Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,420,306 Δ1 bp 2.4% intergenic (‑100/+92) RCAP_rcc02245 ← / ← cysE2 major membrane protein I/serine O‑acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,420,3060G→. 2.4% 9.9 ‑2.4 ‑1.4252intergenic (‑100/+92)RCAP_rcc02245/cysE2major membrane protein I/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (103/143):  total (108/144)
Fisher's exact test strand distribution p-value = 8.69e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.61e-02

Only 100 of 254 total aligned reads displayed.

AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395
                                                                                                  |                                                                                         
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  <  1:2327604/101‑1 (MQ=255)
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  <  1:973828/101‑1 (MQ=255)
agccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.ACGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGt                                                                                  >  2:4138987/1‑101 (MQ=255)
 gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  2:5101777/101‑1 (MQ=255)
 gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  2:262687/101‑1 (MQ=255)
 accagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  2:1861390/100‑1 (MQ=255)
   cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               >  1:3131157/1‑101 (MQ=255)
   cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               >  2:330508/1‑101 (MQ=255)
     gcgcGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTc                                                                             <  2:3863621/101‑1 (MQ=255)
     gcgcGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTc                                                                             <  1:5173509/101‑1 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         <  1:254362/98‑1 (MQ=255)
           gcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGtt                                                                        <  2:2079987/99‑1 (MQ=255)
             gTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtct                                                                      >  1:2921666/1‑101 (MQ=255)
             gTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtct                                                                      <  2:3609627/101‑1 (MQ=255)
              tGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     <  1:146792/101‑1 (MQ=255)
              ctaTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     <  2:3651188/99‑1 (MQ=255)
               gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:1819779/1‑101 (MQ=255)
                aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCCG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct                                                                   >  1:238375/1‑101 (MQ=255)
                aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctcag                                                                   >  1:3651416/1‑99 (MQ=255)
                aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct                                                                   <  1:5660246/101‑1 (MQ=255)
                 tCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCctt                                                                  <  1:1850176/101‑1 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                <  1:3798971/101‑1 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                <  1:17419/101‑1 (MQ=255)
                      gggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTg                                                              <  2:4843253/98‑1 (MQ=255)
                         cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggt                                                           <  2:2696531/101‑2 (MQ=255)
                         cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggt                                                           <  1:4060733/101‑2 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          >  1:344380/6‑99 (MQ=255)
                            ggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgc                                                         <  1:5772006/97‑4 (MQ=255)
                            ggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtgc                                                         >  1:744271/5‑98 (MQ=255)
                              atcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAg                                                      <  2:1113647/99‑1 (MQ=255)
                              atcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAg                                                      <  1:1012218/99‑1 (MQ=255)
                              atcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAg                                                      >  2:3264152/3‑101 (MQ=255)
                              atcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCATTCTT.GGGTGCAg                                                      <  1:2090248/99‑1 (MQ=255)
                               tcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGa                                                     <  1:1946602/100‑1 (MQ=255)
                                  ggcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaa                                                   <  1:4553023/97‑1 (MQ=255)
                                   gcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaaa                                                  >  1:4282339/4‑101 (MQ=255)
                                    cgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAc                                                 >  1:5272689/3‑101 (MQ=255)
                                     gccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAcc                                                <  2:3148404/100‑1 (MQ=255)
                                      ccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCa                                               <  2:3088690/101‑1 (MQ=255)
                                       cGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCAt                                              <  2:2034178/101‑1 (MQ=255)
                                       cGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCAt                                              <  2:3925920/101‑1 (MQ=255)
                                       cGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCAt                                              >  1:5281108/1‑101 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  1:1119349/101‑1 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  1:4398928/101‑1 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  1:37104/101‑1 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           >  1:4246866/1‑101 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           <  2:4658784/101‑1 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           <  2:1212569/101‑1 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           >  1:974961/1‑101 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGCTCTCCTTCTT.GGATGCAGAAAACCATccctc                                         >  1:1358247/1‑99 (MQ=255)
                                             cGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCCGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgcg                                        >  1:570584/1‑101 (MQ=255)
                                               tGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGa                                      >  1:3082437/1‑101 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  2:4293564/97‑1 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                >  2:5361122/5‑101 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  1:891067/97‑1 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  1:606841/97‑1 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  1:3381873/97‑1 (MQ=255)
                                                         caaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCcgcg                             <  2:1784645/100‑1 (MQ=255)
                                                           aGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCggg                           <  2:776996/101‑1 (MQ=255)
                                                            gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGa                          <  2:4558555/101‑1 (MQ=255)
                                                             cTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAt                         >  2:1273774/1‑101 (MQ=255)
                                                             cTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAt                         <  2:472741/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:2921499/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        >  2:1539601/1‑101 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:5647076/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  1:384032/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        >  1:399262/1‑101 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:5070904/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtg                        >  2:2844243/1‑100 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGGCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        >  1:1642355/1‑101 (MQ=255)
                                                               ggcgggcgGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTg                       <  2:5684383/101‑1 (MQ=255)
                                                               ggcgggcgGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTg                       <  1:2108205/101‑1 (MQ=255)
                                                                gcgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTgg                      <  1:905233/101‑1 (MQ=255)
                                                                 cgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGc                     <  1:3204016/101‑1 (MQ=255)
                                                                 cgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGc                     <  2:1664858/101‑1 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  1:1402852/101‑1 (MQ=255)
                                                                   ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   >  1:1092081/1‑101 (MQ=255)
                                                                    gcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAgg                  <  1:5524420/101‑1 (MQ=255)
                                                                    gcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAgg                  <  1:330552/101‑1 (MQ=255)
                                                                     cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  1:4191115/101‑1 (MQ=255)
                                                                     cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  2:2528491/101‑1 (MQ=255)
                                                                     cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  2:687755/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                <  2:5546956/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                <  2:193700/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                <  2:3442054/101‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             <  2:5280818/101‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGaa             >  1:1042070/1‑101 (MQ=255)
                                                                          cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaa            >  2:918874/1‑101 (MQ=255)
                                                                          cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaa            >  2:5789356/1‑101 (MQ=255)
                                                                            gTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAt          >  2:2207943/1‑101 (MQ=255)
                                                                             tCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtg         <  2:3014861/101‑1 (MQ=255)
                                                                              cGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtgt        <  1:549319/101‑1 (MQ=255)
                                                                              cGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtgt        <  2:1580574/101‑1 (MQ=255)
                                                                                cccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATgtgt      <  1:4797295/101‑1 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCACGGGATTGGCAGGTTGAAAATGTGtt     >  1:3479244/1‑101 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTT.TTTGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     >  1:2536540/1‑101 (MQ=255)
                                                                                   aGCGCCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGAAAATGTGTTCt   >  2:1308744/1‑101 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGACAATGTGTTCtt  >  2:4351673/1‑100 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  <  1:1201341/101‑1 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  >  1:2696660/1‑101 (MQ=255)
                                                                                                  |                                                                                         
AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG