‑1 (MQ=255)             gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTAttt                                                                                      <  1:3770791/101‑1 (MQ=255)                ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATc                                                                                   <  2:5676942/101‑1 (MQ=255)                 cctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATcc                                                                                  >  1:2492362/1‑101 (MQ=255)                  ctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATccc                                                                                 <  2:3379413/101‑1 (MQ=255)                   tcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCG.CCCCCGCC.CCCCCCGCGTATTTATCCCa                                                                                >  2:4012679/1‑101 (MQ=38)                   tcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGCCTCCCC.CCCCGCAC.CCCCC.GCGGATTTATcccc                                                                                >  1:5025860/1‑100 (MQ=37)                   ccccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCa                                                                                <  2:5721695/100‑1 (MQ=37)                    cccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGTCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCaa                                                                               <  2:5740653/101‑1 (MQ=37)                      cgggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGa                                                                             <  1:1569809/101‑1 (MQ=255)                        ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGaaa                                                                           <  2:2803171/101‑1 (MQ=255)                         gcgggGCCGCCTGCTGTGCAAAGCGGTTCCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGaaaa                                                                          <  1:3027540/101‑1 (MQ=25)                           ggggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAag                                                                        <  1:3828696/101‑1 (MQ=255)                           ggggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAag                                                                        >  2:4049124/1‑101 (MQ=255)                           ggggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAag                                                                        <  1:5004906/101‑1 (MQ=255)                            gggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAagc                                                                       <  2:1561103/101‑1 (MQ=255)                             ggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAagca                                                                      <  2:3321431/101‑1 (MQ=255)                                cgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCg                                                                   <  1:1288714/101‑1 (MQ=255)                                cgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCg                                                                   <  1:1054916/101‑1 (MQ=255)                                    tGATGTGCAAAGCGGTTTCGCCTCGGCGGGGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCtt                                                               <  1:544948/101‑1 (MQ=37)                                     gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTg                                                              <  1:3210956/101‑1 (MQ=255)                                     gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTg                                                              <  1:3186460/101‑1 (MQ=255)                                         tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcccc                                                          <  1:4098633/101‑1 (MQ=255)                                            aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                                       <  2:4048711/101‑1 (MQ=255)                                            aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                                       <  2:5358368/101‑1 (MQ=255)                                             aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAg                                                      <  1:4578661/101‑1 (MQ=255)                                             aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAg                                                      <  1:4576875/101‑1 (MQ=255)                                              aGCGGTTTCGCCCCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAgg                                                     <  2:868332/101‑1 (MQ=37)                                               gCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.c                                                   <  1:124589/101‑1 (MQ=255)                                               gCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.c                                                   <  1:3432072/101‑1 (MQ=255)                                                 tgTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCt                                                 <  2:2361161/100‑1 (MQ=37)                                                 ggTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGG.CCt                                                 >  1:5287716/1‑101 (MQ=37)                                                  gTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCtt                                                <  1:1388983/101‑1 (MQ=255)                                                  gTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCtt                                                <  1:4035730/101‑1 (MQ=255)                                                  gTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCtt                                                <  2:3158185/101‑1 (MQ=255)                                                  gTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCtt                                                <  2:3130789/101‑1 (MQ=255)                                                  gTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCtt                                                <  1:5105344/101‑1 (MQ=255)                                                     tCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGtt                                             <  1:415262/101‑1 (MQ=255)                                                      cGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttt                                            <  1:3209324/101‑1 (MQ=255)                                                         cTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttttga                                         <  1:761523/101‑3 (MQ=37)                                                           cggcggTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttttgatg                                       <  1:3519361/101‑5 (MQ=37)                                                              cggTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAAAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGca                                   <  2:1880420/101‑1 (MQ=37)                                                               ggTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGcgc                                  >  2:3896873/1‑99 (MQ=25)                                                               ggTGCCGAGGCGACCCTG.CGGGGGCT.CCG.CCCC.CACACCCCCGGCGTATTTATCCCAACAAAAAGCAGCGGCTTGCCCCGCAGGCCCTTGttttctgggac                                    >  2:1348109/1‑94 (MQ=25)                                                                gTGCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGcaca                                 <  1:633599/101‑1 (MQ=37)                                                                 ggCCGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACaa                                <  1:4910950/100‑1 (MQ=37)                                                                    cGAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaa                             <  1:296721/101‑1 (MQ=37)                                                                     gggtcGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaaa                            <  1:5030694/97‑1 (MQ=25)                                                                     gAGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaaa                            <  1:1784450/101‑1 (MQ=37)                                                                      aGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAc                           <  2:2188675/101‑1 (MQ=37)                                                                      aGGCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGAGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAc                           >  2:1668104/1‑101 (MQ=25)                                                                       ggCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACg                          <  2:1887922/101‑1 (MQ=37)                                                                        gCGGCCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGc                         <  1:1902290/101‑1 (MQ=25)                                                                        gCGACCCTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGc                         <  2:2353874/101‑1 (MQ=37)                                                                            cccTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGaa                     <  2:484047/101‑1 (MQ=37)                                                                              cTG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGGCCCGCAGG.CCCTG.TTTTGATGGCACAAGAAACACCGAAcc                   >  1:696505/1‑101 (MQ=21)                                                                               tG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCTGCTTGCCCCGAAGG.CCTTG.GTTTGATGGCACAAGAAACGCCGAACCg                  <  2:2552738/101‑1 (MQ=25)                                                                               tG.CGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                  <  1:1650831/101‑1 (MQ=37)                                                                                  cGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc               <  2:4176632/101‑1 (MQ=37)                                                                                  cGGGGGCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc               >  2:2101707/1‑101 (MQ=37)                                                                                     gggCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccc            <  1:3041073/101‑2 (MQ=37)                                                                                      ggCT.CCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccct           <  1:5353620/101‑3 (MQ=37)                                                                                         cgatctccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCcttt         <  1:5273938/95‑2 (MQ=38)                                                                                          tccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc        >  1:3924062/6‑99 (MQ=37)                                                                                          tccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCCTGTTTTTG.TGGCAAAAGAAACGCCGAACCG.CCCCctttc        >  1:4397715/6‑99 (MQ=25)                                                                                           ccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa        >  2:3966850/5‑101 (MQ=38)                                                                                               ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCtt    <  1:5422582/101‑1 (MQ=38)                                                                                               ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttagatcg   >  2:5273648/1‑94 (MQ=255)                                                                                               ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAAGCCGAACCGCCCCCTTTCACCtt    >  1:3989617/1‑101 (MQ=38)                                                                                                 ccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCCAACCGCCCCCTTTCACCATgg  >  2:5709316/1‑101 (MQ=37)                                                                                                      |                                                                                                    ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGGGGGCT.CCG.CCCCGCAC.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGG  >  NC_014034/2185751‑2185945 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG