Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,902	Δ1 bp	100%	intergenic (+175/‑330)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,902	0	T→.	100.0%	260.6	62	intergenic (+175/‑330)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (24/38):  ref base (0/0):  total (24/38)

TCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCAC.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185803‑2185988                                                                                                       |                                                                                        tCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGtt                                                                                        <  1:415262/101‑1 (MQ=255)  cGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttt                                                                                       <  1:3209324/101‑1 (MQ=255)     cTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttttga                                                                                    <  1:761523/101‑3 (MQ=37)       cggcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGttttgatg                                                                                  <  1:3519361/101‑5 (MQ=37)          cggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAAAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGca                                                                              <  2:1880420/101‑1 (MQ=37)           ggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGcgc                                                                             >  2:3896873/1‑99 (MQ=25)           ggTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCC.CACACCCCCGGCGTATTTATCCCAACAAAAAGCAGCGGCTTGCCCCGCAGGCCCTTGttttctgggac                                                                               >  2:1348109/1‑94 (MQ=25)            gTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGcaca                                                                            <  1:633599/101‑1 (MQ=37)             ggCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACaa                                                                           <  1:4910950/100‑1 (MQ=37)                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaa                                                                        <  1:296721/101‑1 (MQ=37)                 gggtcGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaaa                                                                       <  1:5030694/97‑1 (MQ=25)                 gAGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGaaa                                                                       <  1:1784450/101‑1 (MQ=37)                  aGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAc                                                                      <  2:2188675/101‑1 (MQ=37)                  aGGCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGAGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAc                                                                      >  2:1668104/1‑101 (MQ=25)                   ggCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACg                                                                     <  2:1887922/101‑1 (MQ=37)                    gCGGCCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGc                                                                    <  1:1902290/101‑1 (MQ=25)                    gCGACCCTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGc                                                                    <  2:2353874/101‑1 (MQ=37)                        cccTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGaa                                                                <  2:484047/101‑1 (MQ=37)                          cTGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGGCCCGCAGG.CCCTG.TTTTGATGGCACAAGAAACACCGAAcc                                                              >  1:696505/1‑101 (MQ=21)                           tGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCTGCTTGCCCCGAAGG.CCTTG.GTTTGATGGCACAAGAAACGCCGAACCg                                                             <  2:2552738/101‑1 (MQ=25)                           tGCGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                                                             <  1:1650831/101‑1 (MQ=37)                             cGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc                                                          >  2:2101707/1‑101 (MQ=37)                             cGGGGGCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc                                                          <  2:4176632/101‑1 (MQ=37)                                gggCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccc                                                       <  1:3041073/101‑2 (MQ=37)                                 ggCTCCGCCCCCGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccct                                                      <  1:5353620/101‑3 (MQ=37)                                   cgatctccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCcttt                                                    <  1:5273938/95‑2 (MQ=38)                                    tccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc                                                   >  1:3924062/6‑99 (MQ=37)                                    tccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCCTGTTTTTG.TGGCAAAAGAAACGCCGAACCG.CCCCctttc                                                   >  1:4397715/6‑99 (MQ=25)                                     ccgcccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa                                                   >  2:3966850/5‑101 (MQ=38)                                         ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCtt                                               <  1:5422582/101‑1 (MQ=38)                                         ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttagatcg                                              >  2:5273648/1‑94 (MQ=255)                                         ccccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAAAGCCGAACCGCCCCCTTTCACCtt                                               >  1:3989617/1‑101 (MQ=38)                                           ccGCA..CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCCAACCGCCCCCTTTCACCATgg                                             >  2:5709316/1‑101 (MQ=37)                                              cgcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAAAAACGCCGAACCGCCCCCTTTCACCTTGGc                                            >  1:859876/5‑101 (MQ=255)                                                cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTc                                          >  2:2041202/3‑101 (MQ=255)                                                cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTc                                          <  1:962342/99‑1 (MQ=39)                                                cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTc                                          >  1:5270822/3‑101 (MQ=39)                                                 gcatccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCa                                         <  1:2258579/97‑1 (MQ=255)                                                 acccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.GCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCa                                         >  2:4268655/2‑101 (MQ=38)                                                   ccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCaaa                                       >  1:5002264/1‑101 (MQ=255)                                                     tctgCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAata                                     <  2:3572270/98‑1 (MQ=255)                                                        gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAataaga                                  >  1:3572487/1‑98 (MQ=255)                                                         cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 <  2:4397448/101‑1 (MQ=255)                                                         cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 >  2:5598355/1‑101 (MQ=255)                                                          gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                <  2:1573167/101‑1 (MQ=255)                                                           tagttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                               >  1:3297020/4‑101 (MQ=255)                                                           tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCCAATATCCCgg                               >  2:5488185/1‑101 (MQ=255)                                                            aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCggg                              <  2:4008703/101‑1 (MQ=255)                                                               tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCAAATATCCCgggggg                           >  2:4169457/1‑100 (MQ=255)                                                               tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGt                           >  2:324286/1‑101 (MQ=255)                                                                 tCCGAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:4268915/101‑1 (MQ=38)                                                                 tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:5488475/101‑1 (MQ=255)                                                                 tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  2:2336895/101‑1 (MQ=255)                                                                 tCCCAAGAAAAAGCAGCGGCTGGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:5415304/101‑1 (MQ=38)                                                                  cccAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                        >  1:5465545/1‑101 (MQ=255)                                                                   ccAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgg                       <  2:859807/101‑1 (MQ=255)                                                                   ccAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgg                       <  1:1318127/101‑1 (MQ=255)                                                                    cAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggg                      <  2:2412847/101‑1 (MQ=255)                                                                     aaGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.GTTTGATGGGACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCCGGGGTCCgggg                     >  2:4290930/1‑101 (MQ=255)                                                                      aGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggggg                    <  1:2041319/101‑1 (MQ=255)                                                                        aaaaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                  <  2:4387174/101‑1 (MQ=255)                                                                        aaaaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                  >  1:4561913/1‑101 (MQ=255)                                                                         aaaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgg                 >  2:1065633/1‑101 (MQ=255)                                                                         aaaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGGCCGGGGGCgg                 >  2:2196355/1‑101 (MQ=38)                                                                          aaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                <  1:1976129/101‑1 (MQ=38)                                                                          aaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                >  1:2400417/1‑101 (MQ=255)                                                                          aaaGCAGCGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                <  2:756933/101‑1 (MQ=255)                                                                             gcagcGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcc             <  1:4696570/101‑1 (MQ=255)                                                                                gcGGCTTGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          <  1:812045/101‑3 (MQ=37)                                                                                     ttGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcgg     <  1:2101824/101‑8 (MQ=25)                                                                                     ttGCCCCGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcgg     >  2:3585605/1‑94 (MQ=25)                                                                                        ccccGAAGG.CCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCAAATACCCCGGGGGTCGGGGGGCGGAGcccccggcgggtc  <  2:2361569/101‑11 (MQ=18)                                                                                                       |                                                                                        TCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCAC.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGG.CCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185803‑2185988 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG